{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T23:28:09Z","timestamp":1780442889312,"version":"3.54.1"},"reference-count":66,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2025,8,7]],"date-time":"2025-08-07T00:00:00Z","timestamp":1754524800000},"content-version":"vor","delay-in-days":7,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"DFG","doi-asserted-by":"publisher","award":["BO 2512\/8-1"],"award-info":[{"award-number":["BO 2512\/8-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Doctoral Programmes in Germany","award":["57588370"],"award-info":[{"award-number":["57588370"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,7,31]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The primary mRNA sequence determines its secondary structure and the repertoire of interacting RNA-binding proteins (RBPs). The resulting mRNA ribonucleoprotein complex (mRNP) then influences all stages of the life of an mRNA. Here, we determined the mRNP composition of individual Kaposi sarcoma herpesviral (KSHV) mRNAs. Like all herpesviruses, KSHV switches between a latent and lytic stage of the viral life cycle. During reactivation from latency, the viral RNA regulator ORF57 ensures the translation of viral mRNAs by increasing their mRNA stability and nuclear export. We optimized an LNA\/DNA mixmer RNA capture protocol for both transfection and viral infection settings. In combination with eCLIP, we confirmed that ORF57 directly binds to an AU-rich RNA motif, which may enable ORF57 to discriminate viral from cellular RNAs based on the nucleotide bias of KSHV lytic RNAs. In addition, we captured the RBPome of two ORF57-dependent viral transcripts and identified the host RNA processing factor SRSF3 as a key regulator of viral replication.<\/jats:p>","DOI":"10.1093\/pnasnexus\/pgaf225","type":"journal-article","created":{"date-parts":[[2025,8,12]],"date-time":"2025-08-12T18:56:54Z","timestamp":1755025014000},"source":"Crossref","is-referenced-by-count":2,"title":["RNA-interactome capture identifies SRSF3 as a key protein for herpesviral gene expression"],"prefix":"10.1093","volume":"4","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-7005-0086","authenticated-orcid":false,"given":"Carolin","family":"Vogt","sequence":"first","affiliation":[{"name":"Institute for Virology, Hannover Medical School , Hannover 30625 ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6819-4284","authenticated-orcid":false,"given":"Marco","family":"van Ham","sequence":"additional","affiliation":[{"name":"Cellular Proteomics, Helmholtz Centre for Infection Research , Braunschweig 38124 ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-7126-2990","authenticated-orcid":false,"given":"Ruchira","family":"Bhowmik","sequence":"additional","affiliation":[{"name":"Institute for Virology, Hannover Medical School , Hannover 30625 ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0412-003X","authenticated-orcid":false,"given":"Amir","family":"Argoetti","sequence":"additional","affiliation":[{"name":"Faculty of Biology, Technion\u2014Israel Institute of Technology , Haifa 320003 ,","place":["Israel"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4292-0599","authenticated-orcid":false,"given":"Daniel P","family":"Depledge","sequence":"additional","affiliation":[{"name":"Institute for Virology, Hannover Medical School , Hannover 30625 ,","place":["Germany"]},{"name":"German Center for Infection Research (DZIF), partner Site Hannover-Braunschweig , Hannover 38124 ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9142-7146","authenticated-orcid":false,"given":"Lars","family":"Steinbr\u00fcck","sequence":"additional","affiliation":[{"name":"Institute for Virology, Hannover Medical School , Hannover 30625 ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3891-5746","authenticated-orcid":false,"given":"Jasper","family":"G\u00f6tting","sequence":"additional","affiliation":[{"name":"Institute for Virology, Hannover Medical School , Hannover 30625 ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina","family":"Henkel","sequence":"additional","affiliation":[{"name":"Institute for Virology, Hannover Medical School , Hannover 30625 ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Cuadra Granados","sequence":"additional","affiliation":[{"name":"Institute for Virology, Hannover Medical School , Hannover 30625 ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8946-8237","authenticated-orcid":false,"given":"Yael","family":"Mandel-Gutfreund","sequence":"additional","affiliation":[{"name":"Faculty of Biology, Technion\u2014Israel Institute of Technology , Haifa 320003 ,","place":["Israel"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5655-1181","authenticated-orcid":false,"given":"Lothar","family":"J\u00e4nsch","sequence":"additional","affiliation":[{"name":"Cellular Proteomics, Helmholtz Centre for Infection Research , Braunschweig 38124 ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2800-9934","authenticated-orcid":false,"given":"Jens","family":"Bohne","sequence":"additional","affiliation":[{"name":"Institute for Virology, Hannover Medical School , Hannover 30625 ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,8,7]]},"reference":[{"key":"2025081306020477400_pgaf225-B1","doi-asserted-by":"crossref","first-page":"3101","DOI":"10.2741\/2912","article-title":"Processing of adeno-associated virus 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junctions","volume":"19","author":"Le Hir","year":"2000","journal-title":"EMBO J."},{"key":"2025081306020477400_pgaf225-B5","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1002\/rmv.387","article-title":"Split genes and their expression in Kaposi's sarcoma-associated herpesvirus","volume":"13","author":"Zheng","year":"2003","journal-title":"Rev Med Virol."},{"key":"2025081306020477400_pgaf225-B6","doi-asserted-by":"crossref","first-page":"33001","DOI":"10.1074\/jbc.M313008200","article-title":"The evolutionarily conserved Kaposi's sarcoma-associated herpesvirus ORF57 protein interacts with REF protein and acts as an RNA export factor","volume":"279","author":"Malik","year":"2004","journal-title":"J Biol Chem"},{"key":"2025081306020477400_pgaf225-B7","doi-asserted-by":"crossref","first-page":"5241","DOI":"10.2741\/3078","article-title":"The many roles of the regulatory protein ICP27 during herpes simplex virus 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