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However, fetal circadian rhythms are initially shielded from maternal entrainment, in addition to delayed circadian clock emergence due to CLOCK suppression. Premature CLOCK\/BMAL1 activation disrupts Hes7-driven somite-like structure in gastruloids. Given the genomic proximity of Per1 to Hes7 and their transcriptional ripple effect, the physiological significance of delayed cell-autonomous circadian clock development and the temporal program of maternal\u2013fetal communication during the developmental process has remained unclear. Here, based on a marked decline in Hsd11b2, encoding a GC-inactivating 11\u03b2-HSD2 enzyme, during organogenesis, we performed split-litter embryo-transfer experiments in which Hsd11b2 knockout (KO) and wild-type embryos shared the same maternal environment. Amniotic fluid GCs remained low and arrhythmic under basal conditions. In contrast, maternal stress caused a pronounced GC surge and Per1 induction in KO, suggesting that 11\u03b2-HSD2 buffers acute maternal GC surges. Despite the genomic proximity of Per1 to Hes7 and their transcriptional ripple effect, stress-associated and pharmacological GC exposure recapitulated no overt segmentation defects in vivo. Embryonic stem cell\u2013derived gastruloid assays confirmed that neither GC exposure nor Per1 induction arrested Hes7 oscillations, whereas premature CLOCK\/BMAL1 activation impaired these processes even in Hes7 KO gastruloid with ectopic rescue, suggesting that interference with the segmentation clock is mediated by premature CLOCK\/BMAL1 activation, not by GC-induced Per1 expression. These findings clearly show that maternal GC signals are selectively buffered during early development. In addition, suppression of CLOCK\/BMAL1 activity preserves segmentation clock function, indicating delayed circadian clock emergence is actively regulated during embryogenesis.<\/jats:p>","DOI":"10.1093\/pnasnexus\/pgag286","type":"journal-article","created":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T11:06:11Z","timestamp":1787569571000},"source":"Crossref","is-referenced-by-count":0,"title":["11\u03b2-HSD2 buffers fetal glucocorticoid exposure inducing\n                    <i>Per1<\/i>\n                    expression under maternal stress"],"prefix":"10.1093","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6696-9429","authenticated-orcid":false,"given":"Kazuya","family":"Yabumoto","sequence":"first","affiliation":[{"name":"Department of Physiology and Systems Bioscience, Graduate School of Medical Science, Kyoto Prefectural University of Medicine , Kyoto 602-8566 ,","place":["Japan"]},{"name":"Department of Obstetrics and Gynecology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine , Kyoto 602-8566 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3202-1124","authenticated-orcid":false,"given":"Yasuhiro","family":"Umemura","sequence":"additional","affiliation":[{"name":"Department of Physiology and Systems Bioscience, Graduate School of Medical Science, Kyoto Prefectural University of Medicine , Kyoto 602-8566 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hitomi","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Laboratory of Integrative Biological Science, Institute for Life and Medical Sciences, Kyoto University , Kyoto 606-8507 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuhiro","family":"Endo","sequence":"additional","affiliation":[{"name":"Department of Physiology and Systems Bioscience, Graduate School of Medical Science, Kyoto Prefectural University of Medicine , Kyoto 602-8566 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobuya","family":"Koike","sequence":"additional","affiliation":[{"name":"Department of Physiology and Systems Bioscience, Graduate School of Medical Science, Kyoto Prefectural University of Medicine , Kyoto 602-8566 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akiyo","family":"Kakibuchi","sequence":"additional","affiliation":[{"name":"Department of Physiology and Systems Bioscience, Graduate School of Medical Science, Kyoto Prefectural University of Medicine , Kyoto 602-8566 ,","place":["Japan"]},{"name":"Department of Obstetrics and Gynecology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine , Kyoto 602-8566 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akira","family":"Sugimoto","sequence":"additional","affiliation":[{"name":"Department of Physiology and Systems Bioscience, Graduate School of Medical Science, Kyoto Prefectural University of Medicine , Kyoto 602-8566 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9792-4132","authenticated-orcid":false,"given":"Taisuke","family":"Mori","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine , Kyoto 602-8566 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2564-0149","authenticated-orcid":false,"given":"Gen","family":"Kondoh","sequence":"additional","affiliation":[{"name":"Laboratory of Integrative Biological Science, Institute for Life and Medical Sciences, Kyoto University , Kyoto 606-8507 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1889-7050","authenticated-orcid":false,"given":"Kazuhiro","family":"Yagita","sequence":"additional","affiliation":[{"name":"Department of Physiology and Systems Bioscience, Graduate School of Medical Science, Kyoto Prefectural University of Medicine , Kyoto 602-8566 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,8,24]]},"reference":[{"key":"2026090621070118700_pgag286-B1","doi-asserted-by":"crossref","DOI":"10.1242\/dev.201045","article-title":"On time: developmental timing within and across species","volume":"150","author":"Garcia-Ojalvo","year":"2023","journal-title":"Development"},{"key":"2026090621070118700_pgag286-B2","doi-asserted-by":"crossref","DOI":"10.1016\/j.gde.2024.102152","article-title":"Emergence of the circadian clock oscillation during the developmental process in 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