{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T16:17:59Z","timestamp":1790957879405,"version":"4.1.0"},"reference-count":41,"publisher":"Oxford University Press (OUP)","issue":"1199","license":[{"start":{"date-parts":[[2025,1,30]],"date-time":"2025-01-30T00:00:00Z","timestamp":1738195200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Ni\u011fde \u00d6mer Halisdemir University Scientific Research Projects Unit","award":["SAT 2022\/1 BAGEP"],"award-info":[{"award-number":["SAT 2022\/1 BAGEP"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,8,17]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Epoxyeicosatrienoic acids (EETs) are closely associated with lipoprotein metabolism, and changes in lipid profiles potentially affect their levels and functions. Given the alterations in lipid metabolism after cholecystectomy, this study aimed to investigate the levels of four EET regioisomers (free and esterified) and lipid profiles in patients with cholelithiasis after laparoscopic cholecystectomy (LC) and explore correlations between these parameters.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>This prospective study involved 40 patients with symptomatic cholelithiasis who underwent LC. Plasma EETs and serum total cholesterol, triglyceride, high-density lipoprotein (HDL), very low-density lipoprotein, low-density lipoprotein, and body mass index (BMI) values were determined preoperatively and after 6 months of LC.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>After LC, triglyceride and very low-density lipoprotein levels increased while TC decreased. BMI values increased significantly after the operation. Despite plasma EET levels decreasing remarkably after surgery, this change did not reach statistical significance. A significant correlation was observed between preoperative levels of 8,9- and 11,12-EET and pre-and post-operative HDL. There was a significant negative correlation between the EET levels measured before and after surgery and the change in BMI values.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>In this study, we observed significant changes in lipid profile 6 months after LC. While HDL, low-density lipoprotein, and EET levels showed a decreasing trend post-surgery, this change was not statistically significant. This trend and their significant correlations may indicate a complex relationship between HDL and EET metabolism. In addition, the negative correlation between EET levels and BMI changes highlights the need for further research to elucidate the metabolic impact and weight regulation of EETs after LC.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/postmj\/qgae175","type":"journal-article","created":{"date-parts":[[2025,1,29]],"date-time":"2025-01-29T07:25:19Z","timestamp":1738135519000},"page":"828-835","source":"Crossref","is-referenced-by-count":1,"title":["Effect of laparoscopic cholecystectomy on plasma levels of EETs, arachidonic acids derived lipid mediators"],"prefix":"10.1093","volume":"101","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3732-2102","authenticated-orcid":false,"given":"\u0130smail","family":"Sar\u0131","sequence":"first","affiliation":[{"name":"Department of Medical Biochemistry, Faculty of Medicine, K\u0131rklareli University , K\u0131rklareli, 39000 ,","place":["Turkey"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hac\u0131","family":"Bolat","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Private Ni\u011fde Hayat Hospital , Ni\u011fde, 51000 ,","place":["Turkey"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esma","family":"\u00d6zmen","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry, Faculty of Medicine, Ni\u011fde Omer Halisdemir University , Ni\u011fde, 51000 ,","place":["Turkey"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Burcu","family":"K\u00f6se","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Faculty of Arts and Sciences, Ni\u011fde \u00d6mer Halisdemir University , Ni\u011fde, 51000 ,","place":["Turkey"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Durmu\u015f","family":"Ayan","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry, Faculty of Medicine, Ni\u011fde Omer Halisdemir University , Ni\u011fde, 51000 ,","place":["Turkey"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,1,30]]},"reference":[{"key":"2025081806221593300_ref1","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1038\/labinvest.2017.95","article-title":"Cholecystectomy as a risk factor of metabolic syndrome: from epidemiologic clues to biochemical mechanisms","volume":"98","author":"Chen","year":"2018","journal-title":"Lab Investig"},{"key":"2025081806221593300_ref2","doi-asserted-by":"publisher","first-page":"e12983","DOI":"10.1111\/OBR.12983","article-title":"Pathophysiological connections between gallstone disease, insulin resistance, and obesity","volume":"21","author":"Cort\u00e9s","year":"2020","journal-title":"Obes Rev"},{"key":"2025081806221593300_ref3","doi-asserted-by":"publisher","first-page":"952","DOI":"10.1038\/AJG.2013.70","article-title":"Relationship of non-alcoholic fatty liver disease with cholecystectomy in the US population","volume":"108","author":"Ruhl","year":"2013","journal-title":"Am J Gastroenterol"},{"key":"2025081806221593300_ref4","doi-asserted-by":"publisher","first-page":"641","DOI":"10.1016\/j.ijsu.2011.08.003","article-title":"Association of dyslipidaemia with cholilithiasis and effect of cholecystectomy on the same","volume":"9","author":"Malik","year":"2011","journal-title":"Int J Surg"},{"key":"2025081806221593300_ref5","doi-asserted-by":"publisher","first-page":"97","DOI":"10.4103\/2278-9596.122926","article-title":"Effect of cholelithiasis and cholecystectomy on serum lipids and blood glucose parameters","volume":"3","author":"Jindal","year":"2013","journal-title":"Arch Int Surg"},{"key":"2025081806221593300_ref6","doi-asserted-by":"publisher","first-page":"1513","DOI":"10.1016\/J.HPB.2020.07.011","article-title":"Cholecystectomy as a risk factor for non-alcoholic fatty liver disease development","volume":"22","author":"Rodr\u00edguez-Antonio","year":"2020","journal-title":"HPB"},{"key":"2025081806221593300_ref7","first-page":"357","volume-title":"Lipids. Lehninger Principles of Biochemistry","author":"Nelson","year":"2013","edition":"6th"},{"key":"2025081806221593300_ref8","doi-asserted-by":"publisher","first-page":"5","DOI":"10.3389\/fphar.2014.00239","article-title":"Inside epoxyeicosatrienoic acids and cardiovascular disease","volume":"5","author":"Tacconelli","year":"2014","journal-title":"Front Pharmacol"},{"key":"2025081806221593300_ref9","doi-asserted-by":"publisher","first-page":"337","DOI":"10.1016\/j.fshw.2019.11.002","article-title":"Cytochrome P450 monooxygenase-mediated eicosanoid pathway: a potential mechanistic linkage between dietary fatty acid consumption and colon cancer","volume":"8","author":"Wang","year":"2019","journal-title":"Food Sci Human Wellness"},{"key":"2025081806221593300_ref10","doi-asserted-by":"publisher","first-page":"1066","DOI":"10.1007\/S00125-017-4260-0","article-title":"Cytochrome P450 epoxygenase-derived epoxyeicosatrienoic acids contribute to insulin sensitivity in mice and in humans","volume":"60","author":"Gangadhariah","year":"2017","journal-title":"Diabetologia"},{"key":"2025081806221593300_ref11","doi-asserted-by":"publisher","first-page":"103279","DOI":"10.1016\/j.ebiom.2021.103279","article-title":"Plasma epoxyeicosatrienoic acids and dihydroxyeicosatrieonic acids, insulin, glucose and risk of diabetes: the strong heart study","volume":"66","author":"Lemaitre","year":"2021","journal-title":"EBioMedicine"},{"key":"2025081806221593300_ref12","doi-asserted-by":"publisher","first-page":"997","DOI":"10.2337\/db09-1241","article-title":"Increased CYP2J3 expression reduces insulin resistance in fructose-treated rats and db\/db mice","volume":"59","author":"Xu","year":"2010","journal-title":"Diabetes"},{"key":"2025081806221593300_ref13","doi-asserted-by":"publisher","first-page":"451","DOI":"10.1124\/jpet.111.180505","article-title":"Epoxyeicosatrienoic acids attenuate reactive oxygen species level, mitochondrial dysfunction, caspase activation, and apoptosis in carcinoma cells treated with arsenic trioxide","volume":"339","author":"Liu","year":"2011","journal-title":"J Pharmacol Exp Ther"},{"key":"2025081806221593300_ref14","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1016\/bs.apha.2016.04.003","article-title":"Epoxyeicosatrienoic acids and 20-hydroxyeicosatetraenoic acid on endothelial and vascular function","volume":"77","author":"Imig","year":"2016","journal-title":"Adv Pharmacol"},{"key":"2025081806221593300_ref15","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1186\/S12933-019-0843-Z","article-title":"Altered bioavailability of epoxyeicosatrienoic acids is associated with conduit artery endothelial dysfunction in type 2 diabetic patients","volume":"18","author":"Duflot","year":"2019","journal-title":"Cardiovasc Diabetol"},{"key":"2025081806221593300_ref16","doi-asserted-by":"publisher","first-page":"C996","DOI":"10.1152\/ajpcell.00402.2006","article-title":"Action of epoxyeicosatrienoic acids on cellular function","volume":"292","author":"Spector","year":"2007","journal-title":"Am J Physiol Cell Physiol"},{"key":"2025081806221593300_ref17","doi-asserted-by":"publisher","first-page":"162","DOI":"10.1016\/J.CHEMPHYSLIP.2018.09.004","article-title":"A sensitive and improved throughput UPLC\u2013MS\/MS quantitation method of total cytochrome P450 mediated arachidonic acid metabolites that can separate regio-isomers and cis\/trans-EETs from human plasma","volume":"216","author":"Zeigler","year":"2018","journal-title":"Chem Phys Lipids"},{"key":"2025081806221593300_ref18","doi-asserted-by":"publisher","first-page":"1320","DOI":"10.1016\/0006-291X(92)91877-S","article-title":"Arachidonic acid epoxygenase: structural characterization and quantification of epoxyeicosatrienoates in plasma","volume":"182","author":"Karara","year":"1992","journal-title":"Biochem Biophys Res Commun"},{"key":"2025081806221593300_ref19","doi-asserted-by":"publisher","first-page":"403","DOI":"10.1007\/s11883-009-0061-3","article-title":"Impact of circulating esterified eicosanoids and other oxylipins on endothelial function","volume":"11","author":"Shearer","year":"2009","journal-title":"Curr Atheroscler Rep"},{"key":"2025081806221593300_ref20","doi-asserted-by":"publisher","first-page":"104189","DOI":"10.1016\/j.ebiom.2022.104189","article-title":"Plasma epoxyeicosatrienoic acids and diabetes-related cardiovascular disease: the cardiovascular health study","volume":"83","author":"Lemaitre","year":"2022","journal-title":"EbioMedicine"},{"key":"2025081806221593300_ref21","doi-asserted-by":"publisher","first-page":"36592","DOI":"10.1074\/JBC.M806315200","article-title":"Opposite regulation of cholesterol levels by the phosphatase and hydrolase domains of soluble epoxide hydrolase","volume":"283","author":"Enayetallah","year":"2008","journal-title":"J Biol Chem"},{"key":"2025081806221593300_ref22","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1146\/ANNUREV-PHARMTOX-011112-140244","article-title":"Impact of soluble epoxide hydrolase and epoxyeicosanoids on human health","volume":"53","author":"Morisseau","year":"2013","journal-title":"Annu Rev Pharmacol Toxicol"},{"key":"2025081806221593300_ref23","doi-asserted-by":"publisher","first-page":"174","DOI":"10.1007\/S11883-010-0108-5","article-title":"Soluble epoxide hydrolase in atherosclerosis","volume":"12","author":"Wang","year":"2010","journal-title":"Curr Atheroscler Rep"},{"key":"2025081806221593300_ref24","doi-asserted-by":"publisher","first-page":"1265","DOI":"10.1161\/ATVBAHA.109.186064","article-title":"Inhibition of soluble epoxide hydrolase attenuated atherosclerosis, abdominal aortic aneurysm formation, and dyslipidemia","volume":"29","author":"Zhang","year":"2009","journal-title":"Arterioscler Thromb Vasc Biol"},{"key":"2025081806221593300_ref25","doi-asserted-by":"publisher","first-page":"233","DOI":"10.1097\/00004836-200203000-00007","article-title":"Magnetic resonance cholangiography versus ultrasound in the evaluation of the gallbladder","volume":"34","author":"Calvo","year":"2002","journal-title":"J Clin Gastroenterol"},{"key":"2025081806221593300_ref26","volume-title":"Obesity and Overweight","author":"World Health Organization"},{"key":"2025081806221593300_ref27","doi-asserted-by":"publisher","first-page":"499","DOI":"10.1093\/CLINCHEM\/18.6.499","article-title":"Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge","volume":"18","author":"Friedewald","year":"1972","journal-title":"Clin Chem"},{"key":"2025081806221593300_ref28","doi-asserted-by":"publisher","first-page":"911","DOI":"10.1139\/y59-099","article-title":"A rapid method of total lipid extraction and purification","volume":"37","author":"Bligh","year":"1959","journal-title":"Can J Biochem Physiol"},{"key":"2025081806221593300_ref29","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1097\/MOG.0000000000000423","article-title":"An update on the pathogenesis of cholesterol gallstone disease","volume":"34","author":"Ciaula","year":"2018","journal-title":"Curr Opin Gastroenterol"},{"key":"2025081806221593300_ref30","doi-asserted-by":"publisher","first-page":"1350","DOI":"10.1136\/bmj.289.6455.1350","article-title":"Weight gain after cholecystectomy","volume":"289","author":"Houghton","year":"1984","journal-title":"Br Med J"},{"key":"2025081806221593300_ref31","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1371\/JOURNAL.PONE.0088189","article-title":"Association of cholecystectomy with metabolic syndrome in a Chinese population","volume":"9","author":"Shen","year":"2014","journal-title":"PLoS One"},{"key":"2025081806221593300_ref32","doi-asserted-by":"publisher","first-page":"138","DOI":"10.1016\/J.JPHS.2018.09.010","article-title":"Improved endogenous epoxyeicosatrienoic acid production mends heart function via increased PGC 1\u03b1-mitochondrial functions in metabolic syndrome","volume":"138","author":"Liu","year":"2018","journal-title":"J Pharmacol Sci"},{"key":"2025081806221593300_ref33","doi-asserted-by":"publisher","first-page":"e6729","DOI":"10.7759\/cureus.6729","article-title":"Is cholecystectomy in patients with symptomatic uncomplicated cholelithiasis beneficial in improving the lipid profile?","volume":"12","author":"Osman","year":"2020","journal-title":"Cureus"},{"key":"2025081806221593300_ref34","doi-asserted-by":"publisher","DOI":"10.21203\/rs.3.rs-2692497\/v1","article-title":"Clinical correlation with metabolic changes post cholecystectomy","author":"Farrugia","year":"2023"},{"key":"2025081806221593300_ref35","doi-asserted-by":"publisher","first-page":"52","DOI":"10.1111\/J.1478-3231.2010.02361.X","article-title":"Cholecystectomy increases hepatic triglyceride content and very-low-density lipoproteins production in mice","volume":"31","author":"Amigo","year":"2011","journal-title":"Liver Int"},{"key":"2025081806221593300_ref36","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1016\/S1665-2681(19)31490-5","article-title":"Association between cholecystectomy for gallstone disease and risk factors for cardiovascular disease","volume":"11","author":"Chavez-Tapia","year":"2012","journal-title":"Ann Hepatol"},{"key":"2025081806221593300_ref37","doi-asserted-by":"publisher","first-page":"151","DOI":"10.4103\/mj.mj_45_22","article-title":"The effect of laparoscopic cholecystectomy on lipid profile","volume":"21","author":"Shuwelif","year":"2022","journal-title":"Mustansiriya Med J"},{"key":"2025081806221593300_ref38","doi-asserted-by":"publisher","first-page":"5935","DOI":"10.3390\/ijms23115935","article-title":"Effects of bile acid modulation by dietary fat, cholecystectomy, and bile acid sequestrant on energy, glucose, and lipid metabolism and gut microbiota in mice","volume":"23","author":"Park","year":"2022","journal-title":"Int J Mol Sci"},{"key":"2025081806221593300_ref39","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1016\/j.apsb.2015.01.004","article-title":"Bile acid nuclear receptor FXR and digestive system diseases","volume":"5","author":"Ding","year":"2015","journal-title":"Acta Pharm Sin B"},{"key":"2025081806221593300_ref40","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1038\/LABINVEST.2017.129","article-title":"Cholecystectomy: a way forward and back to metabolic syndrome?","volume":"98","author":"Garruti","year":"2018","journal-title":"Lab Investig"},{"key":"2025081806221593300_ref41","doi-asserted-by":"publisher","first-page":"2232","DOI":"10.1053\/j.gastro.2020.08.001","article-title":"Epoxygenase-derived epoxyeicosatrienoic acid mediators are associated with nonalcoholic fatty liver disease, nonalcoholic steatohepatitis, and fibrosis","volume":"159","author":"Arvind","year":"2020","journal-title":"Gastroenterology"}],"container-title":["Postgraduate Medical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/pmj\/article-pdf\/101\/1199\/828\/61700064\/qgae175.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/pmj\/article-pdf\/101\/1199\/828\/61700064\/qgae175.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,18]],"date-time":"2025-08-18T10:22:26Z","timestamp":1755512546000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/pmj\/article\/101\/1199\/828\/7990525"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,30]]},"references-count":41,"journal-issue":{"issue":"1199","published-online":{"date-parts":[[2025,1,30]]},"published-print":{"date-parts":[[2025,8,17]]}},"URL":"https:\/\/doi.org\/10.1093\/postmj\/qgae175","relation":{},"ISSN":["0032-5473","1469-0756"],"issn-type":[{"value":"0032-5473","type":"print"},{"value":"1469-0756","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2025,9]]},"published":{"date-parts":[[2025,1,30]]}}}