{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T18:43:24Z","timestamp":1789757004418,"version":"4.0.1"},"reference-count":48,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2025,11,10]],"date-time":"2025-11-10T00:00:00Z","timestamp":1762732800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2024YFA1106900"],"award-info":[{"award-number":["2024YFA1106900"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFA1100100"],"award-info":[{"award-number":["2021YFA1100100"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Beijing Outstanding Young Scientist Program","award":["JWZQ20240101003"],"award-info":[{"award-number":["JWZQ20240101003"]}]},{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["Z230011"],"award-info":[{"award-number":["Z230011"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32090012"],"award-info":[{"award-number":["32090012"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32225017"],"award-info":[{"award-number":["32225017"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2025T180736"],"award-info":[{"award-number":["2025T180736"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Yifu Outstanding Postdoctoral Award"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The trophectoderm produced from totipotent blastomeres initiates trophoblast development, while placental deficiencies can cause pregnancy disorders. Yet, a culture system that fully recapitulates the entire placenta development is still lacking, greatly limiting related studies. Here, we captured mouse trophectoderm-like stem cells (TELSCs), which can give rise to all trophoblast lineages and can be applied to generate trophoblast organoids. We achieved the induction and maintenance of TELSCs from totipotent blastomere-like stem cells or early embryos through a Hippo-YAP\/Notch-to-TGF\u03b21 signaling switch. At the molecular level, TELSCs resemble E4.5 trophectoderm and are distinct from all previously known trophoblast-like stem cells. Functionally, TELSCs can generate all trophoblast lineages in both teratoma and chimera assays. We further applied TELSCs to generate trophoblast organoids containing various mature trophoblasts and a self-renewing extraembryonic ectoderm (ExE)-like progenitor population. Interestingly, we observed transiently formed rosette-like structures that rely on Itgb1, which are essential to induce ExE-like progenitors and to generate organoids eventually. Thus, the capture of TELSCs enables comprehensive insights into placental development.<\/jats:p>","DOI":"10.1093\/procel\/pwaf098","type":"journal-article","created":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T13:02:34Z","timestamp":1762520554000},"page":"339-356","source":"Crossref","is-referenced-by-count":1,"title":["Capturing trophectoderm-like stem cells enables step-wisely remodeling of placental development"],"prefix":"10.1093","volume":"17","author":[{"given":"Xinyi","family":"Jia","sequence":"first","affiliation":[{"name":"MOE Key Laboratory of Cell Proliferation and Differentiation, School of Life Sciences, Peking University , Beijing 100871,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-4326-1467","authenticated-orcid":false,"given":"Bing","family":"Peng","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory of Cell Proliferation and Differentiation, School of Life Sciences, Peking University , Beijing 100871,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongjin","family":"Zhao","sequence":"additional","affiliation":[{"name":"Shandong Provincial Hospital Affiliated to Shandong First Medical University , Jinan 250021,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunhui","family":"Wang","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory of Cell Proliferation and Differentiation, School of Life Sciences, Peking University , Beijing 100871,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aibin","family":"He","sequence":"additional","affiliation":[{"name":"Institute of Molecular Medicine and National Biomedical Imaging Center, College of Future Technology, Peking-Tsinghua Center for Life Sciences and State Key Laboratory of Gene Function and Modulation Research, Peking University , Beijing 100871,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1860-895X","authenticated-orcid":false,"given":"Wei","family":"Tao","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory of Cell Proliferation and Differentiation, School of Life Sciences, Peking University , Beijing 100871,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-3768-1758","authenticated-orcid":false,"given":"Peng","family":"Du","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory of Cell Proliferation and Differentiation, School of Life Sciences, Peking University , Beijing 100871,","place":["China"]},{"name":"Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University , Beijing 100871,","place":["China"]},{"name":"Beijing Advanced Center of RNA Biology, Peking University , Beijing 100871,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,11,10]]},"reference":[{"key":"2026042408413173900_pwaf098-B2","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1038\/s41467-023-36019-9","article-title":"Mechanisms and function of de novo DNA methylation in placental development reveals an essential role for DNMT3B","volume":"14","author":"Andrews","year":"2023","journal-title":"Nat Commun"},{"key":"2026042408413173900_pwaf098-B3","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1038\/s41574-020-0372-6","article-title":"Tracking placental development in health and disease","volume":"16","author":"Aplin","year":"2020","journal-title":"Nat Rev Endocrinol"},{"key":"2026042408413173900_pwaf098-B4","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1242\/jcs.175943","article-title":"Embryo-epithelium interactions during implantation at a glance","volume":"130","author":"Aplin","year":"2017","journal-title":"J Cell Sci"},{"key":"2026042408413173900_pwaf098-B5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.placenta.2017.12.002","article-title":"Sirtuin1 is required for proper trophoblast differentiation and placental development in mice","volume":"62","author":"Arul Nambi Rajan","year":"2018","journal-title":"Placenta"},{"key":"2026042408413173900_pwaf098-B6","doi-asserted-by":"crossref","first-page":"1032","DOI":"10.1016\/j.cell.2014.01.023","article-title":"Self-organizing properties of mouse pluripotent cells initiate morphogenesis upon implantation","volume":"156","author":"Bedzhov","year":"2014","journal-title":"Cell"},{"key":"2026042408413173900_pwaf098-B8","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1242\/dev.128314","article-title":"Lineage specification in the mouse preimplantation 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