{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T19:14:15Z","timestamp":1788462855113,"version":"build-2803163510"},"reference-count":0,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Sepsis is systemic inflammation with high mortality, accompanied by multi-organ failure including acute respiratory distress syndrome. Extracellular vesicles (EVs) encapsulating bioactive cargoes, mediate cell-cell communication to exert systemic regulation. However, whether and how host lung tissue responds quickly to plasma bacterial infection through EVs in sepsis is poorly understood. Here, we identify that peripheral blood macrophages secrete more exosomal G6PD protein to induce lung injury by rewiring purine metabolism during sepsis. Guanine accumulation reduces H3K27 trimethylation and subsequently induces Nos2, Ccl6 and Il6 expression by suppressing de novo EZH2 synthesis. Macrophage-specific Rab27a or G6pd knockout mice had low exosomal G6PD protein in serum, and failed to exert lung injury upon bacterial infection. Beyond, targeting macrophage-derived G6PD by G6PD inhibitors or engineered EVs delivering si-G6pd relieves lung injury in septic mice. In summary, our findings reveal that circulating G6PD promotes lung injury by rewiring purine metabolism and remodeling the epigenetic profile in sepsis, shedding light on the critical role of EVs as a pro-inflammatory signal and targeting G6PD for future sepsis diagnosis and treatment.<\/jats:p>","DOI":"10.1093\/procel\/pwag065","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T18:32:12Z","timestamp":1788460332000},"source":"Crossref","is-referenced-by-count":0,"title":["Guanine promotes acute lung injury by repressing H3K27me3 in sepsis"],"prefix":"10.1093","author":[{"given":"Chao-Qun","family":"Wang","sequence":"first","affiliation":[{"name":"Wuhan University State Key Laboratory of Metabolism and Regulation in Complex Organisms, Hubei Provincial Research Center for Basic Biological Sciences, College of Life Sciences, TaiKang Center for Life and Medical Sciences, , Wuhan 430072,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui-Juan","family":"Wang","sequence":"additional","affiliation":[{"name":"Renmin Hospital of Wuhan University Department of Critical Care Medicine, , Wuhan 430060,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue-Yu","family":"Kong","sequence":"additional","affiliation":[{"name":"Wuhan University Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, TaiKang Center for Life and Medical Sciences, Frontier Science Center for Immunology and Metabolism, , Wuhan 430072,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiayila","family":"Abulaiti","sequence":"additional","affiliation":[{"name":"Wuhan University State Key Laboratory of Metabolism and Regulation in Complex Organisms, Hubei Provincial Research Center for Basic Biological Sciences, College of Life Sciences, TaiKang Center for Life and Medical Sciences, , Wuhan 430072,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Hui","family":"Zhang","sequence":"additional","affiliation":[{"name":"Wuhan University State Key Laboratory of Metabolism and Regulation in Complex Organisms, Hubei Provincial Research Center for Basic Biological Sciences, College of Life Sciences, TaiKang Center for Life and Medical Sciences, , Wuhan 430072,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liu","family":"Liu","sequence":"additional","affiliation":[{"name":"Wuhan University College of Chemistry and Molecular Sciences, TaiKang Center for Life and Medical Sciences, , Wuhan 430072, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui-Feng","family":"Shi","sequence":"additional","affiliation":[{"name":"Wuhan University State Key Laboratory of Metabolism and Regulation in Complex Organisms, Hubei Provincial Research Center for Basic Biological Sciences, College of Life Sciences, TaiKang Center for Life and Medical Sciences, , Wuhan 430072,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng","family":"Hu","sequence":"additional","affiliation":[{"name":"Wuhan University State Key Laboratory of Metabolism and Regulation in Complex Organisms, Hubei Provincial Research Center for Basic Biological Sciences, College of Life Sciences, TaiKang Center for Life and Medical Sciences, , Wuhan 430072,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu","family":"Li","sequence":"additional","affiliation":[{"name":"Wuhan University State Key Laboratory of Metabolism and Regulation in Complex Organisms, Hubei Provincial Research Center for Basic Biological Sciences, College of Life Sciences, TaiKang Center for Life and Medical Sciences, , Wuhan 430072,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei-Xin","family":"Li","sequence":"additional","affiliation":[{"name":"Wuhan University State Key Laboratory of Metabolism and Regulation in Complex Organisms, Hubei Provincial Research Center for Basic Biological Sciences, College of Life Sciences, TaiKang Center for Life and Medical Sciences, , Wuhan 430072,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Wang","sequence":"additional","affiliation":[{"name":"Renmin Hospital of Wuhan University Department of Critical Care Medicine, , Wuhan 430060,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia-Xi","family":"Lei","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Xi\u2019an Jiaotong University Department of Surgical Intensive Care Unit, , Xi\u2019an 710061,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Capital Medical University Department of Anesthesiology, Beijing Anzhen Hospital, , Beijing 100029,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Min","family":"Xiang","sequence":"additional","affiliation":[{"name":"Wuhan University College of Chemistry and Molecular Sciences, TaiKang Center for Life and Medical Sciences, , Wuhan 430072,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Long-Fei","family":"Wang","sequence":"additional","affiliation":[{"name":"Wuhan University School of Pharmaceutical Sciences, , Wuhan 430072,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Long","sequence":"additional","affiliation":[{"name":"Wuhan University School of Basic Medical Sciences, , Wuhan 430071,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8062-5411","authenticated-orcid":false,"given":"Hai-Ning","family":"Du","sequence":"additional","affiliation":[{"name":"Wuhan University Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, TaiKang Center for Life and Medical Sciences, Frontier Science Center for Immunology and Metabolism, , Wuhan 430072,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1667-0570","authenticated-orcid":false,"given":"Li-Ying","family":"Zhan","sequence":"additional","affiliation":[{"name":"Renmin Hospital of Wuhan University Department of Critical Care Medicine, , Wuhan 430060,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3978-7675","authenticated-orcid":false,"given":"Wei","family":"Yan","sequence":"additional","affiliation":[{"name":"Wuhan University State Key Laboratory of Metabolism and Regulation in Complex Organisms, Hubei Provincial Research Center for Basic Biological Sciences, College of Life Sciences, TaiKang Center for Life and Medical Sciences, , Wuhan 430072,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,9,3]]},"container-title":["Protein &amp; 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