{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T20:30:23Z","timestamp":1786998623674,"version":"build-2736575974"},"reference-count":63,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T00:00:00Z","timestamp":1784246400000},"content-version":"vor","delay-in-days":197,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"NIH National Institute of General Medical Sciences Maximizing Investigators\u2019 Research Award","award":["R35GM147104"],"award-info":[{"award-number":["R35GM147104"]}]},{"name":"University of South Florida New Researcher"},{"DOI":"10.13039\/100008900","name":"University of South Florida","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008900","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,1,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Engineering proteins for therapeutic applications is a field that has seen substantial growth in the past two decades, but challenges remain. A major deficiency in current strategies is the lack of a means to efficiently screen for binding to key epitopes to yield a desired function. To meet this challenge, we designed a tethered yeast surface display construct that leverages high local concentrations by tethering a candidate protein binder to a target protein of interest (POI) via a flexible peptide linker. These high local concentrations enable screening of epitope-specific binders based on decreased signal due to inhibitory function when the construct is assayed alongside a competitive POI binder. We demonstrate that epitope-specific screening and enrichment is possible based on fluorescent output and study key optimization parameters. We anticipate this technique will accelerate binder development by reducing the need for downstream low throughput epitope mapping of isolated proteins.<\/jats:p>","DOI":"10.1093\/protein\/gzag019","type":"journal-article","created":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T11:44:17Z","timestamp":1784202257000},"source":"Crossref","is-referenced-by-count":0,"title":["A tethered yeast surface display system facilitates enriching epitope-specific binding interactions"],"prefix":"10.1093","volume":"39","author":[{"given":"Rojhae A","family":"Panton","sequence":"first","affiliation":[{"name":"Department of Chemical, Biological, and Materials Engineering, University of South Florida , Tampa, FL 33620 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jakob","family":"Lind","sequence":"additional","affiliation":[{"name":"Department of Chemical, Biological, and Materials Engineering, University of South Florida , Tampa, FL 33620 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda","family":"Richardson","sequence":"additional","affiliation":[{"name":"Department of Chemical, Biological, and Materials Engineering, University of South Florida , Tampa, FL 33620 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabela","family":"Panozzo","sequence":"additional","affiliation":[{"name":"Department of Chemical, Biological, and Materials Engineering, University of South Florida , Tampa, FL 33620 ,","place":["United States"]},{"name":"Department of Medical Engineering, University of South Florida , Tampa, FL 33620 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Gomez Garcia","sequence":"additional","affiliation":[{"name":"Department of Chemical, Biological, and Materials Engineering, University of South Florida , Tampa, FL 33620 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Syed","family":"Muzaffar","sequence":"additional","affiliation":[{"name":"Department of Chemical, Biological, and Materials Engineering, University of South Florida , Tampa, FL 33620 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3369-7495","authenticated-orcid":false,"given":"Lawrence A","family":"Stern","sequence":"additional","affiliation":[{"name":"Department of Chemical, Biological, and Materials Engineering, University of South Florida , Tampa, FL 33620 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,7,17]]},"reference":[{"key":"2026081715264224900_ref1","doi-asserted-by":"publisher","first-page":"493","DOI":"10.1038\/s41586-024-07487-w","article-title":"Accurate structure prediction of biomolecular interactions with AlphaFold 3","volume":"630","author":"Abramson","year":"2024","journal-title":"Nature"},{"key":"2026081715264224900_ref2","doi-asserted-by":"publisher","first-page":"9396","DOI":"10.1021\/ja025870q","article-title":"In vitro selection for catalytic activity with ribosome display","volume":"124","author":"Amstutz","year":"2002","journal-title":"J Am Chem Soc"},{"key":"2026081715264224900_ref3","doi-asserted-by":"publisher","first-page":"738","DOI":"10.1021\/acscombsci.0c00171","article-title":"Use of target-displaying magnetized yeast in screening mRNA-display peptide libraries to identify ligands","volume":"22","author":"Bacon","year":"2020","journal-title":"ACS Comb Sci"},{"key":"2026081715264224900_ref4","doi-asserted-by":"publisher","DOI":"10.1093\/protein\/gzaf006","article-title":"A facile yeast-display approach for antibody mask discovery","volume":"38","author":"Badarinath","year":"2025","journal-title":"Protein Eng Des Sel"},{"key":"2026081715264224900_ref5","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1007\/BF00397923","article-title":"The occurrence of epidermal growth factor receptors and the characterization of EGF-like factors in human ovarian, endometrial, cervical and breast cancer. EGF receptors and factors in gynecological carcinomas","volume":"115","author":"Bauknecht","year":"1989","journal-title":"J Cancer Res Clin Oncol"},{"key":"2026081715264224900_ref6","doi-asserted-by":"publisher","first-page":"423","DOI":"10.1126\/science.3140379","article-title":"Single-chain antigen-binding proteins","volume":"242","author":"Bird","year":"1988","journal-title":"Science"},{"key":"2026081715264224900_ref7","doi-asserted-by":"publisher","first-page":"553","DOI":"10.1038\/nbt0697-553","article-title":"Yeast surface display for screening combinatorial polypeptide libraries","volume":"15","author":"Boder","year":"1997","journal-title":"Nat Biotechnol"},{"key":"2026081715264224900_ref8","doi-asserted-by":"publisher","first-page":"485","DOI":"10.1002\/bit.20616","article-title":"Yeast surface display of a noncovalent MHC class II heterodimer complexed with antigenic peptide","volume":"92","author":"Boder","year":"2005","journal-title":"Biotechnol Bioeng"},{"key":"2026081715264224900_ref9","doi-asserted-by":"publisher","first-page":"539","DOI":"10.1016\/j.jmb.2004.07.053","article-title":"Fine epitope mapping of anti-epidermal growth factor receptor antibodies through random mutagenesis and yeast surface display","volume":"342","author":"Chao","year":"2004","journal-title":"J Mol Biol"},{"key":"2026081715264224900_ref10","doi-asserted-by":"publisher","first-page":"1357","DOI":"10.1016\/j.addr.2012.09.039","article-title":"Fusion protein linkers: Property, design and functionality","volume":"65","author":"Chen","year":"2013","journal-title":"Adv Drug Deliv Rev"},{"key":"2026081715264224900_ref11","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1007\/978-1-4939-2748-7_8","article-title":"Applications of yeast surface display for protein engineering","volume":"1319","author":"Cherf","year":"2015","journal-title":"Methods Mol Biol"},{"key":"2026081715264224900_ref12","doi-asserted-by":"publisher","first-page":"717","DOI":"10.1208\/s12248-013-9477-3","article-title":"Factors influencing magnitude and duration of target inhibition following antibody therapy: Implications in drug discovery and development","volume":"15","author":"Chimalakonda","year":"2013","journal-title":"AAPS J"},{"key":"2026081715264224900_ref13","doi-asserted-by":"publisher","first-page":"147","DOI":"10.1016\/j.jim.2004.01.024","article-title":"Domain-level antibody epitope mapping through yeast surface display of epidermal growth factor receptor fragments","volume":"287","author":"Cochran","year":"2004","journal-title":"J Immunol Methods"},{"key":"2026081715264224900_ref14","doi-asserted-by":"publisher","first-page":"399","DOI":"10.1016\/j.cbpa.2004.06.010","article-title":"Making drugs on proteins: Site-directed ligand discovery for fragment-based lead assembly","volume":"8","author":"Erlanson","year":"2004","journal-title":"Curr Opin Chem Biol"},{"key":"2026081715264224900_ref15","doi-asserted-by":"publisher","first-page":"9367","DOI":"10.1073\/pnas.97.17.9367","article-title":"Site-directed ligand discovery","volume":"97","author":"Erlanson","year":"2000","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2026081715264224900_ref16","doi-asserted-by":"publisher","first-page":"275","DOI":"10.1007\/978-1-0716-3279-6_15","article-title":"Tyrosine phosphorylation screening on the yeast surface by magnetic bead selection and FACS","volume":"2681","author":"Ezagui","year":"2023","journal-title":"Methods Mol Biol"},{"key":"2026081715264224900_ref17","doi-asserted-by":"publisher","first-page":"233","DOI":"10.1093\/bib\/bbp021","article-title":"Progress and challenges in predicting protein-protein interaction sites","volume":"10","author":"Ezkurdia","year":"2009","journal-title":"Brief Bioinform"},{"key":"2026081715264224900_ref18","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1038\/nbt785","article-title":"Flow-cytometric isolation of human antibodies from a nonimmune Saccharomyces cerevisiae surface display library","volume":"21","author":"Feldhaus","year":"2003","journal-title":"Nat Biotechnol"},{"key":"2026081715264224900_ref19","doi-asserted-by":"publisher","first-page":"955","DOI":"10.1007\/s00109-005-0705-x","article-title":"Targeting protein-protein interactions for cancer therapy","volume":"83","author":"Fry","year":"2005","journal-title":"J Mol Med (Berl)"},{"key":"2026081715264224900_ref20","doi-asserted-by":"publisher","first-page":"467","DOI":"10.1016\/j.sbi.2007.08.012","article-title":"Yeast surface display for protein engineering and characterization","volume":"17","author":"Gai","year":"2007","journal-title":"Curr Opin Struct Biol"},{"key":"2026081715264224900_ref21","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1016\/j.ymeth.2012.03.014","article-title":"Protein selection using yeast surface display","volume":"60","author":"Gera","year":"2013","journal-title":"Methods"},{"key":"2026081715264224900_ref22","doi-asserted-by":"publisher","first-page":"1238","DOI":"10.1016\/j.jmb.2008.06.051","article-title":"Picomolar affinity fibronectin domains engineered utilizing loop length diversity, recursive mutagenesis, and loop shuffling","volume":"381","author":"Hackel","year":"2008","journal-title":"J Mol Biol"},{"key":"2026081715264224900_ref23","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1093\/protein\/gzr056","article-title":"Epidermal growth factor receptor downregulation by small heterodimeric binding proteins","volume":"25","author":"Hackel","year":"2012","journal-title":"Protein Eng Des Sel"},{"key":"2026081715264224900_ref24","doi-asserted-by":"publisher","first-page":"114","DOI":"10.1016\/j.coph.2018.05.010","article-title":"Monoclonal antibodies as immunomodulatory therapy against cancer and autoimmune diseases","volume":"41","author":"Hafeez","year":"2018","journal-title":"Curr Opin Pharmacol"},{"key":"2026081715264224900_ref25","doi-asserted-by":"publisher","first-page":"2239","DOI":"10.1016\/j.bmcl.2018.05.048","article-title":"'Tethering' fragment-based drug discovery to identify inhibitors of the essential respiratory membrane protein type II NADH dehydrogenase","volume":"28","author":"Heikal","year":"2018","journal-title":"Bioorg Med Chem Lett"},{"key":"2026081715264224900_ref26","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1016\/j.tips.2012.03.006","article-title":"Structural biology and drug discovery for protein-protein interactions","volume":"33","author":"Jubb","year":"2012","journal-title":"Trends Pharmacol Sci"},{"key":"2026081715264224900_ref28","doi-asserted-by":"publisher","first-page":"4940","DOI":"10.1158\/0008-5472.CAN-07-0283","article-title":"The antibody-mediated targeted delivery of interleukin-15 and GM-CSF to the tumor neovasculature inhibits tumor growth and metastasis","volume":"67","author":"Kaspar","year":"2007","journal-title":"Cancer Res"},{"key":"2026081715264224900_ref29","doi-asserted-by":"publisher","first-page":"715","DOI":"10.1038\/35070613","article-title":"Functional proteins from a random-sequence library","volume":"410","author":"Keefe","year":"2001","journal-title":"Nature"},{"key":"2026081715264224900_ref30","doi-asserted-by":"publisher","first-page":"1026","DOI":"10.1002\/prot.20618","article-title":"Directed evolution of the epidermal growth factor receptor extracellular domain for expression in yeast","volume":"62","author":"Kim","year":"2006","journal-title":"Proteins"},{"key":"2026081715264224900_ref31","doi-asserted-by":"publisher","first-page":"90","DOI":"10.1016\/j.jbiotec.2015.02.008","article-title":"Effect of linker flexibility and length on the functionality of a cytotoxic engineered antibody fragment","volume":"199","author":"Klement","year":"2015","journal-title":"J Biotechnol"},{"key":"2026081715264224900_ref32","doi-asserted-by":"publisher","first-page":"194","DOI":"10.1016\/j.abb.2012.03.001","article-title":"Antibody-cytokine fusion proteins","volume":"526","author":"Kontermann","year":"2012","journal-title":"Arch Biochem Biophys"},{"key":"2026081715264224900_ref33","doi-asserted-by":"publisher","first-page":"2043","DOI":"10.1093\/emboj\/16.8.2043","article-title":"Linker length and composition influence the flexibility of Oct-1 DNA binding","volume":"16","author":"Leeuwen","year":"1997","journal-title":"EMBO J"},{"key":"2026081715264224900_ref34","doi-asserted-by":"publisher","first-page":"196","DOI":"10.1016\/j.jmb.2006.09.084","article-title":"Fine and domain-level epitope mapping of botulinum neurotoxin type a neutralizing antibodies by yeast surface display","volume":"365","author":"Levy","year":"2007","journal-title":"J Mol Biol"},{"key":"2026081715264224900_ref35","doi-asserted-by":"publisher","DOI":"10.1002\/biot.201600696","article-title":"Dual display of proteins on the yeast cell surface simplifies quantification of binding interactions and enzymatic bioconjugation reactions","volume":"12","author":"Lim","year":"2017","journal-title":"Biotechnol J"},{"key":"2026081715264224900_ref36","doi-asserted-by":"publisher","first-page":"901","DOI":"10.3892\/ol.2015.3277","article-title":"Epidermal growth factor receptor protein overexpression and gene amplification are associated with aggressive biological behaviors of esophageal squamous cell carcinoma","volume":"10","author":"Lin","year":"2015","journal-title":"Oncol Lett"},{"key":"2026081715264224900_ref37","doi-asserted-by":"publisher","first-page":"1171","DOI":"10.1038\/nbt1336","article-title":"Computational design of antibody-affinity improvement beyond in vivo maturation","volume":"25","author":"Lippow","year":"2007","journal-title":"Nat Biotechnol"},{"key":"2026081715264224900_ref38","doi-asserted-by":"publisher","first-page":"16314","DOI":"10.1073\/pnas.1903330116","article-title":"Functional selection of protease inhibitory antibodies","volume":"116","author":"Lopez","year":"2019","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2026081715264224900_ref39","doi-asserted-by":"publisher","DOI":"10.1002\/smsc.202300095","article-title":"Protein engineering and high-throughput screening by yeast surface display: Survey of current methods","volume":"3","author":"Lopez-Morales","year":"2023","journal-title":"Small Sci"},{"key":"2026081715264224900_ref40","doi-asserted-by":"publisher","first-page":"903","DOI":"10.1002\/bit.28621","article-title":"Leveraging yeast sequestration to study and engineer posttranslational modification enzymes","volume":"121","author":"Martinusen","year":"2024","journal-title":"Biotechnol Bioeng"},{"key":"2026081715264224900_ref41","doi-asserted-by":"publisher","first-page":"9022","DOI":"10.1073\/pnas.91.19.9022","article-title":"An in vitro polysome display system for identifying ligands from very large peptide libraries","volume":"91","author":"Mattheakis","year":"1994","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2026081715264224900_ref42","doi-asserted-by":"publisher","first-page":"531","DOI":"10.4161\/mabs.1.6.10195","article-title":"Raxibacumab","volume":"1","author":"Mazumdar","year":"2009","journal-title":"MAbs"},{"key":"2026081715264224900_ref43","doi-asserted-by":"publisher","first-page":"422","DOI":"10.4161\/mabs.1.5.9286","article-title":"Golimumab","volume":"1","author":"Mazumdar","year":"2009","journal-title":"MAbs"},{"key":"2026081715264224900_ref44","doi-asserted-by":"publisher","first-page":"13812","DOI":"10.1021\/ja076197d","article-title":"Tethering small molecules to a phage display library: Discovery of a selective bivalent inhibitor of protein kinase a","volume":"129","author":"Meyer","year":"2007","journal-title":"J Am Chem Soc"},{"key":"2026081715264224900_ref45","doi-asserted-by":"publisher","first-page":"395","DOI":"10.1016\/j.str.2016.12.016","article-title":"Mapping protein binding sites and conformational epitopes using cysteine Labeling and yeast surface display","volume":"25","author":"Najar","year":"2017","journal-title":"Structure"},{"key":"2026081715264224900_ref46","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1007\/978-1-0716-2285-8_12","article-title":"Ligand selection by combination of recombinant and cell panning selection techniques","volume":"2491","author":"Panton","year":"2022","journal-title":"Methods Mol Biol"},{"key":"2026081715264224900_ref47","doi-asserted-by":"publisher","first-page":"546","DOI":"10.4103\/0975-7406.90113","article-title":"Ipilimumab: Melanoma and beyond","volume":"3","author":"Patel","year":"2011","journal-title":"J Pharm Bioallied Sci"},{"key":"2026081715264224900_ref48","doi-asserted-by":"publisher","first-page":"707","DOI":"10.1038\/mt.2015.214","article-title":"Modulation of protein-protein interactions for the development of novel therapeutics","volume":"24","author":"Petta","year":"2016","journal-title":"Mol Ther"},{"key":"2026081715264224900_ref49","first-page":"4593","article-title":"Humanization of an anti-Vacsular endothelial growth factor monoclonal antibody for the Therpay of solid Tumors and other disorders","volume":"57","author":"Presta","year":"1997","journal-title":"Cancer Res"},{"key":"2026081715264224900_ref50","doi-asserted-by":"publisher","first-page":"1192","DOI":"10.1002\/bit.22338","article-title":"Directed evolution of a secretory leader for the improved expression of heterologous proteins and full-length antibodies in Saccharomyces cerevisiae","volume":"103","author":"Rakestraw","year":"2009","journal-title":"Biotechnol Bioeng"},{"key":"2026081715264224900_ref51","doi-asserted-by":"publisher","first-page":"6434","DOI":"10.1021\/acs.biochem.7b00929","article-title":"Impact of linker length and composition on fragment binding and cell permeation: Story of a Bisbenzimidazole dye fragment","volume":"56","author":"Ranjan","year":"2017","journal-title":"Biochemistry"},{"key":"2026081715264224900_ref52","first-page":"769","article-title":"Palivizumab for the prevention of respiratory syncytial virus infection","volume":"56","author":"Rogovik","year":"2010","journal-title":"Can Fam Physician"},{"key":"2026081715264224900_ref53","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1002\/yea.1431","article-title":"A flexible protein linker improves the function of epitope-tagged proteins in Saccharomyces cerevisiae","volume":"24","author":"Sabourin","year":"2007","journal-title":"Yeast"},{"key":"2026081715264224900_ref54","doi-asserted-by":"publisher","first-page":"489","DOI":"10.1093\/protein\/gzt023","article-title":"PASylation: A biological alternative to PEGylation for extending the plasma half-life of pharmaceutically active proteins","volume":"26","author":"Schlapschy","year":"2013","journal-title":"Protein Eng Des Sel"},{"key":"2026081715264224900_ref55","doi-asserted-by":"publisher","first-page":"3057","DOI":"10.1021\/bm800529b","article-title":"Effects of linker length and flexibility on multivalent targeting","volume":"9","author":"Shewmake","year":"2008","journal-title":"Biomacromolecules"},{"key":"2026081715264224900_ref56","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0163366","article-title":"The Panitumumab EGFR complex reveals a binding mechanism that overcomes Cetuximab induced resistance","volume":"11","author":"Sickmier","year":"2016","journal-title":"PLoS One"},{"key":"2026081715264224900_ref57","doi-asserted-by":"publisher","first-page":"2328","DOI":"10.1002\/bit.26001","article-title":"Geometry and expression enhance enrichment of functional yeast-displayed ligands via cell panning","volume":"113","author":"Stern","year":"2016","journal-title":"Biotechnol Bioeng"},{"key":"2026081715264224900_ref58","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1177\/1758834014567470","article-title":"Nivolumab in NSCLC: Latest evidence and clinical potential","volume":"7","author":"Sundar","year":"2015","journal-title":"Ther Adv Med Oncol"},{"key":"2026081715264224900_ref59","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1007\/s13238-017-0412-8","article-title":"Distinct PD-L1 binding characteristics of therapeutic monoclonal antibody durvalumab","volume":"9","author":"Tan","year":"2018","journal-title":"Protein Cell"},{"key":"2026081715264224900_ref60","doi-asserted-by":"publisher","first-page":"1772","DOI":"10.1016\/j.chembiol.2021.05.022","article-title":"Mapping the residue specificities of epigenome enzymes by yeast surface display","volume":"28","author":"Waldman","year":"2021","journal-title":"Cell Chem Biol"},{"key":"2026081715264224900_ref61","doi-asserted-by":"publisher","first-page":"1540","DOI":"10.1016\/j.bbadis.2015.04.020","article-title":"EGFR over-expression in non-small cell lung cancers harboring EGFR mutations is associated with marked down-regulation of CD82","volume":"1852","author":"Yang","year":"2015","journal-title":"Biochim Biophys Acta"},{"key":"2026081715264224900_ref62","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1186\/s12934-019-1133-x","article-title":"Development of novel surface display platforms for anchoring heterologous proteins in Saccharomyces cerevisiae","volume":"18","author":"Yang","year":"2019","journal-title":"Microb Cell Factories"},{"key":"2026081715264224900_ref63","doi-asserted-by":"publisher","first-page":"417","DOI":"10.1007\/s10529-011-0793-3","article-title":"Fusion of HSA influences TNF-alpha neutralizing activity of shTNFRs","volume":"34","author":"Zhan","year":"2012","journal-title":"Biotechnol Lett"},{"key":"2026081715264224900_ref64","doi-asserted-by":"publisher","first-page":"65","DOI":"10.4155\/fmc.09.12","article-title":"Targeting protein-protein interactions for therapeutic intervention: A challenge for the future","volume":"1","author":"Zinzalla","year":"2009","journal-title":"Future Med Chem"}],"container-title":["Protein Engineering, Design and Selection"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/peds\/advance-article-pdf\/doi\/10.1093\/protein\/gzag019\/69159895\/gzag019.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/peds\/article-pdf\/doi\/10.1093\/protein\/gzag019\/69159895\/gzag019.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/peds\/article-pdf\/doi\/10.1093\/protein\/gzag019\/69159895\/gzag019.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T19:26:49Z","timestamp":1786994809000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/peds\/article\/doi\/10.1093\/protein\/gzag019\/8736498"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":63,"URL":"https:\/\/doi.org\/10.1093\/protein\/gzag019","relation":{},"ISSN":["1741-0126","1741-0134"],"issn-type":[{"value":"1741-0126","type":"print"},{"value":"1741-0134","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026]]},"published":{"date-parts":[[2026]]},"article-number":"gzag019"}}