{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T17:25:24Z","timestamp":1787505924696,"version":"build-2736575974"},"reference-count":34,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2019,7,2]],"date-time":"2019-07-02T00:00:00Z","timestamp":1562025600000},"content-version":"vor","delay-in-days":213,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Tau protein is a microtubule-stabilising protein whose aggregation is linked to Alzheimer\u2019s Disease and other forms of dementia. Tau biology is at the heart of cytoskeletal dynamics and neurodegenerative mechanisms, making it a crucial protein to study. Tau purification, however, is challenging as Tau is disordered, which makes it difficult to produce in recombinant system and is degradation-prone. It is thus challenging to obtain pure and stable preparations of Tau. Here, we present a fast and robust protocol to purify Tau recombinantly in Escherichia coli. Our protocol allows purifying Tau either tag-less or FLAG-tagged at its N-terminus, and Tau fragments of interest. By exploiting a cleavable affinity tag and two anion exchange columns, we obtained Tau preparations of high purity, stable and suitable for in vitro studies, including aggregation experiments that resemble neurodegenerative processes.<\/jats:p>","DOI":"10.1093\/protein\/gzz010","type":"journal-article","created":{"date-parts":[[2019,6,6]],"date-time":"2019-06-06T15:12:34Z","timestamp":1559833954000},"page":"447-455","source":"Crossref","is-referenced-by-count":19,"title":["Recombinant production and purification of the human protein Tau"],"prefix":"10.1093","volume":"31","author":[{"given":"Luca","family":"Ferrari","sequence":"first","affiliation":[{"name":"Cellular Protein Chemistry, Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, Utrecht, The Netherlands"},{"name":"Science for Life, Utrecht University, Padualaan 8, Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1807-2972","authenticated-orcid":false,"given":"Stefan G D","family":"R\u00fcdiger","sequence":"additional","affiliation":[{"name":"Cellular Protein Chemistry, Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, Utrecht, The Netherlands"},{"name":"Science for Life, Utrecht University, Padualaan 8, Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2019,7,2]]},"reference":[{"key":"2019072211401438800_gzz010C1","doi-asserted-by":"crossref","first-page":"2853","DOI":"10.1021\/ja312471h","volume":"135","author":"Akoury","year":"2013","journal-title":"J. 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Sci."}],"container-title":["Protein Engineering, Design and Selection"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/peds\/advance-article-pdf\/doi\/10.1093\/protein\/gzz010\/28897532\/gzz010.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/peds\/article-pdf\/31\/12\/447\/28970974\/gzz010.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,9,19]],"date-time":"2022-09-19T14:57:35Z","timestamp":1663599455000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/peds\/article\/31\/12\/447\/5527340"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,12,1]]},"references-count":34,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2019,7,2]]},"published-print":{"date-parts":[[2018,12,1]]}},"URL":"https:\/\/doi.org\/10.1093\/protein\/gzz010","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/545566","asserted-by":"object"}]},"ISSN":["1741-0126","1741-0134"],"issn-type":[{"value":"1741-0126","type":"print"},{"value":"1741-0134","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2018,12]]},"published":{"date-parts":[[2018,12,1]]}}}