{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T23:26:04Z","timestamp":1773789964542,"version":"3.50.1"},"reference-count":56,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2019,11,26]],"date-time":"2019-11-26T00:00:00Z","timestamp":1574726400000},"content-version":"vor","delay-in-days":298,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["GM078114"],"award-info":[{"award-number":["GM078114"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,12,13]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The polypeptide amylin is responsible for islet amyloid in type 2 diabetes, a process which contributes to \u03b2-cell death in the disease. The role of the N-terminal region of amylin in amyloid formation is relatively unexplored, although removal of the disulfide bridged loop between Cys-2 and Cys-7 accelerates amyloid formation. We examine the des Lys-1 variant of human amylin (h-amylin), a variant which is likely produced in vivo. Lys-1 is a region of high charge density in the h-amylin amyloid fiber. The des Lys-1 polypeptide forms amyloid on the same time scale as wild-type amylin in phosphate buffered saline, but does so more rapidly in Tris. The des Lys-1 variant is somewhat less toxic to cultured INS cells than wild type. The implications for the in vitro mechanism of amyloid formation and for comparative analysis of amyloidogenicity are discussed.<\/jats:p>","DOI":"10.1093\/protein\/gzz036","type":"journal-article","created":{"date-parts":[[2019,11,15]],"date-time":"2019-11-15T23:34:16Z","timestamp":1573860856000},"page":"87-93","source":"Crossref","is-referenced-by-count":12,"title":["Amyloidogenicity and cytotoxicity of des-Lys-1 human amylin provides insight into amylin self-assembly and highlights the difficulties of defining amyloidogenicity"],"prefix":"10.1093","volume":"32","author":[{"given":"Kyung-Hoon","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Chemistry, Stony Brook University, Stony Brook, NY 11790-3400, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Zhyvoloup","sequence":"additional","affiliation":[{"name":"Institute of Structural and Molecular Biology, University College London, Gower Street, London WC1E6BT, UK, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Raleigh","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Stony Brook University, Stony Brook, NY 11790-3400, USA"},{"name":"Institute of Structural and Molecular Biology, University College London, Gower Street, London WC1E6BT, UK, and"},{"name":"Laufer Center for Physical and Quantitative Biology, Stony Brook University, Stony Brook, NY 11790-3400, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2019,11,25]]},"reference":[{"key":"2019122311173285900_ref1","doi-asserted-by":"crossref","first-page":"16284","DOI":"10.1021\/bi051432v","volume":"44","author":"Abedini","year":"2005","journal-title":"Biochemistry"},{"key":"2019122311173285900_ref2","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1021\/ol047480+","volume":"7","author":"Abedini","year":"2005","journal-title":"Org. 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