{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T05:19:32Z","timestamp":1787894372726,"version":"build-2784847793"},"reference-count":63,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2020,3,30]],"date-time":"2020-03-30T00:00:00Z","timestamp":1585526400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004442","name":"National Science Centre, Poland","doi-asserted-by":"publisher","award":["2013\/09\/N\/ST8\/00309"],"award-info":[{"award-number":["2013\/09\/N\/ST8\/00309"]}],"id":[{"id":"10.13039\/501100004442","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005416","name":"Norwegian Research Council","doi-asserted-by":"publisher","award":["228415"],"award-info":[{"award-number":["228415"]}],"id":[{"id":"10.13039\/501100005416","id-type":"DOI","asserted-by":"publisher"}]},{"name":"GoBone German-Polish bilateral project","award":["01DS16010A"],"award-info":[{"award-number":["01DS16010A"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,4,25]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Bone tissue regeneration in critical-size defects is possible after implantation of a 3D scaffold and can be additionally enhanced once the scaffold is enriched with drugs or other factors supporting bone remodelling and healing. Sodium alendronate (Aln), a widely used anti-osteoporosis drug, exhibits strong inhibitory effect on bone resorption performed by osteoclasts. Thus, we propose a new approach for the treatment of bone defects in craniofacial region combining biocompatible titanium dioxide scaffolds and poly(l-lactide-co-glycolide) microparticles (MPs) loaded with Aln. The MPs were effectively attached to the surface of the scaffolds\u2019 pore walls by human recombinant collagen. Drug release from the scaffolds was characterized by initial burst (24\u2009\u00b1\u20096% of the drug released within first 24\u2009h) followed by a sustained release phase (on average 5\u2009\u00b5g of Aln released per day from Day 3 to Day 18). In vitro tests evidenced that Aln at concentrations of 5 and 2.5\u2009\u00b5g\/ml was not cytotoxic for MG-63 osteoblast-like cells (viability between 81\u2009\u00b1\u20096% and 98\u2009\u00b1\u20093% of control), but it prevented RANKL-induced formation of osteoclast-like cells from macrophages derived from peripheral blood mononuclear cells, as shown by reduced fusion capability and decreased tartrate-resistant acid phosphatase 5b activity (56\u2009\u00b1\u20095% reduction in comparison to control after 8\u2009days of culture). Results show that it is feasible to design the scaffolds providing required doses of Aln inhibiting osteoclastogenesis, reducing osteoclast activity, but not affecting osteoblast functions, which may be beneficial in the treatment of critical-size bone tissue defects.<\/jats:p>","DOI":"10.1093\/rb\/rbaa012","type":"journal-article","created":{"date-parts":[[2020,3,30]],"date-time":"2020-03-30T15:36:13Z","timestamp":1585582573000},"source":"Crossref","is-referenced-by-count":9,"title":["Sodium alendronate loaded poly(<scp>l<\/scp>-lactide- <i>co<\/i>-glycolide) microparticles immobilized on ceramic scaffolds for local treatment of bone defects"],"prefix":"10.1093","volume":"8","author":[{"given":"\u0141ucja","family":"Rumian","sequence":"first","affiliation":[{"name":"Faculty of Materials Science and Ceramics, Department of Biomaterials and Composites, AGH University of Science and Technology, Al. A. Mickiewicza 30, Krakow 30-059, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cornelia","family":"Wolf-Brandstetter","sequence":"additional","affiliation":[{"name":"Technische Universit\u00e4t Dresden, Institute of Materials Science, Max Bergmann Center of Biomaterials, Budapester Str. 27, Dresden 01-069, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sina","family":"R\u00f6\u00dfler","sequence":"additional","affiliation":[{"name":"Technische Universit\u00e4t Dresden, Institute of Materials Science, Max Bergmann Center of Biomaterials, Budapester Str. 27, Dresden 01-069, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarzyna","family":"Reczy\u0144ska","sequence":"additional","affiliation":[{"name":"Faculty of Materials Science and Ceramics, Department of Biomaterials and Composites, AGH University of Science and Technology, Al. A. Mickiewicza 30, Krakow 30-059, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanna","family":"Tiainen","sequence":"additional","affiliation":[{"name":"Department of Biomaterials, Institute for Clinical Dentistry, University of Oslo, Geitmyrsveien 71, Blindern, P.O. Box 1109, Oslo NO-0317, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e5vard J","family":"Haugen","sequence":"additional","affiliation":[{"name":"Department of Biomaterials, Institute for Clinical Dentistry, University of Oslo, Geitmyrsveien 71, Blindern, P.O. Box 1109, Oslo NO-0317, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dieter","family":"Scharnweber","sequence":"additional","affiliation":[{"name":"Technische Universit\u00e4t Dresden, Institute of Materials Science, Max Bergmann Center of Biomaterials, Budapester Str. 27, Dresden 01-069, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"El\u017cbieta","family":"Pamu\u0142a","sequence":"additional","affiliation":[{"name":"Faculty of Materials Science and Ceramics, Department of Biomaterials and Composites, AGH University of Science and Technology, Al. A. 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