{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T14:38:45Z","timestamp":1781707125602,"version":"3.54.5"},"reference-count":92,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2024,8,20]],"date-time":"2024-08-20T00:00:00Z","timestamp":1724112000000},"content-version":"vor","delay-in-days":232,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["52250710155"],"award-info":[{"award-number":["52250710155"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFA1101200"],"award-info":[{"award-number":["2021YFA1101200"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFA1105501"],"award-info":[{"award-number":["2022YFA1105501"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Retinal degeneration diseases, such as age-related macular degeneration (AMD) and retinitis pigmentosa (RP), initially manifest as dysfunction or death of the retinal pigment epithelium (RPE). Subretinal transplantation of human pluripotent stem cell (hPSC)-derived RPE cells has emerged as a potential therapy for retinal degeneration. However, RPE cells differentiated from hPSCs using current protocols are xeno-containing and are rarely applied in clinical trials. The development of hPSC-derived RPE cell differentiation protocols using xeno-free biomaterials is urgently needed for clinical applications. In this study, two protocols (the activin A and NIC84 protocols) were selected for modification and use in the differentiation of hiPSCs into RPE cells; the chetomin concentration was gradually increased to achieve high differentiation efficiency of RPE cells. The xeno-free extracellular matrix (ECM) proteins, laminin-511, laminin-521 and recombinant vitronectin, were selected as plate-coating substrates, and a Matrigel (xeno-containing ECM)-coated surface was used as a positive control. Healthy, mature hPSC-derived RPE cells were transplanted into 21-day-old Royal College of Surgeons (RCS) rats, a model of retinal degeneration disease. The visual function of RCS rats was evaluated by optomotor response (qOMR) and electroretinography after transplantation of hPSC-derived RPE cells. Our study demonstrated that hPSCs can be efficiently differentiated into RPE cells on LN521-coated dishes using the NIC84 protocol, and that subretinal transplantation of the cell suspensions can delay the progression of vision loss in RCS rats.<\/jats:p>","DOI":"10.1093\/rb\/rbae091","type":"journal-article","created":{"date-parts":[[2024,8,14]],"date-time":"2024-08-14T02:28:09Z","timestamp":1723602489000},"source":"Crossref","is-referenced-by-count":6,"title":["Effects of ECM protein-coated surfaces on the generation of retinal pigment epithelium cells differentiated from human pluripotent stem cells"],"prefix":"10.1093","volume":"11","author":[{"given":"Zeyu","family":"Tian","sequence":"first","affiliation":[{"name":"State Key Laboratory of Ophthalmology, Optometry and Visual Science, Eye Hospital, Wenzhou Medical University , Wenzhou, Zhejiang 325027,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ophthalmology, Optometry and Visual Science, Eye Hospital, Wenzhou Medical University , Wenzhou, Zhejiang 325027,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui-Yu","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Chemical and Materials Engineering, National Central University , Taoyuan 32001, ,","place":["Taiwan China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Ru","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Chemical and Materials Engineering, National Central University , Taoyuan 32001, ,","place":["Taiwan China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Ling","sequence":"additional","affiliation":[{"name":"State Key Laboratory of 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325027,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Gao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ophthalmology, Optometry and Visual Science, Eye Hospital, Wenzhou Medical University , Wenzhou, Zhejiang 325027,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sitian","family":"Cheng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ophthalmology, Optometry and Visual Science, Eye Hospital, Wenzhou Medical University , Wenzhou, Zhejiang 325027,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Remya Rajan","family":"Renuka","sequence":"additional","affiliation":[{"name":"Center for Global Health Research, Saveetha Medical College and Hospitals, Saveetha Institute of Medical and Technical Sciences , Chennai, Tamil Nadu 602105,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suresh Kumar","family":"Subbiah","sequence":"additional","affiliation":[{"name":"Center for Global Health Research, Saveetha Medical College and Hospitals, Saveetha Institute of Medical and Technical Sciences , Chennai, Tamil Nadu 602105,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoping","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Human Genetics, David Geffen School of Medicine, UCLA , Los Angeles, CA 90095,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gwo-Jang","family":"Wu","sequence":"additional","affiliation":[{"name":"Graduate Institute of Medical Sciences and Department of Obstetrics & Gynecology, Tri-Service General Hospital, National Defense Medical Center , Taipei 11490, ,","place":["Taiwan 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