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The peptide-grafted hydrogels promoted the adhesion, proliferation and colony formation of hiPSCs and maintained their pluripotency up to passage 5 under xeno-free conditions. We successfully generated RPE cells from hiPSCs using one of the most suitable xeno-free peptide-grafted hydrogels, KVN2CK (KGCGGKGG-PQVTRGDVFTMP), which was derived from vitronectin, and confirmed the effect of these hiPSC-derived RPE cells in a rat retinal degeneration model (Royal College of Surgeons (RCS) rats) via subretinal transplantation, when we investigated functional improvements in vision in RCS rats after the transplantation of hiPSC-derived RPE cells. Our novel peptide-grafted hydrogels provided a safe and robust platform for generating single-layer hiPSC-derived RPE cells under xeno-free conditions, which indicates the potential of these hydrogels for stem cell therapy for retinal degenerative diseases in the future.<\/jats:p>","DOI":"10.1093\/rb\/rbaf035","type":"journal-article","created":{"date-parts":[[2025,4,26]],"date-time":"2025-04-26T17:11:59Z","timestamp":1745687519000},"source":"Crossref","is-referenced-by-count":4,"title":["Differentiation of human induced pluripotent stem cells into retinal pigment epithelium cells during culture on peptide-grafted hydrogels"],"prefix":"10.1093","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9025-8341","authenticated-orcid":false,"given":"Jun","family":"Liu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Eye Health, Eye Hospital, Wenzhou Medical University , Wenzhou, Zhejiang 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