{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T01:43:01Z","timestamp":1784770981053,"version":"3.55.0"},"reference-count":180,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T00:00:00Z","timestamp":1772755200000},"content-version":"vor","delay-in-days":64,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82271054"],"award-info":[{"award-number":["82271054"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82401321"],"award-info":[{"award-number":["82401321"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,1,22]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Hydrogel-mediated\u2009 gene\u2009 therapy\u2009 has\u2009 achieved significant progress in the field of ophthalmology in recent years. As three-dimensional polymeric networks with high water content, hydrogels exhibit excellent biocompatibility, localized targeting capability and sustained-release properties. These characteristics make them ideal carriers for nucleic acid-based drugs, including DNA, RNA and gene editing systems. They can significantly improve the therapeutic efficacy and delivery kinetics of ocular drug administration, demonstrating unique advantages in the special ocular environment. This review systematically examines the current state of hydrogel-based gene therapy research in ophthalmology. First, recent advances in gene therapy are summarized, and the involved gene types and technical strategies are categorized. Second, various categories of hydrogels are outlined, including photo-responsive, temperature-sensitive and pH-responsive hydrogels. Their key characteristics for ocular gene delivery are also summarized. Furthermore, this article focuses on analyzing the design strategies for hydrogel-mediated gene therapy. Key design considerations include gene encapsulation, stability control and release kinetics. In addition, various gene-hydrogel integration strategies are discussed, including covalent conjugation and physical loading methods. Their impacts on transfection efficiency and therapeutic outcomes are also analyzed. Additionally, cutting-edge applications of hydrogel-based gene therapy in treating ocular diseases are systematically introduced, covering both anterior segment and fundus diseases. Finally, the limitations and future development prospects of these gene therapy hydrogel systems in ophthalmic research are discussed.<\/jats:p>","DOI":"10.1093\/rb\/rbag041","type":"journal-article","created":{"date-parts":[[2026,3,2]],"date-time":"2026-03-02T12:21:56Z","timestamp":1772454116000},"source":"Crossref","is-referenced-by-count":1,"title":["Advances in hydrogel-mediated gene therapy in ophthalmology: future directions and therapeutic potential"],"prefix":"10.1093","volume":"13","author":[{"given":"Qinghe","family":"Zhang","sequence":"first","affiliation":[{"name":"Xiamen University Affiliated Xiamen Eye Center; Fujian Provincial Key Laboratory of Ophthalmology and Visual Science; Fujian Engineering and Research Center of Eye Regenerative Medicine, Eye Institute of Xiamen University; School of Medicine, Xiamen University , Xiamen, Fujian 361005,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, The First Affiliated Hospital of University of South China, Hengyang Medical School, University of South China , Hengyang, Hunan 421001,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yufei","family":"Lv","sequence":"additional","affiliation":[{"name":"Department of Human Anatomy, Hengyang Medical School, University of South China , Hengyang, Hunan 421001,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Tao","sequence":"additional","affiliation":[{"name":"Xiamen University Affiliated Xiamen Eye Center; Fujian Provincial Key Laboratory of Ophthalmology and Visual Science; Fujian Engineering and Research Center of Eye Regenerative Medicine, Eye Institute of Xiamen University; School of Medicine, Xiamen University , Xiamen, Fujian 361005,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinan","family":"Wu","sequence":"additional","affiliation":[{"name":"Xiamen University Affiliated Xiamen Eye Center; Fujian Provincial Key Laboratory of Ophthalmology and Visual Science; Fujian Engineering and Research Center of Eye Regenerative Medicine, Eye Institute of Xiamen University; School of Medicine, Xiamen University , Xiamen, Fujian 361005,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zixuan","family":"Yang","sequence":"additional","affiliation":[{"name":"Xiamen University Affiliated Xiamen Eye Center; Fujian Provincial Key Laboratory of Ophthalmology and Visual Science; Fujian Engineering and Research Center of Eye Regenerative Medicine, Eye Institute of Xiamen University; School of Medicine, Xiamen University , Xiamen, Fujian 361005,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linyan","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, The First Affiliated Hospital of University of South China, Hengyang Medical School, University of South China , Hengyang, Hunan 421001,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caihong","family":"Huang","sequence":"additional","affiliation":[{"name":"Xiamen University Affiliated Xiamen Eye Center; Fujian Provincial Key Laboratory of Ophthalmology and Visual Science; Fujian Engineering and Research Center of Eye Regenerative Medicine, Eye Institute of Xiamen University; School of Medicine, Xiamen University , Xiamen, Fujian 361005,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiuping","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, The First Affiliated Hospital of University of South China, Hengyang Medical School, University of South China , Hengyang, Hunan 421001,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5226-3476","authenticated-orcid":false,"given":"Yi","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, The First Affiliated Hospital of University of South China, Hengyang Medical School, University of South China , Hengyang, Hunan 421001,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zuguo","family":"Liu","sequence":"additional","affiliation":[{"name":"Xiamen University Affiliated Xiamen Eye Center; Fujian Provincial Key Laboratory of Ophthalmology and Visual Science; Fujian Engineering and Research Center of Eye Regenerative Medicine, Eye Institute of Xiamen University; School of Medicine, Xiamen University , Xiamen, Fujian 361005,","place":["China"]},{"name":"Department of Ophthalmology, The First Affiliated Hospital of University of South China, Hengyang Medical School, University of South China , Hengyang, Hunan 421001,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,3,5]]},"reference":[{"key":"2026051501515734700_rbag041-B1","doi-asserted-by":"crossref","first-page":"101011","DOI":"10.1016\/j.preteyeres.2021.101011","article-title":"Regenerative therapy for the cornea","volume":"87","author":"Kumar","year":"2022","journal-title":"Prog Retin Eye Res"},{"key":"2026051501515734700_rbag041-B2","doi-asserted-by":"crossref","first-page":"915","DOI":"10.7150\/thno.103266","article-title":"Advances in adhesive hydrogels applied for ophthalmology: an overview focused on the treatment","volume":"15","author":"Yan","year":"2025","journal-title":"Theranostics"},{"key":"2026051501515734700_rbag041-B3","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1124\/jpet.119.256933","article-title":"Ocular drug delivery: present innovations and future challenges","volume":"370","author":"Gote","year":"2019","journal-title":"J Pharmacol Exp Ther"},{"key":"2026051501515734700_rbag041-B4","doi-asserted-by":"crossref","first-page":"11206721231190106","DOI":"10.1177\/11206721231190106","article-title":"Ocular surface in posterior segment surgery","volume":"27","author":"Coassin","year":"2023","journal-title":"Eur J Ophthalmol"},{"key":"2026051501515734700_rbag041-B5","doi-asserted-by":"crossref","first-page":"112212","DOI":"10.1016\/j.msec.2021.112212","article-title":"Hydrogels-based ophthalmic drug delivery systems for treatment of ocular diseases","volume":"127","author":"Fang","year":"2021","journal-title":"Mater Sci Eng C Mater Biol Appl"},{"key":"2026051501515734700_rbag041-B6","doi-asserted-by":"crossref","first-page":"e231067","DOI":"10.1001\/jamaophthalmol.2023.1067","article-title":"Choroidal detachment after intravitreal injection of faricimab","volume":"141","author":"Schonbach","year":"2023","journal-title":"JAMA Ophthalmol"},{"key":"2026051501515734700_rbag041-B7","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1097\/APO.0000000000000146","article-title":"Intravitreal injection\u2014technique and safety","volume":"4","author":"Lai","year":"2015","journal-title":"Asia Pac J Ophthalmol (Phila)"},{"key":"2026051501515734700_rbag041-B8","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.cbpa.2020.01.012","article-title":"Biocatalysis in drug discovery and development","volume":"55","author":"Fryszkowska","year":"2020","journal-title":"Curr Opin Chem Biol"},{"key":"2026051501515734700_rbag041-B9","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.cbi.2008.11.013","article-title":"Overview: evaluation of metabolism-based drug toxicity in drug development","volume":"179","author":"Li","year":"2009","journal-title":"Chem Biol Interact"},{"key":"2026051501515734700_rbag041-B10","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1016\/S0140-6736(23)01952-9","article-title":"A new age of precision gene therapy","volume":"403","author":"Schambach","year":"2024","journal-title":"Lancet"},{"key":"2026051501515734700_rbag041-B11","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1186\/s12951-024-02462-z","article-title":"Developing hydrogels for gene therapy and tissue engineering","volume":"22","author":"Su","year":"2024","journal-title":"J Nanobiotechnology"},{"key":"2026051501515734700_rbag041-B12","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1056\/NEJMra1706910","article-title":"Gene therapy","volume":"381","author":"High","year":"2019","journal-title":"N Engl J Med"},{"key":"2026051501515734700_rbag041-B13","doi-asserted-by":"crossref","first-page":"101082","DOI":"10.1016\/j.mtbio.2024.101082","article-title":"Research progress of photo-crosslink hydrogels in ophthalmology: a comprehensive review focus on the applications","volume":"26","author":"Zhang","year":"2024","journal-title":"Mater Today Bio"},{"key":"2026051501515734700_rbag041-B14","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1038\/s41392-024-01852-x","article-title":"Harnessing the potential of hydrogels for advanced therapeutic applications: current achievements and future directions","volume":"9","author":"Lu","year":"2024","journal-title":"Signal Transduct Target Ther"},{"key":"2026051501515734700_rbag041-B15","doi-asserted-by":"crossref","first-page":"39784","DOI":"10.1021\/acsami.4c05425","article-title":"Transforming cell-drug interaction through granular hydrogel-mediated delivery of polyplex nanoparticles for enhanced safety and extended efficacy in gene therapy","volume":"16","author":"Zhang","year":"2024","journal-title":"ACS Appl Mater Interfaces"},{"key":"2026051501515734700_rbag041-B16","doi-asserted-by":"crossref","first-page":"6421","DOI":"10.1016\/j.cell.2024.10.029","article-title":"The cellular dogma","volume":"187","author":"Quake","year":"2024","journal-title":"Cell"},{"key":"2026051501515734700_rbag041-B17","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1007\/978-1-0716-3346-5_6","article-title":"The interactome of protein, DNA, and RNA","volume":"2695","author":"Yan","year":"2023","journal-title":"Methods Mol Biol"},{"key":"2026051501515734700_rbag041-B18","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1038\/sj.gt.3302251","article-title":"Gene therapy progress and prospects: transcription regulatory systems","volume":"11","author":"Toniatti","year":"2004","journal-title":"Gene Ther"},{"key":"2026051501515734700_rbag041-B19","doi-asserted-by":"crossref","first-page":"3828","DOI":"10.1039\/D1CS00617G","article-title":"Emerging mRNA technologies: delivery strategies and biomedical applications","volume":"51","author":"Xiao","year":"2022","journal-title":"Chem Soc Rev"},{"key":"2026051501515734700_rbag041-B20","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1038\/s41580-022-00573-9","article-title":"Regulation of ribosomal RNA gene copy number, transcription and nucleolus organization in eukaryotes","volume":"24","author":"Hori","year":"2023","journal-title":"Nat Rev Mol Cell Biol"},{"key":"2026051501515734700_rbag041-B21","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1007\/s40259-022-00549-3","article-title":"Therapeutic siRNA: state-of-the-art and future perspectives","volume":"36","author":"Friedrich","year":"2022","journal-title":"BioDrugs"},{"key":"2026051501515734700_rbag041-B22","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1016\/j.tig.2022.02.006","article-title":"Emerging concepts of miRNA therapeutics: from cells to clinic","volume":"38","author":"Diener","year":"2022","journal-title":"Trends Genet"},{"key":"2026051501515734700_rbag041-B23","first-page":"11206721231190106","article-title":"Nonsense-mediated mRNA decay begins where translation ends","volume":"27","author":"Karousis","year":"2019","journal-title":"Cold Spring Harb Perspect Biol"},{"key":"2026051501515734700_rbag041-B24","first-page":"30"},{"key":"2026051501515734700_rbag041-B25","doi-asserted-by":"crossref","first-page":"174178","DOI":"10.1016\/j.ejphar.2021.174178","article-title":"siRNA: mechanism of action, challenges, and therapeutic approaches","volume":"905","author":"Alshaer","year":"2021","journal-title":"Eur J Pharmacol"},{"key":"2026051501515734700_rbag041-B26","doi-asserted-by":"crossref","first-page":"108329","DOI":"10.1016\/j.exer.2020.108329","article-title":"Role of small interfering RNA (siRNA) in targeting ocular neovascularization: a review","volume":"202","author":"Supe","year":"2021","journal-title":"Exp Eye Res"},{"key":"2026051501515734700_rbag041-B27","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1007\/978-1-0716-1298-9_23","article-title":"siRNA therapeutics in ocular diseases","volume":"2282","author":"Moreno-Montanes","year":"2021","journal-title":"Methods Mol Biol"},{"key":"2026051501515734700_rbag041-B28","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.molmed.2023.10.005","article-title":"Three 'E' challenges for siRNA drug development","volume":"30","author":"Guo","year":"2024","journal-title":"Trends Mol Med"},{"key":"2026051501515734700_rbag041-B29","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1016\/j.addr.2009.04.004","article-title":"siRNA vs. shRNA: similarities and differences","volume":"61","author":"Rao","year":"2009","journal-title":"Adv Drug Deliv Rev"},{"key":"2026051501515734700_rbag041-B30","first-page":"141","article-title":"Short hairpin RNA (shRNA): design, delivery, and assessment of gene knockdown","volume":"629","author":"Moore","year":"2010","journal-title":"Methods Mol Biol"},{"key":"2026051501515734700_rbag041-B31","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.biomaterials.2017.06.001","article-title":"Controlled and sustained delivery of siRNA\/NPs from hydrogels expedites bone fracture healing","volume":"139","author":"Wang","year":"2017","journal-title":"Biomaterials"},{"key":"2026051501515734700_rbag041-B32","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1038\/nrg2843","article-title":"The widespread regulation of microRNA biogenesis, function and decay","volume":"11","author":"Krol","year":"2010","journal-title":"Nat Rev Genet"},{"key":"2026051501515734700_rbag041-B33","doi-asserted-by":"crossref","first-page":"586","DOI":"10.1038\/nsmb.2296","article-title":"The mechanics of miRNA-mediated gene silencing: a look under the hood of miRISC","volume":"19","author":"Fabian","year":"2012","journal-title":"Nat Struct Mol Biol"},{"key":"2026051501515734700_rbag041-B34","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1146\/annurev-biochem-060308-103103","article-title":"Regulation of mRNA translation and stability by microRNAs","volume":"79","author":"Fabian","year":"2010","journal-title":"Annu Rev Biochem"},{"key":"2026051501515734700_rbag041-B35","doi-asserted-by":"crossref","first-page":"6249","DOI":"10.3390\/ijms20246249","article-title":"Deciphering miRNAs\u2019 action through miRNA editing","volume":"20","author":"Correia de Sousa","year":"2019","journal-title":"Int J Mol Sci"},{"key":"2026051501515734700_rbag041-B36","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1186\/s13073-017-0450-0","article-title":"Advances in the delivery of RNA therapeutics: from concept to clinical reality","volume":"9","author":"Kaczmarek","year":"2017","journal-title":"Genome Med"},{"key":"2026051501515734700_rbag041-B37","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1038\/ng1092-93","article-title":"A brief history of gene therapy","volume":"2","author":"Friedmann","year":"1992","journal-title":"Nat Genet"},{"key":"2026051501515734700_rbag041-B38","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1038\/s41434-020-00197-8","article-title":"Gene therapy and gene correction: targets, progress, and challenges for treating human diseases","volume":"29","author":"Cring","year":"2022","journal-title":"Gene Ther"},{"key":"2026051501515734700_rbag041-B39","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1038\/s41392-024-01780-w","article-title":"Adeno-associated virus as a delivery vector for gene therapy of human diseases","volume":"9","author":"Wang","year":"2024","journal-title":"Signal Transduct Target Ther"},{"key":"2026051501515734700_rbag041-B40","doi-asserted-by":"crossref","first-page":"1642","DOI":"10.1002\/emmm.201202287","article-title":"Gene therapy on the move","volume":"5","author":"Kaufmann","year":"2013","journal-title":"EMBO Mol Med"},{"key":"2026051501515734700_rbag041-B41","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1038\/mt.2016.10","article-title":"Genome-editing technologies for gene and cell therapy","volume":"24","author":"Maeder","year":"2016","journal-title":"Mol Ther"},{"key":"2026051501515734700_rbag041-B42","doi-asserted-by":"crossref","first-page":"251","DOI":"10.4314\/njm.v17i3.37390","article-title":"Bone marrow transplantation (BMT) and gene replacement therapy (GRT) in sickle cell anemia","volume":"17","author":"Misaki","year":"2008","journal-title":"Niger J Med"},{"key":"2026051501515734700_rbag041-B43","doi-asserted-by":"crossref","first-page":"1130","DOI":"10.3390\/ijms24021130","article-title":"Gene therapy in ALS and SMA: advances, challenges and perspectives","volume":"24","author":"Lejman","year":"2023","journal-title":"Int J Mol Sci"},{"key":"2026051501515734700_rbag041-B44","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1016\/j.biotechadv.2012.10.001","article-title":"Development of hybrid viral vectors for gene therapy","volume":"31","author":"Huang","year":"2013","journal-title":"Biotechnol Adv"},{"key":"2026051501515734700_rbag041-B45","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1038\/s41392-021-00487-6","article-title":"Viral vector platforms within the gene therapy landscape","volume":"6","author":"Bulcha","year":"2021","journal-title":"Signal Transduct Target Ther"},{"key":"2026051501515734700_rbag041-B46","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1007\/s40259-017-0234-5","article-title":"Adeno-associated virus (AAV) as a vector for gene therapy","volume":"31","author":"Naso","year":"2017","journal-title":"BioDrugs"},{"key":"2026051501515734700_rbag041-B47","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1089\/hum.2023.018","article-title":"Complement activation by adeno-associated virus-neutralizing antibody complexes","volume":"34","author":"West","year":"2023","journal-title":"Hum Gene Ther"},{"key":"2026051501515734700_rbag041-B48","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1016\/j.ymthe.2019.12.010","article-title":"AAV vector immunogenicity in humans: a long journey to successful gene transfer","volume":"28","author":"Costa Verdera","year":"2020","journal-title":"Mol Ther"},{"key":"2026051501515734700_rbag041-B49","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1167\/iovs.64.7.39","article-title":"Gene therapy for retinal degenerative diseases: progress, challenges, and future directions","volume":"64","author":"Drag","year":"2023","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"2026051501515734700_rbag041-B50","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1038\/mt.2010.293","article-title":"Suppression and replacement gene therapy for autosomal dominant disease in a murine model of dominant retinitis pigmentosa","volume":"19","author":"Millington-Ward","year":"2011","journal-title":"Mol Ther"},{"key":"2026051501515734700_rbag041-B51","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/S0074-7696(02)19011-5","article-title":"Gene silencing","volume":"219","author":"Wassenegger","year":"2002","journal-title":"Int Rev Cytol"},{"key":"2026051501515734700_rbag041-B52","doi-asserted-by":"crossref","first-page":"2068","DOI":"10.1038\/s41587-025-02576-1","article-title":"Multiplexed inhibition of immunosuppressive genes with Cas13d for combinatorial cancer immunotherapy","volume":"43","author":"Zhang","year":"2025","journal-title":"Nat Biotechnol"},{"key":"2026051501515734700_rbag041-B53","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1016\/j.tig.2018.05.004","article-title":"Gene editing on center stage","volume":"34","author":"Bak","year":"2018","journal-title":"Trends Genet"},{"key":"2026051501515734700_rbag041-B54","first-page":"582","article-title":"Gene editing technology and its recent progress in disease therapy","volume":"41","author":"Niu","year":"2019","journal-title":"Yi Chuan"},{"key":"2026051501515734700_rbag041-B55","doi-asserted-by":"crossref","first-page":"770","DOI":"10.1038\/s41576-018-0059-1","article-title":"Base editing: precision chemistry on the genome and transcriptome of living cells","volume":"19","author":"Rees","year":"2018","journal-title":"Nat Rev Genet"},{"key":"2026051501515734700_rbag041-B56","doi-asserted-by":"crossref","first-page":"115026","DOI":"10.1016\/j.addr.2023.115026","article-title":"Gene editing therapeutics based on mRNA delivery","volume":"200","author":"Popovitz","year":"2023","journal-title":"Adv Drug Deliv Rev"},{"key":"2026051501515734700_rbag041-B57","doi-asserted-by":"crossref","first-page":"eadd8643","DOI":"10.1126\/science.add8643","article-title":"CRISPR technology: a decade of genome editing is only the beginning","volume":"379","author":"Wang","year":"2023","journal-title":"Science"},{"key":"2026051501515734700_rbag041-B58","doi-asserted-by":"crossref","first-page":"1102","DOI":"10.1016\/j.tplants.2019.09.006","article-title":"CRISPR\/cas system: recent advances and future prospects for genome editing","volume":"24","author":"Manghwar","year":"2019","journal-title":"Trends Plant Sci"},{"key":"2026051501515734700_rbag041-B59","doi-asserted-by":"crossref","first-page":"e2300195","DOI":"10.1002\/advs.202300195","article-title":"Progress and perspective of CRISPR-Cas9 technology in translational medicine","volume":"10","author":"Zheng","year":"2023","journal-title":"Adv Sci (Weinh)"},{"key":"2026051501515734700_rbag041-B60","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1186\/s40694-024-00180-7","article-title":"NHEJ and HDR can occur simultaneously during gene integration into the genome of Aspergillus niger","volume":"11","author":"Fritsche","year":"2024","journal-title":"Fungal Biol Biotechnol"},{"key":"2026051501515734700_rbag041-B61","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1038\/nrm.2017.48","article-title":"Non-homologous DNA end joining and alternative pathways to double-strand break repair","volume":"18","author":"Chang","year":"2017","journal-title":"Nat Rev Mol Cell Biol"},{"key":"2026051501515734700_rbag041-B62","doi-asserted-by":"crossref","first-page":"698","DOI":"10.1038\/s41580-019-0152-0","article-title":"DNA double-strand break repair-pathway choice in somatic mammalian cells","volume":"20","author":"Scully","year":"2019","journal-title":"Nat Rev Mol Cell Biol"},{"key":"2026051501515734700_rbag041-B63","doi-asserted-by":"crossref","first-page":"e2401710","DOI":"10.1002\/advs.202401710","article-title":"Genome editing VEGFA prevents corneal neovascularization in vivo","volume":"11","author":"Zeng","year":"2024","journal-title":"Adv Sci (Weinh)"},{"key":"2026051501515734700_rbag041-B64","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1038\/nbt.3437","article-title":"Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9","volume":"34","author":"Doench","year":"2016","journal-title":"Nat Biotechnol"},{"key":"2026051501515734700_rbag041-B65","doi-asserted-by":"crossref","first-page":"4067","DOI":"10.1016\/j.cell.2022.09.026","article-title":"Structural basis for Cas9 off-target activity","volume":"185","author":"Pacesa","year":"2022","journal-title":"Cell"},{"key":"2026051501515734700_rbag041-B66","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1038\/nature17946","article-title":"Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage","volume":"533","author":"Komor","year":"2016","journal-title":"Nature"},{"key":"2026051501515734700_rbag041-B67","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1038\/nbt.3803","article-title":"Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions","volume":"35","author":"Kim","year":"2017","journal-title":"Nat Biotechnol"},{"key":"2026051501515734700_rbag041-B68","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1038\/s41586-019-1711-4","article-title":"Search-and-replace genome editing without double-strand breaks or donor DNA","volume":"576","author":"Anzalone","year":"2019","journal-title":"Nature"},{"key":"2026051501515734700_rbag041-B69","doi-asserted-by":"crossref","first-page":"2394","DOI":"10.1038\/s41467-025-57628-6","article-title":"In vivo prime editing rescues photoreceptor degeneration in nonsense mutant retinitis pigmentosa","volume":"16","author":"Fu","year":"2025","journal-title":"Nat Commun"},{"key":"2026051501515734700_rbag041-B70","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1038\/nature24644","article-title":"Programmable base editing of AT to GC in genomic DNA without DNA cleavage","volume":"551","author":"Gaudelli","year":"2017","journal-title":"Nature"},{"key":"2026051501515734700_rbag041-B71","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.carbpol.2017.11.013","article-title":"Efficient gene delivery by oligochitosan conjugated serum albumin: facile synthesis, polyplex stability, and transfection","volume":"183","author":"Kumari","year":"2018","journal-title":"Carbohydr Polym"},{"key":"2026051501515734700_rbag041-B72","doi-asserted-by":"crossref","first-page":"7736","DOI":"10.3390\/ijms24097736","article-title":"Viral vector-based gene therapy","volume":"24","author":"Li","year":"2023","journal-title":"IJMS"},{"key":"2026051501515734700_rbag041-B73","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1208\/s12248-021-00608-7","article-title":"Non-viral vectors in gene therapy: recent development, challenges, and prospects","volume":"23","author":"Zu","year":"2021","journal-title":"AAPS J"},{"key":"2026051501515734700_rbag041-B74","doi-asserted-by":"crossref","first-page":"105834","DOI":"10.1016\/j.ebiom.2025.105834","article-title":"Viral and non-viral vectors in gene therapy: current state and clinical perspectives","volume":"118","author":"Geng","year":"2025","journal-title":"EBioMedicine"},{"key":"2026051501515734700_rbag041-B75","first-page":"11421"},{"key":"2026051501515734700_rbag041-B76","doi-asserted-by":"crossref","first-page":"114612","DOI":"10.1016\/j.bios.2022.114612","article-title":"Hexa-BODIPY-cyclotriphosphazene based nanoparticle for NIR fluorescence\/photoacoustic dual-modal imaging and photothermal cancer therapy","volume":"216","author":"Kwon","year":"2022","journal-title":"Biosens Bioelectron"},{"key":"2026051501515734700_rbag041-B77","doi-asserted-by":"crossref","first-page":"e2307078","DOI":"10.1002\/smll.202307078","article-title":"Gene and photothermal combination therapy: principle, materials, and amplified anticancer intervention","volume":"20","author":"Tang","year":"2024","journal-title":"Small"},{"key":"2026051501515734700_rbag041-B78","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1007\/978-981-15-3258-0_6","article-title":"Visible light-curable hydrogel systems for tissue engineering and drug delivery","volume":"1249","author":"Yang","year":"2020","journal-title":"Adv Exp Med Biol"},{"key":"2026051501515734700_rbag041-B79","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1038\/nature11409","article-title":"Highly stretchable and tough hydrogels","volume":"489","author":"Sun","year":"2012","journal-title":"Nature"},{"key":"2026051501515734700_rbag041-B80","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.addr.2009.07.019","article-title":"Chitosan-based hydrogels for controlled, localized drug delivery","volume":"62","author":"Bhattarai","year":"2010","journal-title":"Adv Drug Deliv Rev"},{"key":"2026051501515734700_rbag041-B81","doi-asserted-by":"crossref","first-page":"107100","DOI":"10.1016\/j.phrs.2024.107100","article-title":"Novel nano-drug delivery system for natural products and their application","volume":"201","author":"Huang","year":"2024","journal-title":"Pharmacol Res"},{"key":"2026051501515734700_rbag041-B82","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/j.jconrel.2018.04.024","article-title":"Tissue engineering: still facing a long way ahead","volume":"279","author":"Hassanzadeh","year":"2018","journal-title":"J Control Release"},{"key":"2026051501515734700_rbag041-B83","doi-asserted-by":"crossref","first-page":"16071","DOI":"10.1038\/natrevmats.2016.71","article-title":"Designing hydrogels for controlled drug delivery","volume":"1","author":"Li","year":"2016","journal-title":"Nat Rev Mater"},{"key":"2026051501515734700_rbag041-B84","doi-asserted-by":"crossref","first-page":"e1520","DOI":"10.1002\/wnan.1520","article-title":"Double network hydrogel for tissue engineering","volume":"10","author":"Gu","year":"2018","journal-title":"Wiley Interdiscip Rev Nanomed Nanobiotechnol"},{"key":"2026051501515734700_rbag041-B85","doi-asserted-by":"crossref","first-page":"102301","DOI":"10.1016\/j.cis.2020.102301","article-title":"Stimulus-responsive luminescent hydrogels: design and applications","volume":"286","author":"Cheng","year":"2020","journal-title":"Adv Colloid Interface Sci"},{"key":"2026051501515734700_rbag041-B86","doi-asserted-by":"crossref","first-page":"119726","DOI":"10.1016\/j.carbpol.2022.119726","article-title":"Recent developments in chitosan hydrogels carrying natural bioactive compounds","volume":"294","author":"Do","year":"2022","journal-title":"Carbohydr Polym"},{"key":"2026051501515734700_rbag041-B87","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.jconrel.2022.07.038","article-title":"Customizing nano-chitosan for sustainable drug delivery","volume":"350","author":"Saeedi","year":"2022","journal-title":"J Control Release"},{"key":"2026051501515734700_rbag041-B88","doi-asserted-by":"crossref","first-page":"12687","DOI":"10.1021\/acsnano.1c04206","article-title":"Functional hydrogels as wound dressing to enhance wound healing","volume":"15","author":"Liang","year":"2021","journal-title":"ACS Nano"},{"key":"2026051501515734700_rbag041-B89","doi-asserted-by":"crossref","first-page":"1813","DOI":"10.1007\/s11427-019-1710-8","article-title":"Photosensitive hydrogels: from structure, mechanisms, design to bioapplications","volume":"63","author":"Ji","year":"2020","journal-title":"Sci China Life Sci"},{"key":"2026051501515734700_rbag041-B90","doi-asserted-by":"crossref","first-page":"121327","DOI":"10.1016\/j.carbpol.2023.121327","article-title":"Temperature-sensitive multifunctional intelligent responsive hydrogel based on carboxymethyl agarose and N-isopropylacrylamide: controlled drug release and accelerated wound healing","volume":"322","author":"Cai","year":"2023","journal-title":"Carbohydr Polym"},{"key":"2026051501515734700_rbag041-B91","doi-asserted-by":"crossref","first-page":"1923","DOI":"10.1021\/jo802427k","article-title":"Pd-catalyzed C-C cross-coupling reactions within a thermoresponsive and pH-responsive and chelating polymeric hydrogel","volume":"74","author":"Wang","year":"2009","journal-title":"J Org Chem"},{"key":"2026051501515734700_rbag041-B92","doi-asserted-by":"crossref","first-page":"e1801234","DOI":"10.1002\/adhm.201801234","article-title":"A photoresponsive hyaluronan hydrogel nanocomposite for dynamic macrophage immunomodulation","volume":"8","author":"Wang","year":"2019","journal-title":"Adv Healthc Mater"},{"key":"2026051501515734700_rbag041-B93","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1021\/acs.accounts.4c00548","article-title":"Photoresponsive chemistries for user-directed hydrogel network modulation to investigate cell-matrix interactions","volume":"58","author":"Ohnsorg","year":"2025","journal-title":"Acc Chem Res"},{"key":"2026051501515734700_rbag041-B94","doi-asserted-by":"crossref","first-page":"4218","DOI":"10.1021\/acs.biomac.3c00536","article-title":"Photocuring and gelatin-based antibacterial hydrogel for skin care","volume":"24","author":"Wang","year":"2023","journal-title":"Biomacromolecules"},{"key":"2026051501515734700_rbag041-B95","doi-asserted-by":"crossref","first-page":"e2300192","DOI":"10.1002\/adhm.202300192","article-title":"ECM-Like adhesive hydrogel for the regeneration of large corneal stromal defects","volume":"12","author":"Wang","year":"2023","journal-title":"Adv Healthc Mater"},{"key":"2026051501515734700_rbag041-B96","doi-asserted-by":"crossref","first-page":"122754","DOI":"10.1016\/j.biomaterials.2024.122754","article-title":"Corneal stromal structure replicating humanized hydrogel patch for sutureless repair of deep anterior-corneal defect","volume":"313","author":"Zhao","year":"2025","journal-title":"Biomaterials"},{"key":"2026051501515734700_rbag041-B97","doi-asserted-by":"crossref","first-page":"e2302889","DOI":"10.1002\/adhm.202302889","article-title":"Biomimetic corneal stroma for scarless corneal wound healing via structural restoration and microenvironment modulation","volume":"13","author":"Huang","year":"2024","journal-title":"Adv Healthc Mater"},{"key":"2026051501515734700_rbag041-B98","doi-asserted-by":"crossref","first-page":"e2300996","DOI":"10.1002\/smtd.202300996","article-title":"Photocurable and temperature-sensitive bioadhesive hydrogels for sutureless sealing of full-thickness corneal wounds","volume":"8","author":"Wang","year":"2024","journal-title":"Small Methods"},{"key":"2026051501515734700_rbag041-B99","first-page":"196","article-title":"Natural polymer-derived photocurable bioadhesive hydrogels for sutureless keratoplasty","volume":"8","author":"Zhao","year":"2022","journal-title":"Bioact Mater"},{"key":"2026051501515734700_rbag041-B100","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1021\/mz500230p","article-title":"Coumarin-based photodegradable hydrogel: design, synthesis, gelation, and degradation kinetics","volume":"3","author":"Azagarsamy","year":"2014","journal-title":"ACS Macro Lett"},{"key":"2026051501515734700_rbag041-B101","doi-asserted-by":"crossref","first-page":"4007","DOI":"10.1016\/j.bmcl.2006.05.013","article-title":"Photocleavage of o-nitrobenzyl ether derivatives for rapid biomedical release applications","volume":"16","author":"Kim","year":"2006","journal-title":"Bioorg Med Chem Lett"},{"key":"2026051501515734700_rbag041-B102","doi-asserted-by":"crossref","first-page":"117609","DOI":"10.1016\/j.carbpol.2020.117609","article-title":"Photo-induced programmable degradation of carboxymethyl chitosan-based hydrogels","volume":"256","author":"Wei","year":"2021","journal-title":"Carbohydr Polym"},{"key":"2026051501515734700_rbag041-B103","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.actbio.2021.04.009","article-title":"PEG-based thermosensitive and biodegradable hydrogels","volume":"128","author":"Shi","year":"2021","journal-title":"Acta Biomater"},{"key":"2026051501515734700_rbag041-B104","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.jconrel.2014.04.056","article-title":"In situ gelling pH- and temperature-sensitive biodegradable block copolymer hydrogels for drug delivery","volume":"193","author":"Singh","year":"2014","journal-title":"J Control Release"},{"key":"2026051501515734700_rbag041-B105","first-page":"100911","article-title":"Recent advances on thermosensitive hydrogels-mediated precision therapy","volume":"19","author":"Chen","year":"2024","journal-title":"Asian J Pharm Sci"},{"key":"2026051501515734700_rbag041-B106","doi-asserted-by":"crossref","first-page":"121320","DOI":"10.1016\/j.biomaterials.2021.121320","article-title":"Exosomes-loaded thermosensitive hydrogels for corneal epithelium and stroma regeneration","volume":"280","author":"Tang","year":"2022","journal-title":"Biomaterials"},{"key":"2026051501515734700_rbag041-B107","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.jconrel.2021.11.027","article-title":"A spatiotemporal release platform based on pH\/ROS stimuli-responsive hydrogel in wound repairing","volume":"341","author":"Wu","year":"2022","journal-title":"J Control Release"},{"key":"2026051501515734700_rbag041-B108","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1021\/acspolymersau.1c00049","article-title":"Effect of pH on the properties of hydrogels cross-linked via dynamic thia-michael addition bonds","volume":"2","author":"FitzSimons","year":"2022","journal-title":"ACS Polym Au"},{"key":"2026051501515734700_rbag041-B109","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1038\/s41578-023-00617-2","article-title":"Responsive biomaterials: optimizing control of cancer immunotherapy","volume":"9","author":"Xue","year":"2023","journal-title":"Nat Rev Mater"},{"key":"2026051501515734700_rbag041-B110","doi-asserted-by":"crossref","first-page":"100582","DOI":"10.1016\/j.mtbio.2023.100582","article-title":"Recent progress of antibacterial hydrogels in wound dressings","volume":"19","author":"Jia","year":"2023","journal-title":"Mater Today Bio"},{"key":"2026051501515734700_rbag041-B111","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.biomaterials.2016.01.052","article-title":"One-pot synthesis of pH-responsive hybrid nanogel particles for the intracellular delivery of small interfering RNA","volume":"87","author":"Khaled","year":"2016","journal-title":"Biomaterials"},{"key":"2026051501515734700_rbag041-B112","doi-asserted-by":"crossref","first-page":"6326","DOI":"10.1039\/D1TB00728A","article-title":"Reactive oxygen species (ROS): utilizing injectable antioxidative hydrogels and ROS-producing therapies to manage the double-edged sword","volume":"9","author":"He","year":"2021","journal-title":"J Mater Chem B"},{"key":"2026051501515734700_rbag041-B113","doi-asserted-by":"crossref","first-page":"1600124","DOI":"10.1002\/advs.201600124","article-title":"Reactive-oxygen-species-responsive drug delivery systems: promises and challenges","volume":"4","author":"Saravanakumar","year":"2017","journal-title":"Adv Sci (Weinh)"},{"key":"2026051501515734700_rbag041-B114","doi-asserted-by":"crossref","first-page":"e2409562","DOI":"10.1002\/advs.202409562","article-title":"ROS-responsive microneedle patches enable peri-lacrimal gland therapeutic administration for long-acting therapy of Sjogren\u2019s syndrome-related dry eye","volume":"12","author":"Mu","year":"2025","journal-title":"Adv Sci (Weinh)"},{"key":"2026051501515734700_rbag041-B115","doi-asserted-by":"crossref","first-page":"5694","DOI":"10.1073\/pnas.1601992113","article-title":"Aquaporin gene therapy corrects sjogren\u2019s syndrome phenotype in mice","volume":"113","author":"Lai","year":"2016","journal-title":"Proc Natl Acad Sci USA"},{"key":"2026051501515734700_rbag041-B116","doi-asserted-by":"crossref","first-page":"796","DOI":"10.3390\/polym11050796","article-title":"Characterization and optimization of PLA stereocomplexed hydrogels for local gene delivery systems","volume":"11","author":"Liu","year":"2019","journal-title":"Polymers (Basel)"},{"key":"2026051501515734700_rbag041-B117","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.ijpharm.2011.05.036","article-title":"Non-condensing polymeric nanoparticles for targeted gene and siRNA delivery","volume":"427","author":"Xu","year":"2012","journal-title":"Int J Pharm"},{"key":"2026051501515734700_rbag041-B118","doi-asserted-by":"crossref","first-page":"1223","DOI":"10.1515\/pac-2016-1025","article-title":"Tailoring trehalose for biomedical and biotechnological applications","volume":"89","author":"O\u2019Neill","year":"2017","journal-title":"Pure Appl Chem"},{"key":"2026051501515734700_rbag041-B119","doi-asserted-by":"crossref","first-page":"797094","DOI":"10.3389\/fchem.2021.797094","article-title":"RNA hydrogel combined with MnO2 nanoparticles as a Nano-Vaccine to treat triple negative breast cancer","volume":"9","author":"Wang","year":"2021","journal-title":"Front Chem"},{"key":"2026051501515734700_rbag041-B120","doi-asserted-by":"crossref","first-page":"8050","DOI":"10.1021\/nn5020787","article-title":"Injectable graphene oxide\/hydrogel-based angiogenic gene delivery system for vasculogenesis and cardiac repair","volume":"8","author":"Paul","year":"2014","journal-title":"ACS Nano"},{"key":"2026051501515734700_rbag041-B121","doi-asserted-by":"crossref","first-page":"1407","DOI":"10.1038\/mt.2011.111","article-title":"Engineering biomaterial systems to enhance viral vector gene delivery","volume":"19","author":"Jang","year":"2011","journal-title":"Mol Ther"},{"key":"2026051501515734700_rbag041-B122","doi-asserted-by":"crossref","first-page":"490","DOI":"10.2174\/1574888X12666170612102706","article-title":"The formation mechanism of hydrogels","volume":"13","author":"Lu","year":"2018","journal-title":"Curr Stem Cell Res Ther"},{"key":"2026051501515734700_rbag041-B123","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/S0169-409X(01)00240-X","article-title":"Novel crosslinking methods to design hydrogels","volume":"54","author":"Hennink","year":"2002","journal-title":"Adv Drug Deliv Rev"},{"key":"2026051501515734700_rbag041-B124","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1039\/C8BM01246F","article-title":"Advances in crosslinking strategies of biomedical hydrogels","volume":"7","author":"Hu","year":"2019","journal-title":"Biomater Sci"},{"key":"2026051501515734700_rbag041-B125","doi-asserted-by":"crossref","first-page":"2218","DOI":"10.1038\/s41467-023-37850-w","article-title":"Adaptable covalently cross-linked fibers","volume":"14","author":"Tan","year":"2023","journal-title":"Nat Commun"},{"key":"2026051501515734700_rbag041-B126","doi-asserted-by":"crossref","first-page":"15342","DOI":"10.1002\/anie.201907670","article-title":"Stimuli-responsive biomolecule-based hydrogels and their applications","volume":"59","author":"Vazquez-Gonzalez","year":"2020","journal-title":"Angew Chem Int Ed Engl"},{"key":"2026051501515734700_rbag041-B127","first-page":"313"},{"key":"2026051501515734700_rbag041-B128","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1016\/j.biomaterials.2007.10.010","article-title":"Dual-role self-assembling nanoplexes for efficient gene transfection and sustained gene delivery","volume":"29","author":"Agarwal","year":"2008","journal-title":"Biomaterials"},{"key":"2026051501515734700_rbag041-B129","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1186\/s12951-025-03523-7","article-title":"Transdermal delivery of CRISPR\/Cas9-mediated melanoma gene therapy via polyamines-modified thermosensitive hydrogels","volume":"23","author":"Li","year":"2025","journal-title":"J Nanobiotechnology"},{"key":"2026051501515734700_rbag041-B130","doi-asserted-by":"crossref","first-page":"1423","DOI":"10.1021\/bc050146t","article-title":"Preparation and evaluation of thiol-modified gelatin nanoparticles for intracellular DNA delivery in response to glutathione","volume":"16","author":"Kommareddy","year":"2005","journal-title":"Bioconjug Chem"},{"key":"2026051501515734700_rbag041-B131","doi-asserted-by":"crossref","first-page":"986","DOI":"10.1038\/sj.gt.3301189","article-title":"A combination of poloxamers increases gene expression of plasmid DNA in skeletal muscle","volume":"7","author":"Lemieux","year":"2000","journal-title":"Gene Ther"},{"key":"2026051501515734700_rbag041-B132","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1186\/s12951-023-02044-5","article-title":"Emerging non-viral vectors for gene delivery","volume":"21","author":"Wang","year":"2023","journal-title":"J Nanobiotechnology"},{"key":"2026051501515734700_rbag041-B133","doi-asserted-by":"crossref","first-page":"e2401895","DOI":"10.1002\/adhm.202401895","article-title":"Hydrogels for nucleic acid drugs delivery","volume":"13","author":"Liu","year":"2024","journal-title":"Adv Healthc Mater"},{"key":"2026051501515734700_rbag041-B134","doi-asserted-by":"crossref","first-page":"1285","DOI":"10.1007\/s11427-021-2116-2","article-title":"Recent advances in RNA structurome","volume":"65","author":"Xu","year":"2022","journal-title":"Sci China Life Sci"},{"key":"2026051501515734700_rbag041-B135","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1038\/s41570-019-0147-6","article-title":"The chemistry and applications of RNA 2'-OH acylation","volume":"4","author":"Velema","year":"2020","journal-title":"Nat Rev Chem"},{"key":"2026051501515734700_rbag041-B136","doi-asserted-by":"crossref","first-page":"15968","DOI":"10.1039\/D4SC05317F","article-title":"Chemical diversity of reagents that modify RNA 2'-OH in water: a review","volume":"15","author":"Shioi","year":"2024","journal-title":"Chem Sci"},{"key":"2026051501515734700_rbag041-B137","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1007\/978-1-60761-670-2_3","article-title":"Amine coupling through EDC\/NHS: a practical approach","volume":"627","author":"Fischer","year":"2010","journal-title":"Methods Mol Biol"},{"key":"2026051501515734700_rbag041-B138"},{"key":"2026051501515734700_rbag041-B139","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.jconrel.2007.05.041","article-title":"Corneal gene therapy","volume":"124","author":"Klausner","year":"2007","journal-title":"J Control Release"},{"key":"2026051501515734700_rbag041-B140","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1038\/s41589-021-00770-1","article-title":"LYTACs that engage the asialoglycoprotein receptor for targeted protein degradation","volume":"17","author":"Ahn","year":"2021","journal-title":"Nat Chem Biol"},{"key":"2026051501515734700_rbag041-B141","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/j.jtos.2023.05.007","article-title":"Corneal gene therapy: structural and mechanistic understanding","volume":"29","author":"Kumar","year":"2023","journal-title":"Ocul Surf"},{"key":"2026051501515734700_rbag041-B142","first-page":"666","article-title":"Advances in gene therapy of ocular surface and corneal diseases","volume":"59","author":"Cong","year":"2023","journal-title":"Zhonghua Yan Ke Za Zhi"},{"key":"2026051501515734700_rbag041-B143","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1016\/j.jconrel.2025.02.030","article-title":"3D mesenchymal stem cell exosome-functionalized hydrogels for corneal wound healing","volume":"380","author":"Xu","year":"2025","journal-title":"J Control Release"},{"key":"2026051501515734700_rbag041-B144","doi-asserted-by":"crossref","first-page":"335","DOI":"10.3390\/pharmaceutics12040335","article-title":"Controlled release of rAAV vectors from APMA-functionalized contact lenses for corneal gene therapy","volume":"12","author":"Alvarez-Rivera","year":"2020","journal-title":"Pharmaceutics"},{"key":"2026051501515734700_rbag041-B145","first-page":"640","article-title":"MiRNA 24-3p-rich exosomes functionalized DEGMA-modified hyaluronic acid hydrogels for corneal epithelial healing","volume":"25","author":"Sun","year":"2023","journal-title":"Bioact Mater"},{"key":"2026051501515734700_rbag041-B146","doi-asserted-by":"crossref","first-page":"142013","DOI":"10.1016\/j.ijbiomac.2025.142013","article-title":"Composite synthetic protein hydrogel for inhibition of corneal fibrosis and treatment of corneal wounds","volume":"307","author":"Wang","year":"2025","journal-title":"Int J Biol Macromol"},{"key":"2026051501515734700_rbag041-B147","doi-asserted-by":"crossref","first-page":"1225","DOI":"10.1016\/j.ymthe.2023.01.021","article-title":"Extracellular vesicles: the next generation in gene therapy delivery","volume":"31","author":"Cecchin","year":"2023","journal-title":"Mol Ther"},{"key":"2026051501515734700_rbag041-B148","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1038\/nrm.2016.132","article-title":"Post-transcriptional gene regulation by mRNA modifications","volume":"18","author":"Zhao","year":"2017","journal-title":"Nat Rev Mol Cell Biol"},{"key":"2026051501515734700_rbag041-B149","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1038\/s41576-023-00611-y","article-title":"microRNAs in action: biogenesis, function and regulation","volume":"24","author":"Shang","year":"2023","journal-title":"Nat Rev Genet"},{"key":"2026051501515734700_rbag041-B150","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-1-0716-2823-2_1","article-title":"miRNA biogenesis and regulation of diseases: an updated overview","volume":"2595","author":"Vishnoi","year":"2023","journal-title":"Methods Mol Biol"},{"key":"2026051501515734700_rbag041-B151","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1186\/s13287-021-02697-9","article-title":"The application of mesenchymal stromal cells (MSCs) and their derivative exosome in skin wound healing: a comprehensive review","volume":"13","author":"Bian","year":"2022","journal-title":"Stem Cell Res Ther"},{"key":"2026051501515734700_rbag041-B152","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1007\/978-1-4615-0123-7_16","article-title":"Invasive drug delivery","volume":"513","author":"Blomer","year":"2002","journal-title":"Adv Exp Med Biol"},{"key":"2026051501515734700_rbag041-B153","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.actbio.2015.10.015","article-title":"Controlled release strategies for rAAV-mediated gene delivery","volume":"29","author":"Rey-Rico","year":"2016","journal-title":"Acta Biomater"},{"key":"2026051501515734700_rbag041-B154","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/j.ejpb.2014.12.022","article-title":"Treatment of ocular disorders by gene therapy","volume":"95","author":"Solin\u00eds","year":"2015","journal-title":"Eur J Pharm Biopharm"},{"key":"2026051501515734700_rbag041-B155","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1016\/j.brainresbull.2009.06.011","article-title":"Gene delivery to cornea","volume":"81","author":"Hao","year":"2010","journal-title":"Brain Res Bull"},{"key":"2026051501515734700_rbag041-B156","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1038\/s41563-024-01958-1","article-title":"Engineered nascent living human tissues with unit programmability","volume":"24","author":"Lavrador","year":"2025","journal-title":"Nat Mater"},{"key":"2026051501515734700_rbag041-B157","doi-asserted-by":"crossref","first-page":"S90","DOI":"10.1111\/epi.16539","article-title":"Precision medicine and therapies of the future","volume":"62","author":"Sisodiya","year":"2021","journal-title":"Epilepsia"},{"key":"2026051501515734700_rbag041-B158","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/j.jtos.2012.10.004","article-title":"Corneal gene therapy: basic science and translational perspective","volume":"11","author":"Mohan","year":"2013","journal-title":"Ocul Surf"},{"key":"2026051501515734700_rbag041-B159","doi-asserted-by":"crossref","first-page":"114721","DOI":"10.1016\/j.addr.2023.114721","article-title":"Nano-based eye drop: topical and noninvasive therapy for ocular diseases","volume":"194","author":"Wang","year":"2023","journal-title":"Adv Drug Deliv Rev"},{"key":"2026051501515734700_rbag041-B160","doi-asserted-by":"crossref","first-page":"4205","DOI":"10.1111\/bph.14024","article-title":"Principles of pharmacology in the eye","volume":"174","author":"Awwad","year":"2017","journal-title":"Br J Pharmacol"},{"key":"2026051501515734700_rbag041-B161","doi-asserted-by":"crossref","first-page":"816","DOI":"10.3390\/gels8120816","article-title":"Recent advances in hydrogels for the diagnosis and treatment of dry eye disease","volume":"8","author":"Li","year":"2022","journal-title":"Gels"},{"key":"2026051501515734700_rbag041-B162","doi-asserted-by":"crossref","first-page":"e2006335","DOI":"10.1002\/smll.202006335","article-title":"Hydrogels as emerging materials for cornea wound healing","volume":"17","author":"Khosravimelal","year":"2021","journal-title":"Small"},{"key":"2026051501515734700_rbag041-B163","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1038\/nn0901-877","article-title":"The fundamental plan of the retina","volume":"4","author":"Masland","year":"2001","journal-title":"Nat Neurosci"},{"key":"2026051501515734700_rbag041-B164","doi-asserted-by":"crossref","first-page":"e2306248","DOI":"10.1002\/advs.202306248","article-title":"A nucleic acid-based LYTAC plus platform to simultaneously mediate disease-driven protein downregulation","volume":"11","author":"Huang","year":"2024","journal-title":"Adv Sci (Weinh)"},{"key":"2026051501515734700_rbag041-B165","doi-asserted-by":"crossref","first-page":"e2402368","DOI":"10.1002\/advs.202402368","article-title":"A glucose-responsive hydrogel inhibits primary and secondary BRB injury for retinal microenvironment remodeling in diabetic retinopathy","volume":"11","author":"Zhou","year":"2024","journal-title":"Adv Sci (Weinh)"},{"key":"2026051501515734700_rbag041-B166","doi-asserted-by":"crossref","first-page":"101917","DOI":"10.1016\/j.mtbio.2025.101917","article-title":"Thermosensitive hydrogel composite with si-Cx43 nanoparticles and anti-VEGF agent for synergistic treatment of diabetic retinopathy","volume":"33","author":"Wen","year":"2025","journal-title":"Mater Today Bio"},{"key":"2026051501515734700_rbag041-B167","doi-asserted-by":"crossref","first-page":"114077","DOI":"10.1016\/j.jconrel.2025.114077","article-title":"Hyaluronic acid methacryloyl-based co-delivery system for aflibercept and miR-21-3p antagomir: a dual-therapeutic approach for diabetic retinopathy","volume":"386","author":"Ni","year":"2025","journal-title":"J Control Release"},{"key":"2026051501515734700_rbag041-B168","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1038\/s41580-023-00631-w","article-title":"Biology and therapeutic targeting of vascular endothelial growth factor A","volume":"24","author":"P\u00e9rez-Guti\u00e9rrez","year":"2023","journal-title":"Nat Rev Mol Cell Biol"},{"key":"2026051501515734700_rbag041-B169","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1210\/er.2003-0027","article-title":"Vascular endothelial growth factor: basic science and clinical progress","volume":"25","author":"Ferrara","year":"2004","journal-title":"Endocr Rev"},{"key":"2026051501515734700_rbag041-B170","first-page":"381","article-title":"Advancement of targeting therapy for retinal neovascularization","volume":"44","author":"Xiong","year":"2008","journal-title":"Zhonghua Yan Ke Za Zhi"},{"key":"2026051501515734700_rbag041-B171","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1007\/s10456-018-9618-5","article-title":"Gene therapy knockdown of VEGFR2 in retinal endothelial cells to treat retinopathy","volume":"21","author":"Simmons","year":"2018","journal-title":"Angiogenesis"},{"key":"2026051501515734700_rbag041-B172","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1038\/s41392-022-00966-4","article-title":"Targeted protein degradation: mechanisms, strategies and application","volume":"7","author":"Zhao","year":"2022","journal-title":"Signal Transduct Target Ther"},{"key":"2026051501515734700_rbag041-B173","doi-asserted-by":"crossref","first-page":"e202218106","DOI":"10.1002\/anie.202218106","article-title":"Aptamer-LYTACs for targeted degradation of extracellular and membrane proteins","volume":"62","author":"Wu","year":"2023","journal-title":"Angew Chem Int Ed Engl"},{"key":"2026051501515734700_rbag041-B174","doi-asserted-by":"crossref","first-page":"rbac056","DOI":"10.1093\/rb\/rbac056","article-title":"Recent advances in glucose-responsive insulin delivery systems: novel hydrogels and future applications","volume":"9","author":"Mohanty","year":"2022","journal-title":"Regen Biomater"},{"key":"2026051501515734700_rbag041-B175","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1097\/ICU.0000000000000143","article-title":"Advances in drug delivery to the posterior segment","volume":"26","author":"Pearce","year":"2015","journal-title":"Curr Opin Ophthalmol"},{"key":"2026051501515734700_rbag041-B176","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.preteyeres.2016.12.001","article-title":"Pharmacokinetic aspects of retinal drug delivery","volume":"57","author":"Del Amo","year":"2017","journal-title":"Prog Retin Eye Res"},{"key":"2026051501515734700_rbag041-B177","doi-asserted-by":"crossref","first-page":"e2302765","DOI":"10.1002\/smll.202302765","article-title":"Aflibercept loaded eye-drop hydrogel mediated with cell-penetrating peptide for corneal neovascularization treatment","volume":"20","author":"Zhou","year":"2024","journal-title":"Small"},{"key":"2026051501515734700_rbag041-B178"},{"key":"2026051501515734700_rbag041-B179","doi-asserted-by":"crossref","first-page":"105299","DOI":"10.1016\/j.ebiom.2024.105299","article-title":"Realising gene therapy: a biomedical challenge","volume":"106","author":"eBioMedicine","year":"2024","journal-title":"EBioMedicine"},{"key":"2026051501515734700_rbag041-B180","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1016\/j.ymthe.2021.01.013","article-title":"Three decades of clinical gene therapy: from experimental technologies to viable treatments","volume":"29","author":"Tremblay","year":"2021","journal-title":"Mol Ther"}],"container-title":["Regenerative Biomaterials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/rb\/advance-article-pdf\/doi\/10.1093\/rb\/rbag041\/67254151\/rbag041.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/rb\/article-pdf\/doi\/10.1093\/rb\/rbag041\/67254151\/rbag041.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/rb\/article-pdf\/doi\/10.1093\/rb\/rbag041\/67254151\/rbag041.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T05:52:23Z","timestamp":1778824343000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/rb\/article\/doi\/10.1093\/rb\/rbag041\/8508669"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":180,"URL":"https:\/\/doi.org\/10.1093\/rb\/rbag041","relation":{},"ISSN":["2056-3418","2056-3426"],"issn-type":[{"value":"2056-3418","type":"print"},{"value":"2056-3426","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026]]},"published":{"date-parts":[[2026]]},"article-number":"rbag041"}}