{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:08:27Z","timestamp":1792786107311,"version":"4.5.0"},"reference-count":48,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T00:00:00Z","timestamp":1786060800000},"content-version":"vor","delay-in-days":218,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Research Foundation of Korea","id":[{"id":"https:\/\/ror.org\/013aysd81","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Korea government","award":["RS-2026-25490015"],"award-info":[{"award-number":["RS-2026-25490015"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,1,22]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Organoids provide physiologically relevant platforms for disease modeling and drug screening, but their performance depends critically on the extracellular matrix (ECM). Matrigel, the current standard, suffers from undefined composition, batch-to-batch variability and limited tunability. We engineered a photo-crosslinkable hyaluronic acid\u2013collagen (HA\u2013collagen) composite hydrogel using riboflavin-mediated blue-light activation and examined how the collagen-to-HA ratio modulates physicochemical and biological properties. Storage modulus scaled with HA content (Col4HA1: 156\u2009Pa; Col3HA1: 216\u2009Pa; Col2HA1: 824\u2009Pa vs. 6\u2009Pa for Matrigel), and organoid formation efficiency was inversely correlated with stiffness: Col2HA1 yielded significantly fewer organoids than Matrigel, whereas Col4HA1 fell within a mechanically permissive range and supported organoid formation comparable to Matrigel. Col4HA1 retained 48.6\u2009\u00b1\u20092.3% of its mass throughout the 14-day organoid culture period, whereas Matrigel underwent complete degradation by Day 10. Organoids in Col4HA1 maintained stemness (Lgr5, Sox9) and differentiation markers (Villin), avoided stress-associated lysozyme elevation and preserved E-cadherin\/\u03b2-catenin junctions and epithelial polarity. Critically, Col4HA1 sustained stable Lgr5 expression across passages, whereas Matrigel showed passage-dependent Lgr5 elevation indicative of hyperproliferative drift. The photo-crosslinkable HA\u2013collagen hydrogel offers a chemically defined, tunable and reproducible Matrigel alternative for intestinal organoid research and regenerative-medicine applications.<\/jats:p>","DOI":"10.1093\/rb\/rbag171","type":"journal-article","created":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T11:49:25Z","timestamp":1786016965000},"source":"Crossref","is-referenced-by-count":0,"title":["A photo-crosslinkable hyaluronic acid\u2013collagen hydrogel maintains epithelial integrity and supports intestinal organoid stemness"],"prefix":"10.1093","volume":"13","author":[{"given":"Yeonoh","family":"Cho","sequence":"first","affiliation":[{"name":"Department of Food Science and Biotechnology, Gachon University , Seongnam-si 13120,","place":["Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Se Yeon","family":"Park","sequence":"additional","affiliation":[{"name":"School of Chemical, Biological and Battery Engineering, Gachon University , Seongnam-si 13120,","place":["Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8314-1981","authenticated-orcid":false,"given":"Dong-Wook","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Cogno-Mechatronics Engineering, Pusan National University , Busan 46241,","place":["Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyun Jong","family":"Lee","sequence":"additional","affiliation":[{"name":"School of Chemical, Biological and Battery Engineering, Gachon University , Seongnam-si 13120,","place":["Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3083-7595","authenticated-orcid":false,"given":"Jong Hun","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Food Science and Biotechnology, Gachon University , Seongnam-si 13120,","place":["Republic of 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