{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T04:00:45Z","timestamp":1781150445729,"version":"3.54.1"},"reference-count":43,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82371681"],"award-info":[{"award-number":["82371681"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U24A20658"],"award-info":[{"award-number":["U24A20658"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Hubei Provincial Natural Science Foundation of China","award":["2024AFB675"],"award-info":[{"award-number":["2024AFB675"]}]},{"DOI":"10.13039\/501100012166","name":"National Key R&D Program of China","doi-asserted-by":"publisher","award":["2023YFC2705505"],"award-info":[{"award-number":["2023YFC2705505"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,6,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>In brief: Aberrant embryo\u2013maternal cross-talk is a potential cause of in vitro fertilization failure. This study reveals that embryo-derived exosomes may carry specific microRNAs that impair endometrial receptivity.<\/jats:p>\n                  <jats:p>Abstract: Despite morphological selection, \u223c40% of transferable embryos fail to implant during in vitro fertilization, potentially due to aberrant embryo\u2013maternal cross-talk. Pre-implantation embryos release extracellular vesicles into their microenvironment, which may participate in embryo\u2013maternal communication. This study investigated the microRNA expression profiles of extracellular vesicles derived from pre-implantation human embryos and analyzed their functional effects on the decidualization of endometrial stromal cells. Extracellular vesicles were isolated from the spent culture media of day 5 blastocysts associated with either successful clinical pregnancy or implantation failure. Sequencing and quantitative reverse transcription-PCR were performed to identify and validate differentially expressed microRNAs. Functional effects were assessed by measuring decidualization markers, while regulatory mechanisms were evaluated via western blotting. We found that miR-143-3p expression was significantly upregulated in blastocyst-derived extracellular vesicles from the implantation failure group. Mechanistically, miR-143-3p hinders the decidualization process of endometrial stromal cells by targeting Kirsten rat sarcoma viral oncogene homolog to inhibit its expression and suppress the downstream extracellular regulated protein kinase\/protein kinase B signaling pathway. This study provides the first characterization of microRNA expression profiles in extracellular vesicles derived from pre-implantation embryos of in vitro fertilization patients, revealing a novel mechanism in embryo\u2013maternal cross-talk. Our findings suggest that exosomal miR-143-3p acts as a negative regulator of decidualization via the Kirsten rat sarcoma viral oncogene homolog\u2013extracellular regulated protein kinase\/protein kinase B axis and may serve as a promising biomarker for the non-invasive assessment of embryo implantation potential.<\/jats:p>","DOI":"10.1093\/reprod\/xaag052","type":"journal-article","created":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T12:14:44Z","timestamp":1777637684000},"source":"Crossref","is-referenced-by-count":0,"title":["Human embryo exosomal miR-143-3p impairs decidualization by targeting KRAS"],"prefix":"10.1093","volume":"171","author":[{"given":"Huajing","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology , 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Wuhan,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiangtian","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology , Wuhan,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erqing","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology , Wuhan,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofang","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology , Wuhan,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology , Wuhan,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Defeng","family":"Shu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology , Wuhan,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology , Wuhan,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4403-2325","authenticated-orcid":false,"given":"Yi","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology , Wuhan,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7997-4358","authenticated-orcid":false,"given":"Ling","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology , 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