{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:33:41Z","timestamp":1787009621205,"version":"build-2736575974"},"reference-count":40,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82502009"],"award-info":[{"award-number":["82502009"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Key Research and Development Project of China","award":["2024YFC2706701"],"award-info":[{"award-number":["2024YFC2706701"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,6,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>In brief: Human fallopian tube extracellular vesicles (EVs) enhance preimplantation embryo development by delivering miR-21-5p, which suppresses PTEN\/p53-dependent apoptosis and promotes blastocyst formation. Restoring this physiological miRNA signal may offer a biomimetic strategy to optimize embryo culture in in-vitro fertilization.<\/jats:p>\n                  <jats:p>Abstracts: Extracellular vesicles (EVs) released by the maternal reproductive tract provide essential biochemical support for early embryo development, yet the identity of their functional molecular cargo remains largely unclear. In this study, we isolated and characterized human fallopian tube\u2013derived EVs (oviductal EVs, oEVs) and demonstrated that they are efficiently internalized by preimplantation embryos. Supplementation with oEVs enhanced blastocyst formation and reduced apoptosis without impairing implantation or postimplantation development, suggesting a physiological role for oEV signals in sustaining embryo viability. To identify candidate effector molecules, we performed cross-dataset analysis of reproductive EV miRNA profiles and highlighted miR-21-5p as a conserved and abundant candidate. In vivo vs in vitro expression comparison revealed that miR-21-5p is markedly depleted in in vitro\u2013cultured embryos from the 4-cell stage onward. Supplementation with miR-21-5p recapitulated the developmental benefits of oEVs, including improved blastocyst progression and reduced apoptosis. Transcriptomic profiling further revealed suppression of PTEN\/p53-mediated stress-response and apoptotic pathways, supporting a mechanistic link between maternal EV-derived miRNAs and enhanced embryonic resilience under culture conditions. Together, these findings identify miR-21-5p as a functional mediator of oEV-driven embryo support, reveal a nanoscale maternal\u2013embryo communication mechanism, and suggest potential translational strategies using miRNA-based supplements or EV-mimetic nanocarriers to improve assisted reproductive technologies.<\/jats:p>","DOI":"10.1093\/reprod\/xaag065","type":"journal-article","created":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T11:44:29Z","timestamp":1779450269000},"source":"Crossref","is-referenced-by-count":1,"title":["Functional identification of miR-21-5p from fallopian tubal extracellular vesicles as a promoter of preimplantation embryo viability"],"prefix":"10.1093","volume":"171","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3244-0353","authenticated-orcid":false,"given":"Yuehan","family":"Li","sequence":"first","affiliation":[{"name":"Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology , 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