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This study demonstrates that rHSPA5 enhances oocyte mitochondrial activity and modulates gene expression related to embryonic development, thereby improving blastocyst formation rates.<\/jats:p>\n                  <jats:p>Abstract: Oviductal fluid (OF) proteins, such as heat shock protein A5 (HSPA5), play essential roles in reproductive physiology, including zona pellucida (ZP)\u2013sperm interactions. Hypothesizing that HSPA5 interacts with oocytes, we evaluated its effects during the last 4\u2009hr of bovine in-vitro cumulus\u2013oocyte complex (COC) maturation and subsequent embryo development. The COCs were aspirated from slaughterhouse-derived cow ovaries and matured for 20\u2009hr in base medium (BM). The medium was then partially replaced with BM (control) or BM supplemented with recombinant HSPA5 (100\u2009ng\/ml) for the final 4\u2009hr of maturation. After maturation, COCs were either denuded for analysis or used for in-vitro fertilization and culture to assess embryo development to the blastocyst stage. To investigate HSPA5\u2019s role, we assessed the mitochondrial membrane potential, reactive oxygen species, and glutathione content in COCs and maturation medium, ZP solubility, oocyte penetration, blastocyst rates, and gene expression. Recombinant HSPA5 (rHSPA5) enhanced oocyte mitochondrial activity (p\u2009=\u20090.0006) and significantly increased the blastocyst yield (p\u2009=\u20090.04). Blastocysts from rHSPA5-treated oocytes showed upregulation of 19 genes associated with embryo quality, DNA methylation, cell growth, development, and oxidative stress. Although rHSPA5 did not alter the monospermic fertilization rate or oxidative stress in oocytes, it improved mitochondrial function, supported blastocyst development, and modulated embryonic transcriptional profiles. These findings suggest that rHSPA5 plays a beneficial role during the final phase of oocyte maturation, contributing to improved embryo quality and developmental competence in vitro.<\/jats:p>","DOI":"10.1093\/reprod\/xaag070","type":"journal-article","created":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T15:33:07Z","timestamp":1782487987000},"source":"Crossref","is-referenced-by-count":0,"title":["Evidence that heat shock protein A5 (HSPA5) plays a role during bovine in vitro embryo production"],"prefix":"10.1093","volume":"172","author":[{"given":"Priscila Helena","family":"Santos","sequence":"first","affiliation":[{"name":"Institute of Bioscience, S\u00e3o Paulo State University (Unesp) , Botucatu, S\u00e3o Paulo,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda Fagali","family":"Franchi","sequence":"additional","affiliation":[{"name":"Institute of Bioscience, S\u00e3o Paulo State University (Unesp) , Botucatu, S\u00e3o 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Valencise","family":"Quaglio","sequence":"additional","affiliation":[{"name":"Institute of Bioscience, S\u00e3o Paulo State University (Unesp) , Botucatu, S\u00e3o Paulo,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo F\u00e1bio Gouveia","family":"Nogueira","sequence":"additional","affiliation":[{"name":"Biological Sciences, S\u00e3o Paulo State University (Unesp) , Assis, S\u00e3o Paulo,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1666-7021","authenticated-orcid":false,"given":"Anthony C\u00e9sar de Souza","family":"Castilho","sequence":"additional","affiliation":[{"name":"University of Western S\u00e3o Paulo (Unoeste) , Presidente Prudente, S\u00e3o 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