{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T06:49:26Z","timestamp":1791874166928,"version":"4.3.4"},"reference-count":0,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T00:00:00Z","timestamp":1791763200000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>NAD+ is an important regulator of cellular physiological processes. However, in the culture environment, NAD+ levels are predicted to decrease below physiological requirements. Therefore, we examined the effects of the NAD+ precursor nicotinamide mononucleotide (NMN) on NAD+ levels and viability of mammalian fertilized ova. NAD+ levels decreased during in vitro culture, but this decrease was alleviated by NMN. NMN also increased the blastocyst formation rate and accelerated the developmental rate. Transcriptome analysis showed that NMN induced significant changes in the expression of genes involved in mitochondrial function in morula, and that NMN increased mitochondrial membrane potential, oxygen consumption rate and mitochondrial DNA copy number of morula while reducing reactive oxygen species levels.<\/jats:p>\n                  <jats:p>The addition of NMN to the embryo culture medium increased NAD+ levels in embryos, boosted mitochondrial function at the morula stage, and enhanced their ability to develop into blastocysts.<\/jats:p>","DOI":"10.1093\/reprod\/xaag128","type":"journal-article","created":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T11:25:46Z","timestamp":1791804346000},"source":"Crossref","is-referenced-by-count":0,"title":["Nicotinamide mononucleotide supports the development of bovine embryo through enhancement of mitochondrial function in morula"],"prefix":"10.1093","author":[{"given":"Shuting","family":"Ye","sequence":"first","affiliation":[{"name":"Osaka Metropolitan University Graduate School of Medicine, , Osaka 545-8585,","place":["Japan"]},{"name":"Osaka Metropolitan University Women\u2019s Life Care Medicine, School of Medicine, , Osaka, 545-8585,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9083-1403","authenticated-orcid":false,"given":"Shu","family":"Hashimoto","sequence":"additional","affiliation":[{"name":"Osaka Metropolitan University Graduate School of Medicine, , Osaka 545-8585,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaya","family":"Yamanaka","sequence":"additional","affiliation":[{"name":"HORAC Grand Front Osaka Clinic , Osaka 530-0011,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2198-1069","authenticated-orcid":false,"given":"Sanath Udayanga Kankanam","family":"Gamage","sequence":"additional","affiliation":[{"name":"HORAC Grand Front Osaka Clinic , Osaka 530-0011,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuki","family":"Inoue","sequence":"additional","affiliation":[{"name":"Tokyo University of Agriculture Department of Animal Science, , Kanagawa 243-0034,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hisataka","family":"Iwata","sequence":"additional","affiliation":[{"name":"Tokyo University of Agriculture Department of Animal Science, , Kanagawa 243-0034,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2834-2726","authenticated-orcid":false,"given":"Yoshiharu","family":"Morimoto","sequence":"additional","affiliation":[{"name":"HORAC Grand Front Osaka Clinic , Osaka 530-0011,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daisuke","family":"Tachibana","sequence":"additional","affiliation":[{"name":"Osaka Metropolitan University Women\u2019s Life Care Medicine, School of Medicine, , Osaka, 545-8585,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,10,12]]},"container-title":["Reproduction"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/reproduction\/advance-article-pdf\/doi\/10.1093\/reprod\/xaag128\/71892550\/xaag128.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/reproduction\/advance-article-pdf\/doi\/10.1093\/reprod\/xaag128\/71892550\/xaag128.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T06:12:45Z","timestamp":1791871965000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/reproduction\/advance-article\/doi\/10.1093\/reprod\/xaag128\/8911850"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,12]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1093\/reprod\/xaag128","relation":{},"ISSN":["1470-1626","1741-7899"],"issn-type":[{"value":"1470-1626","type":"print"},{"value":"1741-7899","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,12]]},"article-number":"xaag128"}}