{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T10:18:38Z","timestamp":1778840318294,"version":"3.51.4"},"reference-count":43,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2018,8,2]],"date-time":"2018-08-02T00:00:00Z","timestamp":1533168000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"ORTON Orthopaedic Hospital"},{"DOI":"10.13039\/100010135","name":"Finnish Medical Society","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100010135","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100007797","name":"University of Helsinki","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007797","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Maire Lisko Foundation"},{"name":"Finnish Society for Rheumatology"},{"DOI":"10.13039\/100008376","name":"Helsinki University Central Hospital","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008376","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objective<\/jats:title>\n                  <jats:p>In this work, we aimed to elucidate the molecular mechanisms driving primary OA. By studying the dynamics of protein expression in two different types of OA joints we searched for similarities and disparities to identify key molecular mechanisms driving OA.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>For this purpose, human SF samples were obtained from CMC-I OA and knee joint of OA patients. SF samples were analysed by label-free quantitative liquid chromatography mass spectrometry. Disease-relevant proteins identified in proteomics studies, such as clusterin, paraoxonase\/arylesterase 1 (PON1) and transthyretin were validated by enzyme-linked immunosorbent assays, and on the mRNA level by droplet digital PCR. Functional studies were performed in vitro using primary chondrocytes.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Differential proteomic changes were observed in the concentration of 40 proteins including clusterin, PON1 and transthyretin. Immunoassay analyses of clusterin, PON1, transthyretin and other inflammatory cytokines confirmed significant differences in protein concentration in SF of CMC-I and knee OA patients, with primarily lower protein expression levels in CMC-I. Functional studies on chondrocytes unequivocally demonstrated that stimulation with SF obtained from knee OA, in contrast to CMC-I OA joint, caused a significant upregulation in pro-inflammatory response, cell death and hypertrophy.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>This study demonstrates that differential expression of molecular players in SF from different OA joints evokes diverse effects on primary chondrocytes. The pathomolecular mechanisms of OA may significantly differ in various joints, a finding that brings a new dimension into the pathogenesis of primary OA.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/rheumatology\/key232","type":"journal-article","created":{"date-parts":[[2018,7,6]],"date-time":"2018-07-06T01:21:05Z","timestamp":1530840065000},"page":"897-907","source":"Crossref","is-referenced-by-count":11,"title":["Functional analysis of synovial fluid from osteoarthritic knee and carpometacarpal joints unravels different molecular profiles"],"prefix":"10.1093","volume":"58","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4444-9208","authenticated-orcid":false,"given":"Goncalo","family":"Barreto","sequence":"first","affiliation":[{"name":"Tissue Engineering + Biofabrication, ETH Z\u00fcrich, Z\u00fcrich, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rabah","family":"Soliymani","sequence":"additional","affiliation":[{"name":"Helsinki Institute for Life Science (HiLIFE) and Faculty of Medicine, Biochemistry\/Developmental Biology, Meilahti Clinical Proteomics Core Facility, University of Helsinki, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc","family":"Baumann","sequence":"additional","affiliation":[{"name":"Helsinki Institute for Life Science (HiLIFE) and Faculty of Medicine, Biochemistry\/Developmental Biology, Meilahti Clinical Proteomics Core Facility, University of Helsinki, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eero","family":"Waris","sequence":"additional","affiliation":[{"name":"Department of Hand Surgery, University of Helsinki and Helsinki University Central Hospital, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kari K","family":"Eklund","sequence":"additional","affiliation":[{"name":"Rheumatology, University of Helsinki and Helsinki University Central Hospital, Finland"},{"name":"Orton Orthopaedic Hospital and Research Institute, Invalid Foundation, Helsinki, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcy","family":"Zenobi-Wong","sequence":"additional","affiliation":[{"name":"Tissue Engineering + Biofabrication, ETH Z\u00fcrich, Z\u00fcrich, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maciej","family":"Lalowski","sequence":"additional","affiliation":[{"name":"Helsinki Institute for Life Science (HiLIFE) and Faculty of Medicine, Biochemistry\/Developmental Biology, Meilahti Clinical Proteomics Core Facility, University of Helsinki, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2018,8,2]]},"reference":[{"key":"2019081609501116400_key232-B1","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1097\/BOR.0b013e328349c2d2","article-title":"Imaging of osteoarthritis: update from a radiological perspective","volume":"23","author":"Guermazi","year":"2011","journal-title":"Curr Opin Rheumatol"},{"key":"2019081609501116400_key232-B2","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1136\/ard.2005.037275","article-title":"Molecular markers of cartilage breakdown and synovitis at baseline as predictors of structural progression of hip osteoarthritis. 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