{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,19]],"date-time":"2023-09-19T13:23:51Z","timestamp":1695129831260},"reference-count":37,"publisher":"Oxford University Press (OUP)","issue":"14","license":[{"start":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T00:00:00Z","timestamp":1693526400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,9,18]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The objective was to investigate the influence of different pre-storage temperatures in the dicentric chromosome analysis (DCA) protocol (22\u00b0C vs. 37\u00b0C) by using \u03b3-H2AX\u00a0+\u00a053BP1 foci as a marker for deoxyribonucleic acid (DNA) double-strand break (DSB) damage induction and repair and the formation of dicentric chromosomes as a result of mis-repair. Repair of \u03b3-H2AX\u00a0+\u00a053BP1 DSB foci was absent in samples that were incubated for 2\u00a0h at 22\u00b0C after exposure of 0.5 and 1.2\u00a0Gy. When 0.5- and 1.2-Gy-exposed samples were incubated at 37\u00b0C for 2\u00a0h, there was an average decline of 31 and 52% of DSB foci, respectively. This indicated that DNA repair occurred. There was a 27% decrease in dicentric chromosome yield at 1.2\u00a0Gy and a 15% decrease at 3.5\u00a0Gy after post-irradiation incubation for 2 h at 37\u00b0C relative to the observed dicentric frequencies at 22\u00b0C. Recommended to re-phase: our data suggested that there were more open DSBs after a 2-h incubation at 22\u00b0C, which contributed to more mis-repair and dicentric formation from the start of culture. Our findings are corroborated by publications showing that lesion interaction based on enzymatic activity is suppressed below 21\u00b0C. As such temperature variations can be a source of variation in DCA during interlaboratory comparison studies, we propose to establish a common guide for the standardisation of pre-culture conditions in cytogenetic dosimetry proficiency testing.<\/jats:p>","DOI":"10.1093\/rpd\/ncad128","type":"journal-article","created":{"date-parts":[[2023,5,24]],"date-time":"2023-05-24T21:54:43Z","timestamp":1684965283000},"page":"1485-1494","source":"Crossref","is-referenced-by-count":0,"title":["Postirradiation temperature influences DSB repair and dicentric chromosome formation\u2014potential impact for dicentric chromosome analysis in interlaboratory comparisons"],"prefix":"10.1093","volume":"199","author":[{"given":"Christina","family":"Beinke","sequence":"first","affiliation":[{"name":"Bundeswehr Institute of Radiobiology, affiliated to the University of Ulm , Neuherbergstr.11, D-80937 Munich , Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthias","family":"Port","sequence":"additional","affiliation":[{"name":"Bundeswehr Institute of Radiobiology, affiliated to the University of Ulm , Neuherbergstr.11, D-80937 Munich , Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harry","family":"Scherthan","sequence":"additional","affiliation":[{"name":"Bundeswehr Institute of Radiobiology, affiliated to the University of Ulm , Neuherbergstr.11, D-80937 Munich , Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2023,9,18]]},"reference":[{"issue":"1","key":"2023091807460337900_ref1","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1093\/rpd\/ncy137","article-title":"Biodosimetry and biodosimetry networks for managing radiation emergency","volume":"182","author":"Kulka","year":"2018","journal-title":"Radiat. 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