{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T06:33:35Z","timestamp":1791959615661,"version":"4.3.4"},"reference-count":15,"publisher":"Oxford University Press (OUP)","issue":"13","license":[{"start":{"date-parts":[[2023,7,13]],"date-time":"2023-07-13T00:00:00Z","timestamp":1689206400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100004748","name":"Mashhad University of Medical Sciences, Iran","doi-asserted-by":"crossref","award":["981576"],"award-info":[{"award-number":["981576"]}],"id":[{"id":"10.13039\/501100004748","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,8,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Radiation during radiotherapy and nuclear accidents is currently one of the biggest concerns for the international community. Biological dosimetry examines the amount of damage caused by radiation at the cellular level by quantifying a radiation biomarker. In particular, the dicentric chromosome assay is a biodosimetric technique that can quantify radiation damage by correlating radiation dose exposure with the frequency of dicentric chromosomes in the peripheral lymphocytes extracted from exposed individuals. This study aims to present of the reference dose\u2013response calibration curve for biodosimetry laboratory of Mashhad University of Medical Sciences (north-east of Iran). In all, 40 samples of peripheral blood from four healthy volunteers were irradiated at doses of 0\u20135 Gray in a customised water phantom using a 6\u00a0MV X-rays at dose rate of 2\u00a0Gy\/min from a linear accelerator. The irradiated samples were cultured and analysed according to the International Atomic Energy Agency Cytogenetic Dosimetry Protocol (2011) with some modifications. Linear-quadratic model curve fitting and further statistical analysis were done using Chromosome Aberration Calculation Software Version 2.0 and Dose Estimate (Version 5.2). The curve equation obtained was ${Y}_{dic}=0.0533{D}^2+0.0231D+0.0001$ and was in the range of other studies. Validation of the calibration curve was done by estimating the dose of blind samples.<\/jats:p>","DOI":"10.1093\/rpd\/ncad200","type":"journal-article","created":{"date-parts":[[2023,7,14]],"date-time":"2023-07-14T06:15:05Z","timestamp":1689315305000},"page":"1410-1415","source":"Crossref","is-referenced-by-count":4,"title":["Establishment and validation of a calibration curve for dicentric chromosome induced by 6MV X-ray"],"prefix":"10.1093","volume":"199","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3352-863X","authenticated-orcid":false,"given":"Morteza","family":"Kosarnia","sequence":"first","affiliation":[{"name":"Mashhad University of Medical Sciences Department of Medical Physics, Faculty of Medicine, , Mashhad 9138813944 , Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad-Taghi","family":"Bahreyni-Toossi","sequence":"additional","affiliation":[{"name":"Mashhad University of Medical Sciences Medical Physics Research Center, , Mashhad 9138813944 , Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2246-6939","authenticated-orcid":false,"given":"Hamid","family":"Gholamhosseinian","sequence":"additional","affiliation":[{"name":"Mashhad University of Medical Sciences Medical Physics Research Center, , Mashhad 9138813944 , Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elham","family":"Dolat","sequence":"additional","affiliation":[{"name":"Mashhad University of Medical Sciences Department of Medical Physics, Faculty of Medicine, , Mashhad 9138813944 , Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Parisa","family":"Fakour-Mollaee","sequence":"additional","affiliation":[{"name":"Mashhad University of Medical Sciences Department of Medical Physics, Faculty of Medicine, , Mashhad 9138813944 , Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2951-8228","authenticated-orcid":false,"given":"Hosein","family":"Azimian","sequence":"additional","affiliation":[{"name":"Mashhad University of Medical Sciences Medical Physics Research Center, , Mashhad 9138813944 , Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,7,13]]},"reference":[{"key":"2023080913340021000_ref1","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1097\/HP.0b013e3181b35a53","article-title":"Establishment of an x-ray standard calibration curve by conventional dicentric analysis as prerequisite for accurate radiation dose assessment","volume":"98","author":"Beinke","year":"2010","journal-title":"Health Phys."},{"key":"2023080913340021000_ref2","doi-asserted-by":"crossref","first-page":"908","DOI":"10.1590\/1414-431x20154470","article-title":"A dose-response curve for biodosimetry from a 6 MV electron linear accelerator","volume":"48","author":"Lemos-Pinto","year":"2015","journal-title":"Braz. 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Biophys."},{"key":"2023080913340021000_ref4","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1089\/hs.2017.0050","article-title":"Biodosimetry: a future tool for Medical Management of Radiological Emergencies","volume":"15","author":"Sproull","year":"2017","journal-title":"Health Secur."},{"key":"2023080913340021000_ref5","volume-title":"Cytogenetic Dosimetry: Applications in Preparedness for and Response to Radiation Emergencies","author":"IAEA","year":"2011"},{"key":"2023080913340021000_ref6","doi-asserted-by":"crossref","first-page":"7","DOI":"10.4103\/2041-9414.197162","article-title":"Establishment of a dose-response curve for X-ray-induced micronuclei in human lymphocytes","volume":"7","author":"Lusiyanti","year":"2016","journal-title":"Genome Integr."},{"key":"2023080913340021000_ref7","doi-asserted-by":"crossref","first-page":"2","DOI":"10.4103\/genint.genint_1_19","article-title":"Development of dose-response calibration curve for dicentric chromosome induced by X-rays","volume":"10","author":"Lusiyanti","year":"2019","journal-title":"Genome Integr."},{"key":"2023080913340021000_ref8","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1097\/HP.0b013e3181ab3ccf","article-title":"The use of the dicentric assay for biological dosimetry for radiation accidents in Bulgaria","volume":"98","author":"Hadjidekova","year":"2010","journal-title":"Health Phys."},{"key":"2023080913340021000_ref9","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1080\/09553009214551021","article-title":"Chromosomal aberrations in human lymphocytes induced in vitro by very low doses of X-rays","volume":"61","author":"Lloyd","year":"1992","journal-title":"Int. J. Radiat. Biol."},{"key":"2023080913340021000_ref10","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.mrgentox.2012.09.009","article-title":"Implementation of a dose-response curve for \u03b3-radiation in the Portuguese population by use of the chromosomal aberration assay","volume":"750","author":"Martins","year":"2013","journal-title":"Mutat. Res."},{"key":"2023080913340021000_ref11","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/S1383-5718(02)00067-0","article-title":"Chromosome aberrations induced in human lymphocytes by in vitro and in vivo X-rays","volume":"517","author":"Schr\u00f6der","year":"2002","journal-title":"Mutat. 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