{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T03:38:16Z","timestamp":1782790696927,"version":"3.54.5"},"reference-count":20,"publisher":"Oxford University Press (OUP)","issue":"19","license":[{"start":{"date-parts":[[2023,9,14]],"date-time":"2023-09-14T00:00:00Z","timestamp":1694649600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Environmental Radioactivity Research Network Center","award":["Grant Number: I-22-19"],"award-info":[{"award-number":["Grant Number: I-22-19"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,11,16]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>In the event of a radiological incident, a fast and accurate biological dosimetry (biodosimetry) method for evaluating people who have been potentially exposed to ionising radiation is crucial. Among the many biodosimetry methods available, the immunodetection of phosphorylated H2AX (\u03b3-H2AX) stands as a promising method to be used in the triage of patients exposed to radiation. Currently, the most common way to measure \u03b3-H2AX levels is through fluorescence microscopy. In this pilot study, we assessed the feasibility of using an enzyme-linked immunosorbent assay (ELISA) for quantifying \u03b3-H2AX for biodosimetry purposes. Moreover, the usefulness of measuring phosphorylated ATM (pATM) levels through ELISA for biodosimetry was also evaluated. Blood samples were obtained from three male donors (38\u00a0y) and were irradiated with 60Co (0, 1, 2 and 6\u00a0Gy). Peripheral blood mononuclear cells (PBMCs) were isolated and lysed before measuring \u03b3-H2AX, total H2AX protein and pATM using ELISA kits. The dicentric chromosome assay (DCA) using whole blood was also performed for comparison. Data from all donors at each dose were pooled before statistical analysis. The ratio of \u03b3-H2AX\/total H2AX and pATM levels increased in a radiation-dose-dependent manner. The average \u03b3-H2AX\/total H2AX ratios were 0.816\u00a0\u00b1\u00a00.219, 0.830\u00a0\u00b1\u00a00.685, 1.276\u00a0\u00b1\u00a01.151 and 1.606\u00a0\u00b1\u00a01.098, whereas the average levels of pATM were 59.359\u00a0\u00b1\u00a03.740, 63.366\u00a0\u00b1\u00a00.840, 66.273\u00a0\u00b1\u00a02.603 and 69.936\u00a0\u00b1\u00a04.439, in PBMCs exposed to 0, 1, 2 and 6\u00a0Gy, respectively. The linear-quadratic dose\u2013response calibration curve for DCA was Y\u00a0=\u00a00.0017 (\u00b10.0010)\u00a0+\u00a00.0251 (\u00b10.0142)\u00a0\u00d7\u00a0D\u00a0+\u00a00.0342 (\u00b10.0039)\u00a0\u00d7\u00a0D2 \u00a0$\\boldsymbol{Y}=\\mathbf{0.0017}\\left(\\pm \\mathbf{0.0010}\\right)+\\mathbf{0.0208}\\left(\\pm \\mathbf{0.0218}\\right)\\times \\boldsymbol{D}+\\mathbf{0.0350}\\left(\\pm \\mathbf{0.0050}\\right)\\times{\\boldsymbol{D}}^{\\mathbf{2}}$. Overall, despite a large variability in the ratio of \u03b3-H2AX\/total H2AX among donors, the present study revealed the suitability of using the ratio of \u03b3-H2AX\/total H2AX and pATM for biodosimetry. Still, more research with a larger group of subjects is necessary to construct a reliable calibration curve for the ratio of \u03b3-H2AX\/total H2AX and pATM levels for biodosimetry.<\/jats:p>","DOI":"10.1093\/rpd\/ncad253","type":"journal-article","created":{"date-parts":[[2023,9,15]],"date-time":"2023-09-15T14:00:28Z","timestamp":1694786428000},"page":"2383-2390","source":"Crossref","is-referenced-by-count":4,"title":["\u03b3-H2AX and phospho-ATM enzyme-linked immunosorbent assays as biodosimetry methods for radiation exposure assessment: a pilot study"],"prefix":"10.1093","volume":"199","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3018-2485","authenticated-orcid":false,"given":"Dwi","family":"Ramadhani","sequence":"first","affiliation":[{"name":"Research Organization for Nuclear Energy, National Research and Innovation Agency Research Center for Radioisotope, Radiopharmaceutical and Biodosimetry Technology, , Gedung 720 KST. BJ. Habibie, Jl. Raya Puspiptek 60, Tangerang Selatan, Banten 15310 , Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Devita","family":"Tetriana","sequence":"additional","affiliation":[{"name":"Research Organization for Nuclear Energy, National Research and Innovation Agency Research Center for Safety, Metrology and Nuclear Quality Technology, , Gedung 720 KST. BJ. Habibie, Jl. Raya Puspiptek 60, Tangerang Selatan, Banten 15310 , Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sofiati","family":"Purnami","sequence":"additional","affiliation":[{"name":"Research Organization for Nuclear Energy, National Research and Innovation Agency Research Center for Safety, Metrology and Nuclear Quality Technology, , Gedung 720 KST. BJ. Habibie, Jl. Raya Puspiptek 60, Tangerang Selatan, Banten 15310 , Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viria Agesti","family":"Suvifan","sequence":"additional","affiliation":[{"name":"Deputy for Research and Innovation Infrastructure, National Research and Innovation Agency Directorate of Strengthening and Partnership of Research and Innovation Infrastructure, , Jl. M.H.Thamrin No.8, Jakarta Pusat 10340 , Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iin","family":"Kurnia Hasan Basri","sequence":"additional","affiliation":[{"name":"Research Organization for Nuclear Energy, National Research and Innovation Agency Research Center for Radioisotope, Radiopharmaceutical and Biodosimetry Technology, , Gedung 720 KST. BJ. Habibie, Jl. Raya Puspiptek 60, Tangerang Selatan, Banten 15310 , Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teja","family":"Kisnanto","sequence":"additional","affiliation":[{"name":"Research Organization for Nuclear Energy, National Research and Innovation Agency Research Center for Radioisotope, Radiopharmaceutical and Biodosimetry Technology, , Gedung 720 KST. BJ. Habibie, Jl. Raya Puspiptek 60, Tangerang Selatan, Banten 15310 , Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiara Andalya","family":"Oktariyani","sequence":"additional","affiliation":[{"name":"Al-Azhar University of Indonesia Department of Biology, Faculty of Science and Technology, , Komplek Masjid Agung Al Azhar Jl. Sisingamangaraja, Jakarta Selatan, Kebayoran Baru 12110 , Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dira","family":"Syafira","sequence":"additional","affiliation":[{"name":"Al-Azhar University of Indonesia Department of Biology, Faculty of Science and Technology, , Komplek Masjid Agung Al Azhar Jl. Sisingamangaraja, Jakarta Selatan, Kebayoran Baru 12110 , Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhamad Yasin","family":"Yunus","sequence":"additional","affiliation":[{"name":"National Research and Innovation Agency Research Center for Radiation Process Technology, Research Organization for Nuclear Energy, , Gedung 720 KST. BJ. Habibie, Jl. Raya Puspiptek 60, Tangerang Selatan, Banten 15310 , Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3104-2134","authenticated-orcid":false,"given":"Tomisato","family":"Miura","sequence":"additional","affiliation":[{"name":"Institute of Radiation Emergency Medicine, Hirosaki University , Hirosaki, Aomori 036-8564 , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mukh","family":"Syaifudin","sequence":"additional","affiliation":[{"name":"Research Organization for Nuclear Energy, National Research and Innovation Agency Research Center for Radioisotope, Radiopharmaceutical and Biodosimetry Technology, , Gedung 720 KST. BJ. Habibie, Jl. Raya Puspiptek 60, Tangerang Selatan, Banten 15310 , Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Retno","family":"Widowati","sequence":"additional","affiliation":[{"name":"Universitas Nasional Department of Biology, , Jl. Sawo Manila, Pejaten Ps. Minggu, Jakarta Selatan 12520 , Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,9,14]]},"reference":[{"key":"2023111615363343900_ref1","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/j.mrgentox.2013.04.002","article-title":"Analysis of chromosome damage for biodosimetry using imaging flow cytometry","volume":"756","author":"Beaton","year":"2013","journal-title":"Mutat. Res."},{"key":"2023111615363343900_ref2","doi-asserted-by":"crossref","first-page":"159","DOI":"10.3857\/roj.2021.00339","article-title":"Cytogenetic biological dosimetry assays: recent developments and updates","volume":"39","author":"Gnanasekaran","year":"2021","journal-title":"Radiat. Oncol. 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Res."}],"container-title":["Radiation Protection Dosimetry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/rpd\/article-pdf\/199\/19\/2383\/53456668\/ncad253.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/rpd\/article-pdf\/199\/19\/2383\/53456668\/ncad253.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,16]],"date-time":"2023-11-16T16:52:28Z","timestamp":1700153548000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/rpd\/article\/199\/19\/2383\/7273864"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9,14]]},"references-count":20,"journal-issue":{"issue":"19","published-online":{"date-parts":[[2023,9,14]]},"published-print":{"date-parts":[[2023,11,16]]}},"URL":"https:\/\/doi.org\/10.1093\/rpd\/ncad253","relation":{},"ISSN":["0144-8420","1742-3406"],"issn-type":[{"value":"0144-8420","type":"print"},{"value":"1742-3406","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2023,11]]},"published":{"date-parts":[[2023,9,14]]}}}