{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T06:33:34Z","timestamp":1782282814917,"version":"3.54.5"},"reference-count":9,"publisher":"Oxford University Press (OUP)","issue":"16-18","license":[{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"JSPS KAKENHI","award":["JP23K07127"],"award-info":[{"award-number":["JP23K07127"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,11,13]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>As compared to peripheral blood mononuclear cell (PBMC) culture, a lower mitotic index (MI) is seen in whole blood (WB) culture, but WB can be directly used for culture in dicentric chromosome assay (DCA). The purpose of this study is to develop a simple protocol for metaphase enrichment to improve the metaphase frequency of WB culture. Fixed cells were obtained after performing WB and PBMC cultures for DCA after conventional fixation. An additional low-speed centrifugation of 200\u00a0\u00d7\u00a0g for 1\u00a0min was performed, separating the fixed cells of WB culture into a pellet and a supernatant fraction. The additional low-speed centrifugation enriched metaphase frequency and provided an MI comparable to the PBMC culture in the pellet fraction. Our study suggests that it is possible to increase the number of metaphase cells on slides using the slow centrifugation method, which could contribute to the efficiency of chromosome aberration analysis in biodosimetry.<\/jats:p>","DOI":"10.1093\/rpd\/ncae048","type":"journal-article","created":{"date-parts":[[2024,11,14]],"date-time":"2024-11-14T11:31:06Z","timestamp":1731583866000},"page":"1641-1646","source":"Crossref","is-referenced-by-count":2,"title":["An efficient and simple method for enriching metaphase cells for dicentric chromosome assay"],"prefix":"10.1093","volume":"200","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-3519-8911","authenticated-orcid":false,"given":"Ryo","family":"Nakayama","sequence":"first","affiliation":[{"name":"Department of Risk Analysis and Biodosimetry, Institute of Radiation Emergency Medicine, Hirosaki University , 66-1 Hon-cho, Hirosaki 036-8564,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thanh-Mai","family":"Tran","sequence":"additional","affiliation":[{"name":"Department of Risk Analysis and Biodosimetry, Institute of Radiation Emergency Medicine, Hirosaki University , 66-1 Hon-cho, Hirosaki 036-8564,","place":["Japan"]},{"name":"Centre for Radiation Technology and Biological Technology, Dalat Nuclear Research Institute , 1 Nguyen Tu Luc, Ward 8, Dalat city, Lamdong Province 67000,","place":["Vietnam"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6716-4059","authenticated-orcid":false,"given":"Donovan","family":"Anderson","sequence":"additional","affiliation":[{"name":"Department of Risk Analysis and Biodosimetry, Institute of Radiation Emergency Medicine, Hirosaki University , 66-1 Hon-cho, Hirosaki 036-8564,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Takebayashi","sequence":"additional","affiliation":[{"name":"Department of Risk Analysis and Biodosimetry, Institute of Radiation Emergency Medicine, Hirosaki University , 66-1 Hon-cho, Hirosaki 036-8564,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0016-609X","authenticated-orcid":false,"given":"Valerie Swee Ting","family":"Goh","sequence":"additional","affiliation":[{"name":"Department of Radiobiology , Singapore Nuclear Research and Safety Initiative, , 1 Create Way, Singapore 138602,","place":["Singapore"]},{"name":"National University of Singapore , Singapore Nuclear Research and Safety Initiative, , 1 Create Way, Singapore 138602,","place":["Singapore"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5053-3276","authenticated-orcid":false,"given":"Yohei","family":"Fujishima","sequence":"additional","affiliation":[{"name":"Department of Risk Analysis and Biodosimetry, Institute of Radiation Emergency Medicine, Hirosaki University , 66-1 Hon-cho, Hirosaki 036-8564,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3104-2134","authenticated-orcid":false,"given":"Tomisato","family":"Miura","sequence":"additional","affiliation":[{"name":"Department of Risk Analysis and Biodosimetry, Institute of Radiation Emergency Medicine, Hirosaki University , 66-1 Hon-cho, Hirosaki 036-8564,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,11,14]]},"reference":[{"key":"2024111411305585600_ref1","volume-title":"Cytogenetic Dosimetry: Applications in Preparedness for and Response to Radiation Emergencies","author":"International Atomic Energy Agency","year":"2011"},{"issue":"1","key":"2024111411305585600_ref2","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1667\/RR1549.1","article-title":"WHO 1st consultation on the development of a global biodosimetry laboratories network for radiation emergencies (BioDoseNet)","volume":"171","author":"Blakely","year":"2009","journal-title":"Radiat. 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