{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T01:20:46Z","timestamp":1790299246495,"version":"4.1.0"},"reference-count":41,"publisher":"Oxford University Press (OUP)","issue":"7","funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01NS075184"],"award-info":[{"award-number":["R01NS075184"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01NS101283"],"award-info":[{"award-number":["R01NS101283"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P41 EB015909"],"award-info":[{"award-number":["P41 EB015909"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,7,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Study Objectives<\/jats:title><jats:p>Determine abnormalities in levels of iron-management proteins in neuronal origin-enriched extracellular vesicles (nEVs) in restless legs syndrome (RLS).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We used immunoprecipitation for neuronal marker L1CAM to isolate nEVs from the serum of 20 participants with RLS from a study including magnetic resonance imaging (MRI) determinations of iron deposition in the substantia nigra and hematologic parameters and 28 age- and sex-matched Controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>RLS compared with Control participants showed higher levels of nEV total ferritin but similar levels of transferrin receptor and ferroportin. Western blot analysis showed that heavy- but not light-chain ferritin was increased in nEVs of RLS compared with Control participants. In RLS but not Control participants, nEV total ferritin was positively correlated with systemic iron parameters; the two groups also differed in the relation of nEV total ferritin to MRI measures of iron deposition in substantia nigra.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Given the neuronal origin and diversity of EV cargo, nEVs provide an important platform for exploring the underlying pathophysiology and possible biomarkers of RLS.<\/jats:p><\/jats:sec>","DOI":"10.1093\/sleep\/zsz079","type":"journal-article","created":{"date-parts":[[2019,3,21]],"date-time":"2019-03-21T05:40:00Z","timestamp":1553146800000},"source":"Crossref","is-referenced-by-count":24,"title":["Extracellular vesicles reveal abnormalities in neuronal iron metabolism in restless legs syndrome"],"prefix":"10.1093","volume":"42","author":[{"given":"Sahil","family":"Chawla","sequence":"first","affiliation":[{"name":"Laboratory of Neurosciences, Intramural Research Program, National Institute on Aging, NIH, Baltimore, MD"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seema","family":"Gulyani","sequence":"additional","affiliation":[{"name":"Johns Hopkins Sleep Disorders Center, Johns Hopkins Bayview Medical Center, Baltimore, MD"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard P","family":"Allen","sequence":"additional","affiliation":[{"name":"Department of Neurology, The Johns Hopkins Center for Restless Legs Syndrome, Johns Hopkins Bayview Medical Center, Baltimore, MD"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher J","family":"Earley","sequence":"additional","affiliation":[{"name":"Department of Neurology, The Johns Hopkins Center for Restless Legs Syndrome, Johns Hopkins Bayview Medical Center, Baltimore, MD"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Radiology and Radiological Science, Division of MR Research, Johns Hopkins Medicine, Baltimore, MD"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Van Zijl","sequence":"additional","affiliation":[{"name":"Department of Radiology and Radiological Science, Division of MR Research, Johns Hopkins Medicine, Baltimore, MD"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2181-3118","authenticated-orcid":false,"given":"Dimitrios","family":"Kapogiannis","sequence":"additional","affiliation":[{"name":"Laboratory of Neurosciences, Intramural Research Program, National Institute on Aging, NIH, Baltimore, MD"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2019,3,21]]},"reference":[{"issue":"21","key":"2019070802254074300_CIT0001","doi-asserted-by":"crossref","first-page":"2103","DOI":"10.1056\/NEJMcp021288","article-title":"Clinical practice. 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