{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T16:23:17Z","timestamp":1761582197280,"version":"3.37.3"},"reference-count":40,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2022,6,8]],"date-time":"2022-06-08T00:00:00Z","timestamp":1654646400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Division of Thoracic Surgery of the University Hospital of Modena"},{"name":"Laboratory of Cellular Therapy of the University of Modena and Reggio Emilia"},{"name":"Myriad Inc."},{"name":"Italian Ministry of Education, University and Research: Departments of Excellence 2017"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,8,23]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>Cancer stem cells (CSCs) are described as resistant to chemotherapy and radiotherapy. It has been shown that CSCs influence disease-free survival in patients undergoing surgery for lung cancer (NCT04634630). We recently described an overexpression of CSCs recurrence-related genes (RG) in lung cancer. This study aims to investigate CSC frequency and RG expression as predictors of disease-free survival in lung cancer.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental Design<\/jats:title><jats:p>This secondary analysis of a prospective cohort study involved 22 surgical tumor specimens from 22 patients harboring early (I-II) and locally advanced (IIIA) stages ACL and SCCL. Cell population frequency analysis of ALDHhigh (CSCs) and ALDHlow (cancer cells) was performed on each tumor specimen. In addition, RG expression was assessed for 31 target genes separately in ALDHhigh and ALDHlow populations. CSCs frequency and RG expression were assessed as predictors of disease-free survival by Cox analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>CSCs frequency and RG expression were independent predictors of disease-free survival. CSC frequency was not related to disease-free survival in early-stage patients (HR = 0.84, 95%CI = 0.53-1.33, P = .454), whereas it was a risk factor for locally advanced-stage patients (HR = 1.22, 95%CI = 1.09-1.35, P = .000). RG expression\u2014if measured in CSCs\u2014was related to a higher risk of recurrence (HR = 1.19, 95%CI = 1.03-1.39, P = .021). The effect of RG expression measured in cancer cells on disease-free survival was lower and was not statistically significant (HR = 1.12, 95%CI = 0.94-1.33, P = .196).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>CSCs frequency and RG expression are independent predictors of relapse in lung cancer. Considering these results, CSCs and RG may be considered for both target therapy and prognosis.<\/jats:p><\/jats:sec>","DOI":"10.1093\/stcltm\/szac040","type":"journal-article","created":{"date-parts":[[2022,6,8]],"date-time":"2022-06-08T12:20:36Z","timestamp":1654690836000},"page":"797-804","source":"Crossref","is-referenced-by-count":4,"title":["Cancer Stem Cells and Cell Cycle Genes as Independent Predictors of Relapse in Non-small Cell Lung Cancer: Secondary Analysis of a Prospective Study"],"prefix":"10.1093","volume":"11","author":[{"given":"Valentina","family":"Masciale","sequence":"first","affiliation":[{"name":"Division of Thoracic Surgery, Department of Medical and Surgical Sciences, University of Modena and Reggio Emilia , Modena , Italy"},{"name":"Division of Oncology, Department of Medical and Surgical Sciences, University of Modena and Reggio Emilia , Modena , Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Federico","family":"Banchelli","sequence":"additional","affiliation":[{"name":"Center of Medical Statistics, Department of Medical and Surgical Sciences, University of Modena and Reggio Emilia , Modena , Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giulia","family":"Grisendi","sequence":"additional","affiliation":[{"name":"Division of Oncology, Department of Medical and Surgical Sciences, University of Modena and Reggio Emilia , Modena , Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto","family":"D\u2019Amico","sequence":"additional","affiliation":[{"name":"Center of Medical Statistics, Department of Medical and Surgical Sciences, University of Modena and Reggio Emilia , Modena , Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9634-2520","authenticated-orcid":false,"given":"Antonino","family":"Maiorana","sequence":"additional","affiliation":[{"name":"Institute of Pathology, Department of Medical and Surgical Sciences, University of Modena and Reggio Emilia , Modena , Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandro","family":"Stefani","sequence":"additional","affiliation":[{"name":"Division of Thoracic Surgery, Department of Medical and Surgical Sciences, University of Modena and Reggio Emilia , Modena , Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Uliano","family":"Morandi","sequence":"additional","affiliation":[{"name":"Division of Thoracic Surgery, Department of Medical and Surgical Sciences, University of Modena and Reggio Emilia , Modena , Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Franco","family":"Stella","sequence":"additional","affiliation":[{"name":"Division of Thoracic Surgery, Department of Experimental, Diagnostic and Specialty Medicine\u2014DIMES of the Alma Mater Studiorum, University of Bologna, G.B. 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