{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T18:03:48Z","timestamp":1793124228752,"version":"4.5.0"},"reference-count":66,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2022,9,14]],"date-time":"2022-09-14T00:00:00Z","timestamp":1663113600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["175300"],"award-info":[{"award-number":["175300"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["176535"],"award-info":[{"award-number":["176535"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001465","name":"American Thoracic Society","doi-asserted-by":"publisher","award":["RP-2020-26"],"award-info":[{"award-number":["RP-2020-26"]}],"id":[{"id":"10.13039\/100001465","id-type":"DOI","asserted-by":"publisher"}]},{"name":"SickKids Restracomp Fellowship"},{"name":"SickKids Foundation","award":["R00DH00000"],"award-info":[{"award-number":["R00DH00000"]}]},{"name":"University of Toronto Department of Surgery"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,10,21]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Pulmonary hypoplasia secondary to congenital diaphragmatic hernia (CDH) is characterized by impaired branching morphogenesis and differentiation. We have previously demonstrated that administration of extracellular vesicles derived from rat amniotic fluid stem cells (AFSC-EVs) rescues development of hypoplastic lungs at the pseudoglandular and alveolar stages in rodent models of CDH. Herein, we tested whether AFSC-EVs exert their regenerative effects at the canalicular and saccular stages, as these are translationally relevant for clinical intervention. To induce fetal pulmonary hypoplasia, we gavaged rat dams with nitrofen at embryonic day 9.5 and demonstrated that nitrofen-exposed lungs had impaired branching morphogenesis, dysregulated signaling pathways relevant to lung development (FGF10\/FGFR2, ROBO\/SLIT, Ephrin, Neuropilin 1, \u03b2-catenin) and impaired epithelial and mesenchymal cell marker expression at both stages. AFSC-EVs administered to nitrofen-exposed lung explants rescued airspace density and increased the expression levels of key factors responsible for branching morphogenesis. Moreover, AFSC-EVs rescued the expression of alveolar type 1 and 2 cell markers at both canalicular and saccular stages and restored markers of club, ciliated epithelial, and pulmonary neuroendocrine cells at the saccular stage. AFSC-EV-treated lungs also had restored markers of lipofibroblasts and PDGFRA+ cells to control levels at both stages. EV tracking showed uptake of AFSC-EV RNA cargo throughout the fetal lung and an mRNA-miRNA network analysis identified that several miRNAs responsible for regulating lung development processes were contained in the AFSC-EV cargo. These findings suggest that AFSC-EV-based therapies hold potential for restoring fetal lung growth and maturation in babies with pulmonary hypoplasia secondary to CDH.<\/jats:p>","DOI":"10.1093\/stcltm\/szac063","type":"journal-article","created":{"date-parts":[[2022,9,14]],"date-time":"2022-09-14T17:10:37Z","timestamp":1663175437000},"page":"1089-1102","source":"Crossref","is-referenced-by-count":35,"title":["Treatment with Amniotic Fluid Stem Cell Extracellular Vesicles Promotes Fetal Lung Branching and Cell Differentiation at Canalicular and Saccular Stages in Experimental Pulmonary Hypoplasia Secondary to Congenital Diaphragmatic Hernia"],"prefix":"10.1093","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5947-6500","authenticated-orcid":false,"given":"Kasra","family":"Khalaj","sequence":"first","affiliation":[{"name":"Developmental and Stem Cell Biology Program, Peter Gilgan Centre for Research and Learning, The Hospital for Sick Children , Toronto, ON , Canada"},{"name":"Division of General and Thoracic Surgery, The Hospital for Sick Children , Toronto, ON , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7102-1322","authenticated-orcid":false,"given":"Rebeca Lopes","family":"Figueira","sequence":"additional","affiliation":[{"name":"Developmental and Stem Cell Biology Program, Peter Gilgan Centre for Research and Learning, The Hospital for Sick Children , Toronto, ON , Canada"},{"name":"Division of General and Thoracic Surgery, The Hospital for Sick Children , Toronto, ON , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8307-3306","authenticated-orcid":false,"given":"Lina","family":"Antounians","sequence":"additional","affiliation":[{"name":"Developmental and Stem Cell Biology Program, Peter Gilgan Centre for Research and Learning, The Hospital for Sick Children , Toronto, ON , Canada"},{"name":"Division of General and Thoracic Surgery, The Hospital for Sick Children , Toronto, ON , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sree","family":"Gandhi","sequence":"additional","affiliation":[{"name":"Developmental and Stem Cell Biology Program, Peter Gilgan Centre for Research and Learning, The Hospital for Sick Children , Toronto, ON , Canada"},{"name":"Division of General and Thoracic Surgery, The Hospital for Sick Children , Toronto, ON , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Wales","sequence":"additional","affiliation":[{"name":"Developmental and Stem Cell Biology Program, Peter Gilgan Centre for Research and Learning, The Hospital for Sick Children , Toronto, ON , Canada"},{"name":"Division of General and Thoracic Surgery, The Hospital for Sick Children , Toronto, ON , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8357-9609","authenticated-orcid":false,"given":"Louise","family":"Montalva","sequence":"additional","affiliation":[{"name":"Developmental and Stem Cell Biology Program, Peter Gilgan Centre for Research and Learning, The Hospital for Sick Children , Toronto, ON , Canada"},{"name":"Division of General and Thoracic Surgery, The Hospital for Sick Children , Toronto, ON , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George","family":"Biouss","sequence":"additional","affiliation":[{"name":"Developmental and Stem Cell Biology Program, Peter Gilgan Centre for Research and Learning, The Hospital for Sick Children , Toronto, ON , Canada"},{"name":"Division of General and Thoracic Surgery, The Hospital for Sick Children , Toronto, ON , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2283-9846","authenticated-orcid":false,"given":"Augusto","family":"Zani","sequence":"additional","affiliation":[{"name":"Developmental and Stem Cell Biology Program, Peter Gilgan Centre for Research and Learning, The Hospital for Sick Children , Toronto, ON , Canada"},{"name":"Division of General and Thoracic Surgery, The Hospital for Sick Children , Toronto, ON , Canada"},{"name":"Department of Surgery, University of Toronto , Toronto, ON , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,9,14]]},"reference":[{"key":"2022102113291862700_CIT0001","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1016\/j.siny.2017.06.004","article-title":"Pulmonary hypoplasia","volume":"22","author":"Cotten","year":"2017","journal-title":"Semin Fetal Neonatal Med"},{"key":"2022102113291862700_CIT0002","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1038\/s41572-022-00362-w","article-title":"Congenital diaphragmatic hernia","volume":"8","author":"Zani","year":"2022","journal-title":"Nat Rev Dis Primers"},{"key":"2022102113291862700_CIT0003","doi-asserted-by":"crossref","DOI":"10.1097\/SLA.0000000000005113","article-title":"Mortality in congenital diaphragmatic hernia: a multicenter registry study of over 5000 patients over 25 years","author":"Gupta","year":"2021","journal-title":"Ann Surg"},{"key":"2022102113291862700_CIT0004","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1002\/ppul.22557","article-title":"Lung function in young adults with congenital diaphragmatic hernia; a longitudinal evaluation","volume":"48","author":"Spoel","year":"2013","journal-title":"Pediatr Pulmonol"},{"key":"2022102113291862700_CIT0005","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1136\/thx.31.4.450","article-title":"Persistent hypoplasia of the lung after repair of congenital diaphragmatic hernia","volume":"31","author":"Hislop","year":"1976","journal-title":"Thorax"},{"key":"2022102113291862700_CIT0006","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/j.siny.2014.09.007","article-title":"Novel non-surgical prenatal approaches to treating congenital diaphragmatic hernia","volume":"19","author":"Jeanty","year":"2014","journal-title":"Semin Fetal Neonatal Med"},{"key":"2022102113291862700_CIT0007","doi-asserted-by":"crossref","first-page":"eaax5941","DOI":"10.1126\/scitranslmed.aax5941","article-title":"Fetal lung underdevelopment is rescued by administration of amniotic fluid stem cell extracellular vesicles in rodents","volume":"13","author":"Antounians","year":"2021","journal-title":"Sci Transl Med"},{"key":"2022102113291862700_CIT0008","doi-asserted-by":"crossref","first-page":"784","DOI":"10.1161\/CIRCRESAHA.108.181818","article-title":"Branching morphogenesis","volume":"103","author":"Horowitz","year":"2008","journal-title":"Circ Res"},{"key":"2022102113291862700_CIT0009","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1242\/dev.098186","article-title":"Lung development: orchestrating the generation and regeneration of a complex organ","volume":"141","author":"Herriges","year":"2014","journal-title":"Development"},{"key":"2022102113291862700_CIT0010","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1038\/s41467-018-07770-1","article-title":"Single cell RNA analysis identifies cellular heterogeneity and adaptive responses of the lung at birth","volume":"10","author":"Guo","year":"2019","journal-title":"Nat Commun"},{"key":"2022102113291862700_CIT0011","doi-asserted-by":"crossref","first-page":"L733","DOI":"10.1152\/ajplung.00139.2017","article-title":"LungMAP: the molecular atlas of lung development program","volume":"313","author":"Ardini-Poleske","year":"2017","journal-title":"Am J Physiol Lung Cell Mol Physiol"},{"key":"2022102113291862700_CIT0012","doi-asserted-by":"crossref","first-page":"1837","DOI":"10.1038\/s41598-018-38320-w","article-title":"The regenerative potential of amniotic fluid stem cell extracellular vesicles: lessons learned by comparing different isolation techniques","volume":"9","author":"Antounians","year":"2019","journal-title":"Sci Rep"},{"key":"2022102113291862700_CIT0013","doi-asserted-by":"crossref","first-page":"1535750","DOI":"10.1080\/20013078.2018.1535750","article-title":"Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines","volume":"7","author":"Thery","year":"2018","journal-title":"J Extracell Vesicles"},{"key":"2022102113291862700_CIT0014","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1055\/s-0039-1693993","article-title":"Congenital diaphragmatic hernia: state of the art in translating experimental research to the bedside","volume":"29","author":"Antounians","year":"2019","journal-title":"Eur J Pediatr Surg"},{"key":"2022102113291862700_CIT0015","doi-asserted-by":"crossref","first-page":"L1027","DOI":"10.1152\/ajplung.00435.2005","article-title":"Gene expression profiling identifies regulatory pathways involved in the late stage of rat fetal lung development","volume":"291","author":"Weng","year":"2006","journal-title":"Am J Physiol Lung Cell Mol Physiol"},{"key":"2022102113291862700_CIT0016","first-page":"L424","article-title":"Spatial and temporal differences in fibroblast behavior in fetal rat lung","volume":"261","author":"Caniggia","year":"1991","journal-title":"Am J Physiol"},{"key":"2022102113291862700_CIT0017","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1007\/s00383-018-4376-4","article-title":"Endoplasmic reticulum stress response is activated in pulmonary hypoplasia secondary to congenital diaphragmatic hernia, but is decreased by administration of amniotic fluid stem cells","volume":"35","author":"Tzanetakis","year":"2019","journal-title":"Pediatr Surg Int"},{"key":"2022102113291862700_CIT0018","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1164\/rccm.200809-1522ST","article-title":"An official research policy statement of the American Thoracic Society\/European Respiratory Society: standards for quantitative assessment of lung structure","volume":"181","author":"Hsia","year":"2010","journal-title":"Am J Respir Crit Care Med"},{"key":"2022102113291862700_CIT0019","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1373\/clinchem.2008.112797","article-title":"The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments","volume":"55","author":"Bustin","year":"2009","journal-title":"Clin Chem"},{"key":"2022102113291862700_CIT0020","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.ajpath.2011.09.032","article-title":"The pulmonary mesenchymal tissue layer is defective in an in vitro recombinant model of nitrofen-induced lung hypoplasia","volume":"180","author":"van Loenhout","year":"2012","journal-title":"Am J Pathol"},{"key":"2022102113291862700_CIT0021","doi-asserted-by":"crossref","first-page":"2532","DOI":"10.1016\/j.ajpath.2016.07.006","article-title":"Polygenic causes of congenital diaphragmatic hernia produce common lung pathologies","volume":"186","author":"Donahoe","year":"2016","journal-title":"Am J Pathol"},{"key":"2022102113291862700_CIT0022","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1007\/s00383-013-3444-z","article-title":"Disruption of THY-1 signaling in alveolar lipofibroblasts in experimentally induced congenital diaphragmatic hernia","volume":"30","author":"Friedmacher","year":"2014","journal-title":"Pediatr Surg Int"},{"key":"2022102113291862700_CIT0023","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.1007\/s00383-014-3549-z","article-title":"Evidence for decreased lipofibroblast expression in hypoplastic rat lungs with congenital diaphragmatic hernia","volume":"30","author":"Friedmacher","year":"2014","journal-title":"Pediatr Surg Int"},{"key":"2022102113291862700_CIT0024","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1016\/j.jpedsurg.2012.01.038","article-title":"Alterations of peroxisome proliferator-activated receptor \u03b3 and monocyte chemoattractant protein 1 gene expression in the nitrofen-induced hypoplastic lung","volume":"47","author":"Gosemann","year":"2012","journal-title":"J Pediatr Surg"},{"key":"2022102113291862700_CIT0025","doi-asserted-by":"crossref","first-page":"876","DOI":"10.1016\/j.jpedsurg.2014.01.017","article-title":"Prenatal retinoic acid increases lipofibroblast expression in hypoplastic rat lungs with experimental congenital diaphragmatic hernia","volume":"49","author":"Friedmacher","year":"2014","journal-title":"J Pediatr Surg"},{"key":"2022102113291862700_CIT0026","doi-asserted-by":"crossref","first-page":"1989","DOI":"10.1016\/j.jpedsurg.2010.06.014","article-title":"Abnormal platelet-derived growth factor signaling accounting for lung hypoplasia in experimental congenital diaphragmatic hernia","volume":"45","author":"Dingemann","year":"2010","journal-title":"J Pediatr Surg"},{"key":"2022102113291862700_CIT0027","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1002\/sctm.20-0199","article-title":"Human induced pluripotent stem cell-derived lung organoids in an ex vivo model of the congenital diaphragmatic hernia fetal lung","volume":"10","author":"Kunisaki","year":"2021","journal-title":"Stem Cells Transl Med"},{"key":"2022102113291862700_CIT0028","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1007\/s00383-018-4375-5","article-title":"Assessment of the nitrofen model of congenital diaphragmatic hernia and of the dysregulated factors involved in pulmonary hypoplasia","volume":"35","author":"Montalva","year":"2019","journal-title":"Pediatr Surg Int"},{"key":"2022102113291862700_CIT0029","doi-asserted-by":"crossref","first-page":"e36865","DOI":"10.7554\/eLife.36865","article-title":"Pdgfra marks a cellular lineage with distinct contributions to myofibroblasts in lung maturation and injury response","volume":"7","author":"Li","year":"2018","journal-title":"eLife"},{"key":"2022102113291862700_CIT0030","doi-asserted-by":"crossref","first-page":"1021","DOI":"10.1016\/S0022-3468(03)00183-0","article-title":"Gene expression of fibroblast growth factors 10 and 7 is downregulated in the lung of nitrofen-induced diaphragmatic hernia in rats.","volume":"38","author":"Teramoto","year":"2003","journal-title":"J Pediatr Surg"},{"key":"2022102113291862700_CIT0031","doi-asserted-by":"crossref","first-page":"L250","DOI":"10.1152\/ajplung.2001.281.1.L250","article-title":"Novel mechanisms in murine nitrofen-induced pulmonary hypoplasia: FGF-10 rescue in culture","volume":"281","author":"Acosta","year":"2001","journal-title":"Am J Physiol Lung Cell Mol Physiol"},{"key":"2022102113291862700_CIT0032","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/j.jpedsurg.2019.10.033","article-title":"Congenital diaphragmatic hernia as a potential target for transamniotic stem cell therapy","volume":"55","author":"Chalphin","year":"2020","journal-title":"J Pediatr Surg"},{"key":"2022102113291862700_CIT0033","doi-asserted-by":"crossref","first-page":"1683","DOI":"10.3727\/096368912X657756","article-title":"Amniotic fluid stem cells rescue both in vitro and in vivo growth, innervation, and motility in nitrofen-exposed hypoplastic rat lungs through paracrine effects","volume":"22","author":"Pederiva","year":"2013","journal-title":"Cell Transplant"},{"key":"2022102113291862700_CIT0034","doi-asserted-by":"crossref","first-page":"L559","DOI":"10.1152\/ajplung.00498.2005","article-title":"Peristalsis of airway smooth muscle is developmentally regulated and uncoupled from hypoplastic lung growth","volume":"291","author":"Jesudason","year":"2006","journal-title":"Am J Physiol Lung Cell Mol Physiol"},{"key":"2022102113291862700_CIT0035","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1007\/s00383-011-2985-2","article-title":"Upregulation of fibroblast growth factor receptor 2 and 3 in the late stages of fetal lung development in the nitrofen rat model","volume":"28","author":"Friedmacher","year":"2012","journal-title":"Pediatr Surg Int"},{"key":"2022102113291862700_CIT0036","doi-asserted-by":"crossref","first-page":"15062","DOI":"10.1073\/pnas.251407098","article-title":"Inadequate lung development and bronchial hyperplasia in mice with a targeted deletion in the Dutt1\/Robo1 gene","volume":"98","author":"Xian","year":"2001","journal-title":"Proc Natl Acad Sci USA"},{"key":"2022102113291862700_CIT0037","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1002\/dvdy.20045","article-title":"Slit and robo expression in the developing mouse lung","volume":"230","author":"Greenberg","year":"2004","journal-title":"Dev Dyn"},{"key":"2022102113291862700_CIT0038","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1186\/s12931-020-01568-w","article-title":"ROBO2 signaling in lung development regulates SOX2\/SOX9 balance, branching morphogenesis and is dysregulated in nitrofen-induced congenital diaphragmatic hernia","volume":"21","author":"Gon\u00e7alves","year":"2020","journal-title":"Respir Res"},{"key":"2022102113291862700_CIT0039","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1055\/s-0038-1675774","article-title":"Ephrin-B1, -B2, and -B4 expression is decreased in developing diaphragms and lungs of fetal rats with nitrofen-induced congenital diaphragmatic hernia","volume":"29","author":"Takahashi","year":"2019","journal-title":"Eur J Pediatr Surg"},{"key":"2022102113291862700_CIT0040","doi-asserted-by":"crossref","first-page":"L179","DOI":"10.1152\/ajplung.00149.2020","article-title":"Neuropilin-1 directs PDGFR\u03b1-entry into lung fibroblasts and signaling from very early endosomes","volume":"320","author":"McGowan","year":"2021","journal-title":"Am J Physiol Lung Cell Mol Physiol"},{"key":"2022102113291862700_CIT0041","doi-asserted-by":"crossref","first-page":"dev160788","DOI":"10.1242\/dev.160788","article-title":"\u03b2-Catenin maintains lung epithelial progenitors after lung specification","volume":"145","author":"Ostrin","year":"2018","journal-title":"Development"},{"key":"2022102113291862700_CIT0042","doi-asserted-by":"crossref","first-page":"e0214793","DOI":"10.1371\/journal.pone.0214793","article-title":"The proportion of alveolar type 1 cells decreases in murine hypoplastic congenital diaphragmatic hernia lungs","volume":"14","author":"Nguyen","year":"2019","journal-title":"PLoS One"},{"key":"2022102113291862700_CIT0043","doi-asserted-by":"crossref","first-page":"L44","DOI":"10.1152\/ajplung.00342.2004","article-title":"Congenital diaphragmatic hernia, tracheal occlusion, thyroid transcription factor-1, and fetal pulmonary epithelial maturation","volume":"289","author":"Chapin","year":"2005","journal-title":"Am J Physiol Lung Cell Mol Physiol"},{"key":"2022102113291862700_CIT0044","doi-asserted-by":"crossref","first-page":"L1159","DOI":"10.1152\/ajplung.2000.279.6.L1159","article-title":"Lung hypoplasia in the nitrofen model of congenital diaphragmatic hernia occurs early in development","volume":"279","author":"Guilbert","year":"2000","journal-title":"Am J Physiol Lung Cell Mol Physiol"},{"key":"2022102113291862700_CIT0045","article-title":"Murine fetal tracheal occlusion increases lung basal cells via increased yap signaling","author":"Serapiglia","year":"2021","journal-title":"MedRxiv"},{"key":"2022102113291862700_CIT0046","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1007\/s003830050549","article-title":"Pulmonary neuroendocrine cells in nitrofen-induced diaphragmatic hernia and the effect of prenatal glucocorticoids","volume":"15","author":"Gosney","year":"1999","journal-title":"Pediatr Surg Int"},{"key":"2022102113291862700_CIT0047","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1016\/j.jpedsurg.2007.02.014","article-title":"Pulmonary epithelial cell differentiation in the nitrofen-induced congenital diaphragmatic hernia","volume":"42","author":"Santos","year":"2007","journal-title":"J Pediatr Surg"},{"key":"2022102113291862700_CIT0048","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/0022-3468(95)90044-6","article-title":"Pulmonary neuroendocrine cells in neonatal rats with congenital diaphragmatic hernia","volume":"30","author":"IJsselstijn","year":"1995","journal-title":"J Pediatr Surg"},{"key":"2022102113291862700_CIT0049","doi-asserted-by":"crossref","first-page":"17531","DOI":"10.1073\/pnas.1207238109","article-title":"Functional characterization of pulmonary neuroendocrine cells in lung development, injury, and tumorigenesis","volume":"109","author":"Song","year":"2012","journal-title":"Proc Natl Acad Sci USA"},{"key":"2022102113291862700_CIT0050","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.matbio.2020.05.002","article-title":"The elephant in the lung: integrating lineage-tracing, molecular markers, and single cell sequencing data to identify distinct fibroblast populations during lung development and regeneration","volume":"91-92","author":"Riccetti","year":"2020","journal-title":"Matrix Biol"},{"key":"2022102113291862700_CIT0051","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/j.stem.2016.10.004","article-title":"Two-way conversion between lipogenic and myogenic fibroblastic phenotypes marks the progression and resolution of lung fibrosis","volume":"20","author":"El Agha","year":"2017","journal-title":"Cell Stem Cell"},{"key":"2022102113291862700_CIT0052","doi-asserted-by":"crossref","first-page":"1920","DOI":"10.1038\/s41467-020-15647-5","article-title":"Collagen-producing lung cell atlas identifies multiple subsets with distinct localization and relevance to fibrosis","volume":"11","author":"Tsukui","year":"2020","journal-title":"Nat Commun"},{"key":"2022102113291862700_CIT0053","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1080\/01902140600880257","article-title":"Evidence for the presence of lipofibroblasts in human lung","volume":"32","author":"Rehan","year":"2006","journal-title":"Exp Lung Res"},{"key":"2022102113291862700_CIT0054","doi-asserted-by":"crossref","first-page":"L231","DOI":"10.1152\/ajplung.00342.2013","article-title":"Reduced platelet-derived growth factor receptor expression is a primary feature of human bronchopulmonary dysplasia","volume":"307","author":"Popova","year":"2014","journal-title":"Am J Physiol Lung Cell Mol Physiol"},{"key":"2022102113291862700_CIT0055","doi-asserted-by":"crossref","first-page":"17070","DOI":"10.1038\/s41598-018-35256-z","article-title":"Gene expression signatures point to a male sex-specific lung mesenchymal cell PDGF receptor signaling defect in infants developing bronchopulmonary dysplasia","volume":"8","author":"Fulton","year":"2018","journal-title":"Sci Rep"},{"key":"2022102113291862700_CIT0056","doi-asserted-by":"crossref","first-page":"dev176354","DOI":"10.1242\/dev.176354","article-title":"Secondary crest myofibroblast PDGFR\u03b1 controls the elastogenesis pathway via a secondary tier of signaling networks during alveologenesis","volume":"146","author":"Li","year":"2019","journal-title":"Development"},{"key":"2022102113291862700_CIT0057","doi-asserted-by":"crossref","first-page":"7664","DOI":"10.1111\/jcmm.14639","article-title":"miR-20a-5p regulates pulmonary surfactant gene expression in alveolar type II cells","volume":"23","author":"Gong","year":"2019","journal-title":"J Cell Mol Med"},{"key":"2022102113291862700_CIT0058","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1164\/rccm.202109-2168OC","article-title":"Autophagy is impaired in fetal hypoplastic lungs and rescued by administration of amniotic fluid stem cell extracellular vesicles","volume":"206","author":"Khalaj","journal-title":"Am J Respir Crit Care Med"},{"key":"2022102113291862700_CIT0059","doi-asserted-by":"crossref","first-page":"1096","DOI":"10.1016\/j.jpedsurg.2016.02.083","article-title":"Decreased expression of miR-33 in fetal lungs of nitrofen-induced congenital diaphragmatic hernia rat model","volume":"51","author":"Zhu","year":"2016","journal-title":"J Pediatr Surg"},{"key":"2022102113291862700_CIT0060","doi-asserted-by":"crossref","first-page":"854","DOI":"10.1038\/ng.905","article-title":"MicroRNAs can generate thresholds in target gene expression","volume":"43","author":"Mukherji","year":"2011","journal-title":"Nat Genet"},{"key":"2022102113291862700_CIT0061","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1056\/NEJMoa2027030","article-title":"Randomized trial of fetal surgery for severe left diaphragmatic hernia","volume":"385","author":"Deprest","year":"2021","journal-title":"N Engl J Med"},{"key":"2022102113291862700_CIT0062","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1056\/NEJMoa2026983","article-title":"Randomized trial of fetal surgery for moderate left diaphragmatic hernia","volume":"385","author":"Deprest","year":"2021","journal-title":"N Engl J Med"},{"key":"2022102113291862700_CIT0063","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1016\/j.jcyt.2018.12.006","article-title":"Manufacturing and characterization of extracellular vesicles from umbilical cord-derived mesenchymal stromal cells for clinical testing","volume":"21","author":"Rohde","year":"2019","journal-title":"Cytotherapy"},{"key":"2022102113291862700_CIT0064","doi-asserted-by":"crossref","first-page":"1795365","DOI":"10.1080\/20013078.2020.1795365","article-title":"Systematic review of extracellular vesicle-based treatments for lung injury: are EVs a potential therapy for COVID-19?","volume":"9","author":"Khalaj","year":"2020","journal-title":"J Extracell Vesicles"},{"key":"2022102113291862700_CIT0065","doi-asserted-by":"crossref","first-page":"L147","DOI":"10.1152\/ajplung.00108.2014","article-title":"Metabolic disturbances of the vitamin A pathway in human diaphragmatic hernia","volume":"308","author":"Coste","year":"2015","journal-title":"Am J Physiol Lung Cell Mol Physiol"},{"key":"2022102113291862700_CIT0066","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.earlhumdev.2017.10.005","article-title":"Lung and liver growth and retinoic acid status in human fetuses with congenital diaphragmatic hernia","volume":"116","author":"Loo","year":"2018","journal-title":"Early Hum Dev"}],"container-title":["Stem Cells Translational Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/stcltm\/article-pdf\/11\/10\/1089\/46593705\/szac063.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/stcltm\/article-pdf\/11\/10\/1089\/46593705\/szac063.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,10,21]],"date-time":"2022-10-21T15:25:16Z","timestamp":1666365916000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/stcltm\/article\/11\/10\/1089\/6698011"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,14]]},"references-count":66,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2022,9,14]]},"published-print":{"date-parts":[[2022,10,21]]}},"URL":"https:\/\/doi.org\/10.1093\/stcltm\/szac063","relation":{},"ISSN":["2157-6564","2157-6580"],"issn-type":[{"value":"2157-6564","type":"print"},{"value":"2157-6580","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2022,10,1]]},"published":{"date-parts":[[2022,9,14]]}}}