{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T01:54:40Z","timestamp":1780624480259,"version":"3.54.1"},"reference-count":68,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2023,11,3]],"date-time":"2023-11-03T00:00:00Z","timestamp":1698969600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82271132"],"award-info":[{"award-number":["82271132"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82201224"],"award-info":[{"award-number":["82201224"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82101128"],"award-info":[{"award-number":["82101128"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Top Military Medical Science and Technology Youth Training","award":["20QNPY027"],"award-info":[{"award-number":["20QNPY027"]}]},{"name":"Beijing Hospitals Authority Youth Program","award":["QML20230109"],"award-info":[{"award-number":["QML20230109"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Cone cell death is a characteristic shared by various retinal degenerative disorders, such as cone-rod dystrophy, Stargardt disease, achromatopsia, and retinitis pigmentosa. This leads to conditions like color blindness and permanently impaired visual acuity. Stem cell therapy focused on photoreceptor replacement holds promise for addressing these conditions. However, identifying surface markers that aid in enriching retinal progenitor cells (RPCs) capable of differentiating into cones remains a complex task. In this study, we employed single-cell RNA sequencing to scrutinize the transcriptome of developing retinas in C57BL\/6J mice. This revealed the distinctive expression of somatostatin receptor 2 (Sstr2), a surface protein, in late-stage RPCs exhibiting the potential for photoreceptor differentiation. In vivo lineage tracing experiments verified that Sstr2+ cells within the late embryonic retina gave rise to cones, amacrine and horizontal cells during the developmental process. Furthermore, Sstr2+ cells that were isolated from the late embryonic mouse retina displayed RPC markers and exhibited the capability to differentiate into cones in vitro. Upon subretinal transplantation into both wild-type and retinal degeneration 10 (rd10) mice, Sstr2+ cells survived and expressed cone-specific markers. This study underscores the ability of Sstr2 to enrich late-stage RPCs primed for cone differentiation to a large extent. It proposes the utility of Sstr2 as a biomarker for RPCs capable of generating cones for transplantation purposes.<\/jats:p>","DOI":"10.1093\/stcltm\/szad073","type":"journal-article","created":{"date-parts":[[2023,11,8]],"date-time":"2023-11-08T03:15:56Z","timestamp":1699413356000},"page":"83-99","source":"Crossref","is-referenced-by-count":4,"title":["Sstr2 Defines the Cone Differentiation-Competent Late-Stage Retinal Progenitor Cells in the Developing Mouse Retina"],"prefix":"10.1093","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-0109-9671","authenticated-orcid":false,"given":"Yihan","family":"Bai","sequence":"first","affiliation":[{"name":"Southwest Hospital\/Southwest Eye Hospital, Third Military Medical University (Army Medical University) , Chongqing , People\u2019s Republic of China"},{"name":"Key Lab of Visual Damage and Regeneration & Restoration of Chongqing , Chongqing , People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han","family":"He","sequence":"additional","affiliation":[{"name":"Southwest Hospital\/Southwest Eye Hospital, Third Military Medical University (Army Medical University) , Chongqing , People\u2019s Republic of China"},{"name":"Key Lab of Visual Damage and Regeneration & Restoration of Chongqing , Chongqing , People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bangqi","family":"Ren","sequence":"additional","affiliation":[{"name":"Southwest Hospital\/Southwest Eye Hospital, Third Military Medical University (Army Medical University) , Chongqing , People\u2019s Republic of China"},{"name":"Key Lab of Visual Damage and Regeneration & Restoration of Chongqing , Chongqing , People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayun","family":"Ren","sequence":"additional","affiliation":[{"name":"Southwest Hospital\/Southwest Eye Hospital, Third Military Medical University (Army Medical University) , Chongqing , People\u2019s Republic of China"},{"name":"Key Lab of Visual Damage and Regeneration & Restoration of Chongqing , Chongqing , People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Zou","sequence":"additional","affiliation":[{"name":"Southwest Hospital\/Southwest Eye Hospital, Third Military Medical University (Army Medical University) , Chongqing , People\u2019s Republic of China"},{"name":"Key Lab of Visual Damage and Regeneration & Restoration of Chongqing , Chongqing , People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Beijing Friendship Hospital, Capital Medical University , Beijing , People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Liu","sequence":"additional","affiliation":[{"name":"Southwest Hospital\/Southwest Eye Hospital, Third Military Medical University (Army Medical University) , Chongqing , People\u2019s Republic of China"},{"name":"Key Lab of Visual Damage and Regeneration & Restoration of Chongqing , Chongqing , People\u2019s Republic of China"},{"name":"Jinfeng Laboratory , Chongqing , China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,11,3]]},"reference":[{"key":"2024011211331193000_CIT0001","first-page":"1","article-title":"Photoreceptor cell replacement in macular degeneration and retinitis pigmentosa: a pluripotent stem cell-based approach","volume-title":"Prog Retin Eye Res","author":"Gagliardi","year":"2019"},{"key":"2024011211331193000_CIT0002","first-page":"151","article-title":"Mechanisms of age-related macular degeneration and therapeutic opportunities","volume-title":"J Pathol","author":"van Lookeren Campagne","year":"2014"},{"key":"2024011211331193000_CIT0003","first-page":"115","article-title":"Cell-based therapy for degenerative retinal disease","volume-title":"Trends Mol Med","author":"Zarbin","year":"2016"},{"key":"2024011211331193000_CIT0004","first-page":"38","article-title":"Stemming retinal regeneration with pluripotent stem cells","volume-title":"Prog Retin Eye Res","author":"Jin","year":"2019"},{"key":"2024011211331193000_CIT0005","first-page":"109022","article-title":"Restoration of visual function in advanced disease after transplantation of purified human pluripotent stem cell-derived cone photoreceptors","volume-title":"Cell Rep","author":"Ribeiro","year":"2021"},{"key":"2024011211331193000_CIT0006","first-page":"e154619","article-title":"Transplanted human cones incorporate into the retina and function in a murine cone degeneration model","volume-title":"J Clin Invest","author":"Gasparini","year":"2022"},{"key":"2024011211331193000_CIT0007","first-page":"209","article-title":"Long-term safety of human retinal progenitor cell transplantation in retinitis pigmentosa patients","volume-title":"Stem Cell Res Ther","author":"Liu","year":"2017"},{"key":"2024011211331193000_CIT0008","first-page":"388","article-title":"Strategies of pluripotent stem cell-based therapy for retinal degeneration: update and challenges","volume-title":"Trends Mol Med","author":"Maeda","year":"2022"},{"key":"2024011211331193000_CIT0009","first-page":"3218","article-title":"Mouse multipotent progenitor 5 cells are located at the interphase between hematopoietic stem and progenitor cells","volume-title":"Blood","author":"Sommerkamp","year":"2021"},{"key":"2024011211331193000_CIT0010","first-page":"5411","article-title":"CD73, a novel cell surface antigen that characterizes retinal photoreceptor precursor cells","volume-title":"Invest Ophthalmol Vis Sci","author":"Koso","year":"2009"},{"key":"2024011211331193000_CIT0011","first-page":"141","article-title":"c-kit marks late retinal progenitor cells and regulates their differentiation in developing mouse retina","volume-title":"Dev Biol","author":"Koso","year":"2007"},{"key":"2024011211331193000_CIT0012","first-page":"2469","article-title":"Transplantation of photoreceptor precursors isolated via a cell surface biomarker panel from embryonic stem cell-derived self-forming retina","volume-title":"Stem Cells","author":"Lakowski","year":"2015"},{"key":"2024011211331193000_CIT0013","first-page":"2169","article-title":"c-Kit(+) cells isolated from human fetal retinas represent a new population of retinal progenitor cells","volume-title":"J Cell Sci","author":"Zhou","year":"2015"},{"key":"2024011211331193000_CIT0014","first-page":"1205","article-title":"Organoid-derived C-Kit(+)\/SSEA4(-) human retinal progenitor cells promote a protective retinal microenvironment during transplantation in rodents","volume-title":"Nat Commun","author":"Zou","year":"2019"},{"key":"2024011211331193000_CIT0015","first-page":"665","article-title":"Characterization and transplantation of CD73-positive photoreceptors isolated from human iPSC-derived retinal organoids","volume-title":"Stem Cell Rep","author":"Gagliardi","year":"2018"},{"key":"2024011211331193000_CIT0016","first-page":"870441","article-title":"Generation of an RCVRN-eGFP reporter hiPSC line by CRISPR\/Cas9 to monitor photoreceptor cell development and facilitate the cell enrichment for transplantation","volume-title":"Front Cell Dev Biol","author":"Guan","year":"2022"},{"key":"2024011211331193000_CIT0017","first-page":"1898","article-title":"Isolation and comparative transcriptome analysis of human fetal and iPSC-derived cone photoreceptor cells","volume-title":"Stem Cell Rep","author":"Welby","year":"2017"},{"key":"2024011211331193000_CIT0018","first-page":"191","article-title":"Grafted c-kit(+)\/SSEA1(-) eye-wall progenitor cells delay retinal degeneration in mice by regulating neural plasticity and forming new graft-to-host synapses","volume-title":"Stem Cell Res Ther","author":"Chen","year":"2016"},{"key":"2024011211331193000_CIT0019","first-page":"e8746","article-title":"Current best practices in single-cell RNA-seq analysis: a tutorial","volume-title":"Mol Syst Biol","author":"Luecken","year":"2019"},{"key":"2024011211331193000_CIT0020","first-page":"1202","article-title":"Highly parallel genome-wide expression profiling of individual cells using nanoliter droplets","volume-title":"Cell","author":"Macosko","year":"2015"},{"key":"2024011211331193000_CIT0021","first-page":"189","article-title":"Dissecting the multicellular ecosystem of metastatic melanoma by single-cell RNA-seq","volume-title":"Science (New York, N.Y.)","author":"Tirosh","year":"2016"},{"key":"2024011211331193000_CIT0022","first-page":"1111","article-title":"Single-cell RNA-Seq analysis of retinal development identifies NFI factors as regulating mitotic exit and late-born cell specification","volume-title":"Neuron","author":"Clark","year":"2019"},{"key":"2024011211331193000_CIT0023","first-page":"38","article-title":"Dimensionality reduction for visualizing single-cell data using UMAP","volume-title":"Nat Biotechnol","author":"Becht","year":"2018"},{"key":"2024011211331193000_CIT0024","first-page":"395","article-title":"Math5 defines the ganglion cell competence state in a subpopulation of retinal progenitor cells exiting the cell cycle","volume-title":"Dev Biol","author":"Brzezinski","year":"2012"},{"key":"2024011211331193000_CIT0025","first-page":"7882","article-title":"Transcription factor Olig2 defines subpopulations of retinal progenitor cells biased toward specific cell fates","volume-title":"Proc Natl Acad Sci U S A","author":"Hafler","year":"2012"},{"key":"2024011211331193000_CIT0026","first-page":"1313","article-title":"Cyclin D1, cancer progression, and opportunities in cancer treatment","volume-title":"J Mol Med (Berlin, Germany)","author":"Qie","year":"2016"},{"key":"2024011211331193000_CIT0027","first-page":"545","article-title":"Loss of ITM2A, a novel tumor suppressor of ovarian cancer through G2\/M cell cycle arrest, is a poor prognostic factor of epithelial ovarian cancer","volume-title":"Gynecol Oncol","author":"Nguyen","year":"2016"},{"key":"2024011211331193000_CIT0028","first-page":"2469","article-title":"Ileal FGF15 contributes to fibrosis-associated hepatocellular carcinoma development","volume-title":"Int J Cancer","author":"Uriarte","year":"2015"},{"key":"2024011211331193000_CIT0029","first-page":"411","article-title":"Regulation of cell proliferation in the retinal pigment epithelium: differential regulation of the death-associated protein like-1 DAPL1 by alternative MITF splice forms","volume-title":"Pigment Cell Melanoma Res","author":"Ma","year":"2018"},{"key":"2024011211331193000_CIT0030","first-page":"4262","article-title":"Hes1 directly controls cell proliferation through the transcriptional repression of p27Kip1","volume-title":"Mol Cell Biol","author":"Murata","year":"2005"},{"key":"2024011211331193000_CIT0031","first-page":"92","article-title":"The DNA replication regulator MCM6: an emerging cancer biomarker and target","volume-title":"Clin Chim Acta Int J Clin Chem","author":"Zeng","year":"2021"},{"key":"2024011211331193000_CIT0032","first-page":"77","article-title":"Toxicological and pathological applications of proliferating cell nuclear antigen (PCNA), a novel endogenous marker for cell proliferation","volume-title":"Crit Rev Toxicol","author":"Dietrich","year":"1993"},{"key":"2024011211331193000_CIT0033","first-page":"8506","article-title":"Identification of natural marine compounds as potential inhibitors of CDK2 using molecular docking and molecular dynamics simulation approach","volume-title":"J Biomol Struct Dyn","author":"Ahmad","year":"2022"},{"key":"2024011211331193000_CIT0034","first-page":"1255","article-title":"Otx2 homeobox gene controls retinal photoreceptor cell fate and pineal gland development","volume-title":"Nat Neurosci","author":"Nishida","year":"2003"},{"key":"2024011211331193000_CIT0035","first-page":"E553","article-title":"Forkhead box N4 (Foxn4) activates Dll4-Notch signaling to suppress photoreceptor cell fates of early retinal progenitors","volume-title":"Proc Natl Acad Sci U S A","author":"Luo","year":"2012"},{"key":"2024011211331193000_CIT0036","first-page":"839","article-title":"Protocadherin 17 regulates presynaptic assembly in topographic corticobasal Ganglia circuits","volume-title":"Neuron","author":"Hoshina","year":"2013"},{"key":"2024011211331193000_CIT0037","first-page":"779","article-title":"Whole transcriptome sequencing identifies key circRNAs, lncRNAs, and miRNAs regulating neurogenesis in developing mouse retina","volume-title":"BMC Genomics","author":"Chen","year":"2021"},{"key":"2024011211331193000_CIT0038","first-page":"408","article-title":"IgSF21 promotes differentiation of inhibitory synapses via binding to neurexin2\u03b1","volume-title":"Nat Commun","author":"Tanabe","year":"2017"},{"key":"2024011211331193000_CIT0039","first-page":"678","article-title":"Integrated microRNA and mRNA sequencing analysis of age-related changes to mouse thymic epithelial cells","volume-title":"IUBMB Life","author":"Jia","year":"2018"},{"key":"2024011211331193000_CIT0040","first-page":"eabe4983","article-title":"Atoh7-independent specification of retinal ganglion cell identity","volume-title":"Sci Adv","author":"Brodie-Kommit","year":"2021"},{"key":"2024011211331193000_CIT0041","first-page":"311","article-title":"Dlx1 and Dlx2 function is necessary for terminal differentiation and survival of late-born retinal ganglion cells in the developing mouse retina","volume-title":"Development (Cambridge, England)","author":"de Melo","year":"2005"},{"key":"2024011211331193000_CIT0042","first-page":"2015","article-title":"Single cell sequencing of induced pluripotent stem cell derived retinal ganglion cells (iPSC-RGC) reveals distinct molecular signatures and RGC subtypes","volume-title":"Genes","author":"Gudiseva","year":"18 2021"},{"key":"2024011211331193000_CIT0043","first-page":"93","article-title":"Galanin promotes neuronal differentiation from neural progenitor cells in vitro and contributes to the generation of new olfactory neurons in the adult mouse brain","volume-title":"Exp Neurol","author":"Cordero-Llana","year":"2014"},{"key":"2024011211331193000_CIT0044","first-page":"140","article-title":"PHLDA1 promotes sevoflurane-induced pyroptosis of neuronal cells in developing rats through TRAF6-mediated activation of Rac1","volume-title":"Neurotoxicology","author":"Shu","year":"2022"},{"key":"2024011211331193000_CIT0045","first-page":"297","article-title":"Targeted deletion of Insm2 in mice result in reduced insulin secretion and glucose intolerance","volume-title":"J Transl Med","author":"Wang","year":"2018"},{"key":"2024011211331193000_CIT0046","first-page":"1961","article-title":"Expression of insulinoma-associated 2 (INSM2) in pancreatic islet cells is regulated by the transcription factors Ngn3 and NeuroD1","volume-title":"Endocrinology","author":"Cai","year":"2011"},{"key":"2024011211331193000_CIT0047","first-page":"5709","article-title":"The evolution of duplicated genes of the Cpi-17\/Phi-1 (ppp1r14) family of protein phosphatase 1 inhibitors in teleosts","volume-title":"Int J Mol Sci","author":"Lang","year":"2020"},{"key":"2024011211331193000_CIT0048","first-page":"10962","article-title":"A potential role for somatostatin signaling in regulating retinal neurogenesis","volume-title":"Sci Rep","author":"Weir","year":"2021"},{"key":"2024011211331193000_CIT0049","first-page":"33","article-title":"Lineage tracing","volume-title":"Cell","author":"Kretzschmar","year":"2012"},{"key":"2024011211331193000_CIT0050","first-page":"531","article-title":"Crx, a novel otx-like homeobox gene, shows photoreceptor-specific expression and regulates photoreceptor differentiation","volume-title":"Cell","author":"Furukawa","year":"1997"},{"key":"2024011211331193000_CIT0051","first-page":"26","article-title":"The subcellular localization of Otx2 is cell-type specific and developmentally regulated in the mouse retina","volume-title":"Brain Res Mol Brain Res","author":"Baas","year":"2000"},{"key":"2024011211331193000_CIT0052","first-page":"94","article-title":"Chemically-induced photoreceptor degeneration and protection in mouse iPSC-derived three-dimensional retinal organoids","volume-title":"Stem Cell Res","author":"Ito","year":"2017"},{"key":"2024011211331193000_CIT0053","first-page":"E99","article-title":"Inducible and irreversible control of gene expression using a single transgene","volume-title":"Nucleic Acids Res","author":"Fuhrmann-Benzakein","year":"2000"},{"key":"2024011211331193000_CIT0054","first-page":"e69113","article-title":"Loss of Notch1 disrupts the barrier repair in the corneal epithelium","volume-title":"PLoS One","author":"Movahedan","year":"2013"},{"key":"2024011211331193000_CIT0055","first-page":"215","article-title":"Toward the generation of rod and cone photoreceptors from mouse, monkey and human embryonic stem cells","volume-title":"Nat Biotechnol","author":"Osakada","year":"2008"},{"key":"2024011211331193000_CIT0056","first-page":"1915","article-title":"Retinoic acid and taurine enhance differentiation of the human bone marrow stem cells into cone photoreceptor cells and retinal ganglion cells","volume-title":"J Cell Biochem","author":"Forouzanfar","year":"2021"},{"key":"2024011211331193000_CIT0057","first-page":"4545","article-title":"Cone and rod photoreceptor transplantation in models of the childhood retinopathy Leber congenital amaurosis using flow-sorted Crx-positive donor cells","volume-title":"Hum Mol Genet","author":"Lakowski","year":"2010"},{"key":"2024011211331193000_CIT0058","first-page":"2370","article-title":"Generation of a rod-specific NRL reporter line in human pluripotent stem cells","volume-title":"Sci Rep","author":"Phillips","year":"2018"},{"key":"2024011211331193000_CIT0059","first-page":"1062","article-title":"Hemopoietic stem cells with higher hemopoietic potential reside at the bone marrow endosteum","volume-title":"Stem Cells","author":"Haylock","year":"2007"},{"key":"2024011211331193000_CIT0060","first-page":"257","article-title":"Integrative single-cell analysis","volume-title":"Nat Rev Genet","author":"Stuart","year":"2019"},{"key":"2024011211331193000_CIT0061","first-page":"109994","article-title":"Gene regulatory networks controlling temporal patterning, neurogenesis, and cell-fate specification in mammalian retina","volume-title":"Cell Rep","author":"Lyu","year":"2021"},{"key":"2024011211331193000_CIT0062","first-page":"3519","article-title":"Ascl1 expression defines a subpopulation of lineage-restricted progenitors in the mammalian retina","volume-title":"Development (Cambridge, England)","author":"Brzezinski","year":"2011"},{"key":"2024011211331193000_CIT0063","first-page":"203","article-title":"Retinal repair by transplantation of photoreceptor precursors","volume-title":"Nature","author":"MacLaren","year":"2006"},{"key":"2024011211331193000_CIT0064","first-page":"99","article-title":"Restoration of vision after transplantation of photoreceptors","volume-title":"Nature","author":"Pearson","year":"2012"},{"key":"2024011211331193000_CIT0065","first-page":"317","article-title":"Developmental patterns of somatostatin-receptors and somatostatin-immunoreactivity during early neurogenesis in the rat","volume-title":"Neuroscience","author":"Maubert","year":"1994"},{"key":"2024011211331193000_CIT0066","first-page":"1805","article-title":"Functional mapping of somatostatin receptors in the retina: a review","volume-title":"Vis Res","author":"Thermos","year":"2003"},{"key":"2024011211331193000_CIT0067","first-page":"e13254","article-title":"Somatostatin signalling promotes the differentiation of rod photoreceptors in human pluripotent stem cell-derived retinal organoid","volume-title":"Cell Prolif","author":"Chen","year":"2022"},{"key":"2024011211331193000_CIT0068","first-page":"3282","article-title":"Wiring the binocular visual pathways","volume-title":"Int J Mol Sci","author":"Murcia-Belmonte","year":"2019"}],"container-title":["Stem Cells Translational Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/stcltm\/article-pdf\/13\/1\/83\/55559009\/szad073.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/stcltm\/article-pdf\/13\/1\/83\/55559009\/szad073.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,12]],"date-time":"2024-01-12T11:35:27Z","timestamp":1705059327000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/stcltm\/article\/13\/1\/83\/7343196"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11,3]]},"references-count":68,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2023,11,3]]},"published-print":{"date-parts":[[2024,1,12]]}},"URL":"https:\/\/doi.org\/10.1093\/stcltm\/szad073","relation":{},"ISSN":["2157-6564","2157-6580"],"issn-type":[{"value":"2157-6564","type":"print"},{"value":"2157-6580","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2024,1,1]]},"published":{"date-parts":[[2023,11,3]]}}}