{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T01:59:55Z","timestamp":1793411995604,"version":"4.5.0"},"reference-count":45,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2023,12,16]],"date-time":"2023-12-16T00:00:00Z","timestamp":1702684800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"National University Hospital Child Cancer","award":["23B-001-0500"],"award-info":[{"award-number":["23B-001-0500"]}]},{"DOI":"10.13039\/501100001321","name":"National Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001321","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Korean Ministry of Science","award":["NRF-2021M3E5D9021368"],"award-info":[{"award-number":["NRF-2021M3E5D9021368"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,3,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Fetal spinal cord ischemia is a serious medical condition that can result in significant neurological damage and adverse outcomes for the fetus. However, the lack of an appropriate experimental model has hindered the understanding of the pathology and the development of effective treatments. In our study, we established a system for screening drugs that affect fetal spinal cord ischemia using spinal cord organoids. Importantly, we produced necrotic core-free human spinal cord organoids (nf-hSCOs) by reducing the organoid size to avoid potential complications of spontaneous necrosis in large organoids. Exposing nf-hSCOs to CoCl2 as a hypoxia mimetic and hypoglycemic conditions resulted in significant neuronal damage, as assessed by multiple assay batteries. By utilizing this model, we tested chemicals that have been reported to exhibit beneficial effects in brain organoid-based ischemia models. Surprisingly, these chemicals did not provide sufficient benefit, and we discovered that rapamycin is a mild neuroprotective reagent for both axon degeneration and neuronal survival. We propose that nf-hSCO is suitable for large-scale screening of fetal neural ischemia due to its scalability, ease of ischemic induction, implementation of quantifiable assay batteries, and the absence of spontaneous necrosis.<\/jats:p>","DOI":"10.1093\/stcltm\/szad089","type":"journal-article","created":{"date-parts":[[2023,12,16]],"date-time":"2023-12-16T16:29:40Z","timestamp":1702744180000},"page":"268-277","source":"Crossref","is-referenced-by-count":18,"title":["Establishment and Validation of a Model for Fetal Neural Ischemia Using Necrotic Core-Free Human Spinal Cord Organoids"],"prefix":"10.1093","volume":"13","author":[{"given":"Aeri","family":"Shin","sequence":"first","affiliation":[{"name":"Department of Anatomy, Korea University College of Medicine , Seoul , Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae Ryun","family":"Ryu","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Korea University College of Medicine , Seoul , Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Byung Gon","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Brain Science, A-Jou University School of Medicine , Suwon , Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1792-4894","authenticated-orcid":false,"given":"Woong","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Korea University College of Medicine , Seoul , Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,12,16]]},"reference":[{"key":"2024031506311903400_CIT0001","first-page":"579","article-title":"Fetal hypoxia insults and patterns of brain injury: insights from animal models","volume-title":"Clin Perinatol","author":"Gunn","year":"2009"},{"key":"2024031506311903400_CIT0002","first-page":"145","article-title":"Fetal stress and 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