{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T05:03:52Z","timestamp":1784351032194,"version":"3.55.0"},"reference-count":49,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2024,2,16]],"date-time":"2024-02-16T00:00:00Z","timestamp":1708041600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science, and Technology of Japan","doi-asserted-by":"crossref","award":["21H0305530"],"award-info":[{"award-number":["21H0305530"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science, and Technology of Japan","doi-asserted-by":"crossref","award":["23K19746"],"award-info":[{"award-number":["23K19746"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,4,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Intravenous administration of conditioned medium from stem cells of human exfoliated deciduous teeth (SHED-CM) regenerates mechanically injured osteochondral tissues in mouse temporomandibular joint osteoarthritis (TMJOA). However, the underlying therapeutic mechanisms remain unclear. Here, we showed that SHED-CM alleviated injured TMJ by inducing anti-inflammatory M2 macrophages in the synovium. Depletion of M2 by Mannosylated Clodrosome abolished the osteochondral repair activities of SHED-CM. Administration of CM from M2-induced by SHED-CM (M2-CM) effectively ameliorated mouse TMJOA by inhibiting chondrocyte inflammation and matrix degradation while enhancing chondrocyte proliferation and matrix formation. Notably, in vitro, M2-CM directly suppressed the catabolic activities while enhancing the anabolic activities of interleukin-1\u03b2-stimulated mouse primary chondrocytes. M2-CM also inhibited receptor activator of nuclear factor NF-\u03baB ligand-induced osteoclastogenesis in RAW264.7 cells. Secretome analysis of M2-CM and M0-CM revealed that 5 proteins related to anti-inflammation and\/or osteochondrogenesis were enriched in M2-CM. Of these proteins, the Wnt signal antagonist, secreted frizzled-related protein 1 (sFRP1), was the most abundant and played an essential role in the shift to anabolic chondrocytes, suggesting that M2 ameliorated TMJOA partly through sFRP1. This study suggests that secretome from SHED exerted remarkable osteochondral regeneration activities in TMJOA through the induction of sFRP1-expressing tissue-repair M2 macrophages.<\/jats:p>","DOI":"10.1093\/stcltm\/szae006","type":"journal-article","created":{"date-parts":[[2024,2,17]],"date-time":"2024-02-17T10:33:40Z","timestamp":1708166020000},"page":"399-413","source":"Crossref","is-referenced-by-count":20,"title":["Conditioned Medium From Stem Cells of Human Exfoliated Deciduous Teeth Alleviates Mouse Osteoarthritis by Inducing sFRP1-Expressing M2 Macrophages"],"prefix":"10.1093","volume":"13","author":[{"given":"Linze","family":"Xia","sequence":"first","affiliation":[{"name":"Department of Tissue Regeneration, Institute of Biomedical Sciences, Tokushima University Graduate School , Tokushima , Japan"},{"name":"Department of Orthodontics and Dentofacial Orthopedics, Institute of Biomedical Sciences, Tokushima University Graduate School , Tokushima , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fumiya","family":"Kano","sequence":"additional","affiliation":[{"name":"Department of Tissue Regeneration, Institute of Biomedical Sciences, Tokushima University Graduate School , Tokushima , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noboru","family":"Hashimoto","sequence":"additional","affiliation":[{"name":"Department of Tissue Regeneration, Institute of Biomedical Sciences, Tokushima University Graduate School , Tokushima , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao","family":"Liu","sequence":"additional","affiliation":[{"name":"Dental School of Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration , Shanghai , People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsendsuren","family":"Khurel-Ochir","sequence":"additional","affiliation":[{"name":"Department of Orthodontics, School of Dentistry, Mongolian National University of Medical Sciences , Ulaanbaatar , Mongolia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoko","family":"Ogasawara","sequence":"additional","affiliation":[{"name":"Department of Orthodontics and Dentofacial Orthopedics, Institute of Biomedical Sciences, Tokushima University Graduate School , Tokushima , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Tissue Regeneration, Institute of Biomedical Sciences, Tokushima University Graduate School , Tokushima , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Tissue Regeneration, Institute of Biomedical Sciences, Tokushima University Graduate School , Tokushima , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideharu","family":"Hibi","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine , Nagoya , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomonori","family":"Iwasaki","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry, Institute of Biomedical Sciences, Tokushima University Graduate School , Tokushima , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eiji","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Department of Orthodontics and Dentofacial Orthopedics, Institute of Biomedical Sciences, Tokushima University Graduate School , Tokushima , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9206-1243","authenticated-orcid":false,"given":"Akihito","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Department of Tissue Regeneration, Institute of Biomedical Sciences, Tokushima University Graduate School , Tokushima , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,2,16]]},"reference":[{"key":"2024041506041003000_CIT0001","first-page":"666","article-title":"Current understanding of pathogenesis and treatment of TMJ osteoarthritis","volume-title":"J Dent Res.","author":"Wang","year":"2015"},{"key":"2024041506041003000_CIT0002","first-page":"8","article-title":"Osteoarthritis of the temporomandibular joint: a narrative overview","volume-title":"Medicina (Kaunas)","author":"M\u00e9lou","year":"2022"},{"key":"2024041506041003000_CIT0003","first-page":"633","article-title":"Initiation and 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