{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T23:04:53Z","timestamp":1793142293112,"version":"4.5.0"},"reference-count":48,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2024,7,11]],"date-time":"2024-07-11T00:00:00Z","timestamp":1720656000000},"content-version":"vor","delay-in-days":1,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,8,16]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Platelet-derived growth factor receptor \u03b1 (PDGFR\u03b1) is often considered as a general marker of mesenchymal cells and fibroblasts, but also shows expression in a portion of osteoprogenitor cells. Within the skeleton, Pdgfr\u03b1+ mesenchymal cells have been identified in bone marrow and periosteum of long bones, where they play a crucial role in participating in fracture repair. A similar examination of Pdgfr\u03b1+ cells in calvarial bone healing has not been examined. Here, we utilize Pdgfr\u03b1-CreERTM;mT\/mG reporter animals to examine the contribution of Pdgfr\u03b1+ mesenchymal cells to calvarial bone repair through histology and single-cell RNA sequencing (scRNA-Seq). Results showed that Pdgfr\u03b1+ mesenchymal cells are present in several cell clusters by scRNA-Seq, and by histology a dramatic increase in Pdgfr\u03b1+ cells populated the defect site at early timepoints to give rise to healed bone tissue overtime. Notably, diphtheria toxin-mediated ablation of Pdgfr\u03b1 reporter+ cells resulted in significantly impaired calvarial bone healing. Our findings suggest that Pdgfr\u03b1-expressing cells within the calvarial niche play a critical role in the process of calvarial bone repair.<\/jats:p>","DOI":"10.1093\/stcltm\/szae041","type":"journal-article","created":{"date-parts":[[2024,7,11]],"date-time":"2024-07-11T00:03:01Z","timestamp":1720656181000},"page":"791-802","source":"Crossref","is-referenced-by-count":4,"title":["Requirement of Pdgfr\u03b1+ cells for calvarial bone repair"],"prefix":"10.1093","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6417-0664","authenticated-orcid":false,"given":"Xin","family":"Xing","sequence":"first","affiliation":[{"name":"Johns Hopkins University Department of Pathology, , Baltimore, MD 21205, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhao","family":"Li","sequence":"additional","affiliation":[{"name":"Johns Hopkins University Department of Pathology, , Baltimore, MD 21205, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiajia","family":"Xu","sequence":"additional","affiliation":[{"name":"Johns Hopkins University Department of Pathology, , Baltimore, MD 21205, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Austin Z","family":"Chen","sequence":"additional","affiliation":[{"name":"Johns Hopkins University Department of Pathology, , Baltimore, MD 21205, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary","family":"Archer","sequence":"additional","affiliation":[{"name":"Johns Hopkins University Department of Pathology, , Baltimore, MD 21205, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiyun","family":"Wang","sequence":"additional","affiliation":[{"name":"Johns Hopkins University Department of Pathology, , Baltimore, MD 21205, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingxin","family":"Xu","sequence":"additional","affiliation":[{"name":"Johns Hopkins University Department of Pathology, , Baltimore, MD 21205, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyi","family":"Wang","sequence":"additional","affiliation":[{"name":"Johns Hopkins University Department of Pathology, , Baltimore, MD 21205, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manyu","family":"Zhu","sequence":"additional","affiliation":[{"name":"Johns Hopkins University Department of Pathology, , Baltimore, MD 21205, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qizhi","family":"Qin","sequence":"additional","affiliation":[{"name":"Johns Hopkins University Department of Pathology, , Baltimore, MD 21205, United 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