{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:34:54Z","timestamp":1787009694267,"version":"build-2736575974"},"reference-count":47,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2025,2,6]],"date-time":"2025-02-06T00:00:00Z","timestamp":1738800000000},"content-version":"vor","delay-in-days":36,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82200690"],"award-info":[{"award-number":["82200690"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32300612"],"award-info":[{"award-number":["32300612"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,1,17]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Cultured red blood cells represent an alternative resource for blood transfusions. However, important issues such as low yields and high costs remain. Recently, gene editing of hematopoietic stem cells has been conducted to induce erythroid differentiation in vitro for producing sufficient RBCs to meet the imbalance in blood supply and demand. The differentiation and expansion of hematopoietic stem and progenitor cells are regulated by transcription factors, such as high mobility group AT-hook 2 (HMGA2). In this study, we utilized CRISPR\/Cas9 to establish a doxycycline-inducible HMGA2-expressing human embryonic stem cell (hESC) line. In a defined erythroid differentiation system, HMGA2 prolonged erythroid differentiation in vitro, enabling extensive expansion of human erythroblasts. The erythroblasts derived from the HMGA2-expressing hESC line are rich in polychromatic and orthochromatic erythroblasts expressing mostly \u03b1- and \u03b3-globin and have the capacity to differentiate into RBCs. Our findings highlight the potential of combining hematopoietic transcription factors with genome editing techniques to enhance RBC production.<\/jats:p>","DOI":"10.1093\/stcltm\/szaf001","type":"journal-article","created":{"date-parts":[[2025,2,6]],"date-time":"2025-02-06T05:24:11Z","timestamp":1738819451000},"source":"Crossref","is-referenced-by-count":2,"title":["3\u2032 UTR-truncated HMGA2 promotes erythroblasts production from human embryonic stem cells"],"prefix":"10.1093","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-2844-4554","authenticated-orcid":false,"given":"Tiantian","family":"Cui","sequence":"first","affiliation":[{"name":"Beijing Institute of Radiation Medicine , Beijing 100850 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoling","family":"Wang","sequence":"additional","affiliation":[{"name":"Beijing Institute of Radiation Medicine , Beijing 100850 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruge","family":"Zang","sequence":"additional","affiliation":[{"name":"Beijing Institute of Radiation Medicine , Beijing 100850 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingping","family":"Zhao","sequence":"additional","affiliation":[{"name":"Beijing Institute of Radiation Medicine , Beijing 100850 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1316-2389","authenticated-orcid":false,"given":"Hao","family":"Yan","sequence":"additional","affiliation":[{"name":"Beijing Institute of Radiation Medicine , Beijing 100850 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuan","family":"Li","sequence":"additional","affiliation":[{"name":"Beijing Institute of Radiation Medicine , Beijing 100850 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenzhao","family":"Xu","sequence":"additional","affiliation":[{"name":"Beijing Institute of Radiation Medicine , Beijing 100850 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiyang","family":"Wang","sequence":"additional","affiliation":[{"name":"Beijing Institute of Radiation Medicine , Beijing 100850 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junnian","family":"Zhou","sequence":"additional","affiliation":[{"name":"Beijing Institute of Radiation Medicine , Beijing 100850 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiming","family":"Liu","sequence":"additional","affiliation":[{"name":"Beijing Institute of Radiation Medicine , Beijing 100850 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Yue","sequence":"additional","affiliation":[{"name":"Beijing Institute of Radiation Medicine , Beijing 100850 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuetao","family":"Pei","sequence":"additional","affiliation":[{"name":"Beijing Institute of Radiation Medicine , Beijing 100850 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-9617-6775","authenticated-orcid":false,"given":"Jiafei","family":"Xi","sequence":"additional","affiliation":[{"name":"Beijing Institute of Radiation Medicine , Beijing 100850 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,2,6]]},"reference":[{"key":"2025040706381752200_CIT0001","doi-asserted-by":"publisher","first-page":"627","DOI":"10.2217\/rme-2018-0025","article-title":"Why industrial production of red blood cells from stem cells is essential for tomorrow\u2019s blood transfusion","volume":"13","author":"Douay","year":"2018","journal-title":"Regen Med"},{"key":"2025040706381752200_CIT0002","doi-asserted-by":"publisher","first-page":"102941","DOI":"10.1016\/j.transci.2020.102941","article-title":"Trends and targets of various types of stem cell derived transfusable RBC substitution therapy: obstacles that need to be converted to opportunity","volume":"59","author":"Lanza","year":"2020","journal-title":"Transfus Apher Sci"},{"key":"2025040706381752200_CIT0003","doi-asserted-by":"publisher","first-page":"108","DOI":"10.1016\/j.transci.2018.12.015","article-title":"Ex vivo generation of transfusable red blood cells from various stem cell sources: a concise revisit of where we are now","volume":"58","author":"Christaki","year":"2019","journal-title":"Transfus Apher Sci"},{"key":"2025040706381752200_CIT0004","doi-asserted-by":"publisher","first-page":"S48","DOI":"10.1002\/sctm.20-0517","article-title":"Therapeutic use of red blood cells and platelets derived from human cord blood stem cells","volume":"10","author":"Xie","year":"2021","journal-title":"Stem Cells Transl. 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