{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T01:13:27Z","timestamp":1790126007539,"version":"4.0.1"},"reference-count":25,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T00:00:00Z","timestamp":1763942400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"European Union\u2014Next Generation EU","award":["B93D21010860004"],"award-info":[{"award-number":["B93D21010860004"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,11,24]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Chronic myeloid leukemia (CML) persists due to leukemic stem cells, notably the CD26+ subset. We investigated correlations between circulating CD26+ leukemic stem cells (LSCs) and BCR::ABL1 transcripts in an extracellular vesicle\u2013enriched secretome (EVES) from plasma samples of 44 CML patients. EVES were characterized and BCR::ABL1 quantified via digital PCR. We observed an inverse correlation between CD26+LSC counts and EVES BCR::ABL1 levels, especially in deep molecular responders (DMR). CD26+LSCs were elevated in patients in treatment-free remission (TFR), while EVES BCR::ABL1 levels were higher in those receiving therapy. These findings suggest distinct dynamics between LSC populations and vesicle-mediated transcript release, with potential implications for CML monitoring and prognosis.<\/jats:p>","DOI":"10.1093\/stcltm\/szaf062","type":"journal-article","created":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T09:49:42Z","timestamp":1763977782000},"source":"Crossref","is-referenced-by-count":1,"title":["Tracking leukemic residuals: dissecting the inverse relationship between CD26+ stem cells and extracellular\n                    <i>BCR::ABL1<\/i>\n                    transcript in Chronic Myeloid Leukemia (CML)"],"prefix":"10.1093","volume":"14","author":[{"given":"Silvia","family":"Mutti","sequence":"first","affiliation":[{"name":"Unit of Blood Diseases and Bone Marrow Transplant, Department of Clinical and Experimental Sciences (DSCS), Universit\u00e0 di Brescia, ASST Spedali Civili , Brescia 25123,","place":["Italy"]},{"name":"Centro di Ricerca Emato-Oncologica AIL (CREA), ASST Spedali Civili , Brescia 25123,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessia","family":"Cavalleri","sequence":"additional","affiliation":[{"name":"Unit of Blood Diseases and Bone Marrow Transplant, Department of Clinical and Experimental Sciences (DSCS), Universit\u00e0 di Brescia, ASST Spedali Civili , Brescia 25123,","place":["Italy"]},{"name":"Centro di Ricerca Emato-Oncologica AIL (CREA), ASST Spedali Civili , Brescia 25123,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Sicuranza","sequence":"additional","affiliation":[{"name":"Azienda Ospedaliera Universitaria, University of Siena , Siena 53100,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Pacelli","sequence":"additional","affiliation":[{"name":"Azienda Ospedaliera Universitaria, University of Siena , Siena 53100,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Ielo","sequence":"additional","affiliation":[{"name":"Umberto I Policlinico, La Sapienza University , Rome 00161,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucia","family":"Paolini","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Specialties, Radiological Sciences and Public Health (DSMC), Universit\u00e0 di Brescia , Brescia, 25123,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentina","family":"Mangolini","sequence":"additional","affiliation":[{"name":"Department of Molecular and Translational Medicine (DMMT), Universit\u00e0 di Brescia , Brescia 25123,","place":["Italy"]},{"name":"IRCCS Fondazione Don Carlo Gnocchi ONLUS , Milan 20148,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro","family":"Leoni","sequence":"additional","affiliation":[{"name":"Unit of Blood Diseases and Bone Marrow Transplant, Department of Clinical and Experimental Sciences (DSCS), Universit\u00e0 di Brescia, ASST Spedali Civili , Brescia 25123,","place":["Italy"]},{"name":"Centro di Ricerca Emato-Oncologica AIL (CREA), ASST Spedali Civili , Brescia 25123,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teresa","family":"Miracapillo","sequence":"additional","affiliation":[{"name":"Azienda Ospedaliera Universitaria, University of Siena , Siena 53100,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camilla","family":"Turriziani","sequence":"additional","affiliation":[{"name":"Azienda Ospedaliera Universitaria, University of Siena , Siena 53100,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabetta","family":"Abruzzese","sequence":"additional","affiliation":[{"name":"Hematology, S. Eugenio Hospital , ASLRoma2 00144,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mirko","family":"Farina","sequence":"additional","affiliation":[{"name":"Unit of Blood Diseases and Bone Marrow Transplant, Department of Clinical and Experimental Sciences (DSCS), Universit\u00e0 di Brescia, ASST Spedali Civili , Brescia 25123,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annalisa","family":"Radeghieri","sequence":"additional","affiliation":[{"name":"Department of Molecular and Translational Medicine (DMMT), Universit\u00e0 di Brescia , Brescia 25123,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michele","family":"Malagola","sequence":"additional","affiliation":[{"name":"Unit of Blood Diseases and Bone Marrow Transplant, Department of Clinical and Experimental Sciences (DSCS), Universit\u00e0 di Brescia, ASST Spedali Civili , Brescia 25123,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Massimo","family":"Breccia","sequence":"additional","affiliation":[{"name":"Umberto I Policlinico, La Sapienza University , Rome 00161,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monica","family":"Bocchia","sequence":"additional","affiliation":[{"name":"Azienda Ospedaliera Universitaria, University of Siena , Siena 53100,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Domenico","family":"Russo","sequence":"additional","affiliation":[{"name":"Unit of Blood Diseases and Bone Marrow Transplant, Department of Clinical and Experimental Sciences (DSCS), Universit\u00e0 di Brescia, ASST Spedali Civili , Brescia 25123,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3494-2624","authenticated-orcid":false,"given":"Simona","family":"Bernardi","sequence":"additional","affiliation":[{"name":"Unit of Blood Diseases and Bone Marrow Transplant, Department of Clinical and Experimental Sciences (DSCS), Universit\u00e0 di Brescia, ASST Spedali Civili , Brescia 25123,","place":["Italy"]},{"name":"Centro di Ricerca Emato-Oncologica AIL (CREA), ASST Spedali Civili , Brescia 25123,","place":["Italy"]},{"name":"National Center for Gene Therapy and Drugs based on RNA Technology (CN3) , Padua 35122,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,11,24]]},"reference":[{"key":"2025112700563166200_szaf062-B1","doi-asserted-by":"crossref","first-page":"2191","DOI":"10.1002\/ajh.27443","article-title":"Chronic myeloid leukemia: 2025 update on diagnosis, therapy, and 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