{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T06:09:14Z","timestamp":1784786954982,"version":"3.55.0"},"reference-count":66,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T00:00:00Z","timestamp":1784505600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"JSPS KAKENHI","award":["JP18H02349"],"award-info":[{"award-number":["JP18H02349"]}]},{"name":"JSPS KAKENHI","award":["22J14623"],"award-info":[{"award-number":["22J14623"]}]},{"name":"JSPS KAKENHI","award":["22H02525"],"award-info":[{"award-number":["22H02525"]}]},{"name":"JSPS KAKENHI","award":["23K23790"],"award-info":[{"award-number":["23K23790"]}]},{"name":"JSPS KAKENHI","award":["26K01900"],"award-info":[{"award-number":["26K01900"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,7,20]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Red blood cell (RBC) transfusions are essential for treating various medical conditions, but global demand is difficult to meet due to a dwindling donor pool and compatibility issues. Pluripotent stem cells (PSCs) offer a promising alternative of blood dependent on volunteer donors for RBC production, and dogs serve as an excellent model for translational research due to their physiological and genetic similarities to humans.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Canine induced pluripotent stem cells (ciPSCs) were differentiated toward hematopoietic and erythroid lineages. Differentiated cells were evaluated for hematopoietic marker expression, hemoglobinization, colony-forming capacity, enucleation, and hemoglobin gene expression. Glycophorin A (GYPA)-enhanced green fluorescent protein (EGFP) reporter ciPSC lines were generated using clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9\u2013mediated genome editing to visualize GYPA expression during differentiation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>This study introduces a protocol for RBC differentiation using ciPSCs. We achieved generation of hemoglobinized RBCs, progressing through polychromatic and orthochromatic erythroblast\u2013like stages. CiPSC\u2013derived hematopoietic cells\/RBCs were confirmed to have immature characteristics as determined by limited colony-forming capacities, low enucleation, and embryonic and fetal hemoglobin gene expression. Additionally, we created GYPA-EGFP reporter ciPSC lines using CRISPR-Cas9\u2013mediated genome editing, enabling real-time visualization of GYPA expression. This innovation confirmed GYPA as a viable surface marker for ciPSC-derived RBCs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our findings mark an initial step toward establishing a canine PSC\u2013based erythroid differentiation system, providing a foundation for future improvements and exploration of applications for canine PSC\u2013derived RBCs.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/stcltm\/szag058","type":"journal-article","created":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T00:02:31Z","timestamp":1784505751000},"source":"Crossref","is-referenced-by-count":0,"title":["Red blood cell differentiation using canine-induced pluripotent stem cells"],"prefix":"10.1093","volume":"15","author":[{"given":"Kazuto","family":"Kimura","sequence":"first","affiliation":[{"name":"Laboratory of Cell Pathobiology, Department of Advanced Pathobiology, 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