{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T13:31:29Z","timestamp":1783431089609,"version":"3.54.6"},"reference-count":46,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2024,4,13]],"date-time":"2024-04-13T00:00:00Z","timestamp":1712966400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Health Innovation Project of Chinese Academy of Medical Sciences","award":["2021-I2M-1-052"],"award-info":[{"award-number":["2021-I2M-1-052"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81873666"],"award-info":[{"award-number":["81873666"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,6,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Microtia is a congenital auricle dysplasia with a high incidence and tissue engineering technology provides a promising strategy to reconstruct auricles. We previously described that the engineered cartilage constructed from microtia chondrocytes exhibited inferior levels of biochemical and biomechanical properties, which was proposed to be resulted of the decreased migration ability of microtia chondrocytes. In the current study, we found that Rho GTPase members were deficient in microtia chondrocytes. By overexpressing RhoA, Rac1, and CDC42, respectively, we further demonstrated that RhoA took great responsibility for the decreased migration ability of microtia chondrocytes. Moreover, we constructed PGA\/PLA scaffold-based cartilages to verify the chondrogenic ability of RhoA overexpressed microtia chondrocytes, and the results showed that overexpressing RhoA was of limited help in improving the quality of microtia chondrocyte engineered cartilage. However, coculture of adipose-derived stem cells (ADSCs) significantly improved the biochemical and biomechanical properties of engineered cartilage. Especially, coculture of RhoA overexpressed microtia chondrocytes and ADSCs produced an excellent effect on the wet weight, cartilage-specific extracellular matrix, and biomechanical property of engineered cartilage. Furthermore, we presented that coculture of RhoA overexpressed microtia chondrocytes and ADSCs combined with human ear-shaped PGA\/PLA scaffold and titanium alloy stent fabricated by CAD\/CAM and 3D printing technology effectively constructed and maintained auricle structure in vivo. Collectively, our results provide evidence for the essential role of RhoA in microtia chondrocytes and a developed strategy for the construction of patient-specific tissue-engineered auricular cartilage.<\/jats:p>","DOI":"10.1093\/stmcls\/sxae026","type":"journal-article","created":{"date-parts":[[2024,4,12]],"date-time":"2024-04-12T15:12:33Z","timestamp":1712934753000},"page":"554-566","source":"Crossref","is-referenced-by-count":6,"title":["Co-culture of RhoA-overexpressed microtia chondrocytes and adipose-derived stem cells in the construction of tissue-engineered ear-shaped cartilage"],"prefix":"10.1093","volume":"42","author":[{"given":"Yi","family":"Wu","sequence":"first","affiliation":[{"name":"Research Center of Plastic Surgery Hospital, Chinese Academy of Medical Science & Peking Union Medical College , Beijing 100144 , People\u2019s Republic of China"},{"name":"Key Laboratory of External Tissue and Organ Regeneration, Chinese Academy of Medical Sciences & Peking Union Medical 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100144 , People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kang","family":"Wang","sequence":"additional","affiliation":[{"name":"Research Center of Plastic Surgery Hospital, Chinese Academy of Medical Science & Peking Union Medical College , Beijing 100144 , People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zonglin","family":"Huang","sequence":"additional","affiliation":[{"name":"Research Center of Plastic Surgery Hospital, Chinese Academy of Medical Science & Peking Union Medical College , Beijing 100144 , People\u2019s Republic of China"},{"name":"Key Laboratory of External Tissue and Organ Regeneration, Chinese Academy of Medical Sciences & Peking Union Medical College , Beijing 100144 , People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Wang","sequence":"additional","affiliation":[{"name":"Research Center of Plastic Surgery Hospital, Chinese Academy of Medical Science & Peking Union Medical College , Beijing 100144 , People\u2019s Republic of China"},{"name":"Key Laboratory of External Tissue and Organ Regeneration, Chinese Academy of Medical Sciences & Peking Union Medical College , Beijing 100144 , People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Fu","sequence":"additional","affiliation":[{"name":"Research Center of Plastic Surgery Hospital, Chinese Academy of Medical Science & Peking Union Medical College , Beijing 100144 , People\u2019s Republic of China"},{"name":"Key Laboratory of External Tissue and Organ Regeneration, Chinese Academy of Medical Sciences & Peking Union Medical College , Beijing 100144 , People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiyue","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Auricular Reconstruction, Plastic Surgery Hospital, Chinese Academy of Medical Science & Peking Union Medical College , Beijing 100144 , People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Auricular Reconstruction, Plastic Surgery Hospital, Chinese Academy of Medical Science & Peking Union Medical College , Beijing 100144 , People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1359-4237","authenticated-orcid":false,"given":"Ran","family":"Xiao","sequence":"additional","affiliation":[{"name":"Research Center of Plastic Surgery Hospital, Chinese Academy of Medical Science & Peking Union Medical College , Beijing 100144 , People\u2019s Republic of China"},{"name":"Key Laboratory of External Tissue and Organ Regeneration, Chinese Academy of Medical Sciences & Peking Union Medical College , Beijing 100144 , People\u2019s Republic of 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