{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T18:19:30Z","timestamp":1787681970741,"version":"build-2784847793"},"reference-count":48,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T00:00:00Z","timestamp":1785456000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico\/National Council","award":["88887.848340\/2023-00"],"award-info":[{"award-number":["88887.848340\/2023-00"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico\/National Council","award":["88887.908083\/2023-00"],"award-info":[{"award-number":["88887.908083\/2023-00"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo\/S\u00e3o Paulo Research Foundation","award":["2024\/08173-6"],"award-info":[{"award-number":["2024\/08173-6"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo\/S\u00e3o Paulo Research Foundation","award":["2021\/02216-7"],"award-info":[{"award-number":["2021\/02216-7"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo\/S\u00e3o Paulo Research Foundation","award":["2017\/23195-2"],"award-info":[{"award-number":["2017\/23195-2"]}]},{"name":"European Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,22]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Diabetic kidney disease (DKD), the leading cause of end-stage renal disease, involves injury across multiple renal compartments. Autophagy dysregulation is a key pathogenic mechanism and may reduce Klotho, a renoprotective protein diminished in DKD. This study evaluated whether bone marrow-derived mesenchymal stem cells (MSCs), combined with empagliflozin and calorie restriction, could modulate autophagy and preserve Klotho expression in DKD.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Male BTBRob\/ob mice, a leptin-deficient model that develops diabetes and DKD in a progressive manner, were assigned to experimental groups and euthanized at 14-15 or 18-20\u2009weeks. Kidney tissues were analyzed by qPCR, Western blot, and immunohistochemistry.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>MSCs therapy improved hyperglycemia in a time-dependent manner (P\u2009&amp;lt;\u2009.0001), decreased albuminuria, and modestly improved eGFR, though weight gain persisted. Treatment modulated LC3 protein expression in cortical and medullary regions (P\u2009&amp;lt;\u2009.05), suggesting attenuation of early autophagy hyperactivation. It also helped maintain Klotho expression, correlating with reduced oxidative stress (P\u2009&amp;lt;\u2009.05).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Overall, MSCs combined with empagliflozin and calorie restriction show promise as a translational approach for DKD, warranting further long-term preclinical studies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/stmcls\/sxag043","type":"journal-article","created":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T11:42:46Z","timestamp":1785498166000},"source":"Crossref","is-referenced-by-count":0,"title":["Mesenchymal stem cells and empagliflozin: a potential therapeutic approach for autophagy and Klotho modulation in diabetic kidney disease"],"prefix":"10.1093","volume":"44","author":[{"given":"Marcella L","family":"Franco","sequence":"first","affiliation":[{"name":"Albert Einstein Research and Education Institute, Hospital Israelita Albert Einstein , S\u00e3o Paulo, 05653-000,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephany","family":"Beyerstedt","sequence":"additional","affiliation":[{"name":"Albert Einstein Research and Education Institute, Hospital Israelita Albert Einstein , S\u00e3o Paulo, 05653-000,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno S","family":"Barbosa","sequence":"additional","affiliation":[{"name":"Albert Einstein Research and Education Institute, Hospital Israelita Albert Einstein , S\u00e3o Paulo, 05653-000,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tuany R","family":"Alves","sequence":"additional","affiliation":[{"name":"Albert Einstein Research and Education Institute, Hospital Israelita Albert Einstein , S\u00e3o Paulo, 05653-000,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrei F","family":"da Silva","sequence":"additional","affiliation":[{"name":"Albert Einstein Research and Education Institute, Hospital Israelita Albert Einstein , S\u00e3o Paulo, 05653-000,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melise O","family":"Mariano","sequence":"additional","affiliation":[{"name":"Albert Einstein Research and Education Institute, Hospital Israelita Albert Einstein , S\u00e3o Paulo, 05653-000,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alanah K G","family":"Carlos","sequence":"additional","affiliation":[{"name":"Albert Einstein Research and Education Institute, Hospital Israelita Albert Einstein , S\u00e3o Paulo, 05653-000,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaqueline C","family":"Arjona","sequence":"additional","affiliation":[{"name":"Albert Einstein Research and Education Institute, Hospital Israelita Albert Einstein , S\u00e3o 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