{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T21:08:11Z","timestamp":1789160891038,"version":"build-2803163510"},"reference-count":82,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["2002652"],"award-info":[{"award-number":["2002652"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Harry S Moss Heart Trust, Bank of America","award":["UTAUS-FA00002510"],"award-info":[{"award-number":["UTAUS-FA00002510"]}]},{"name":"Texas Biologics","award":["TXB-24-04"],"award-info":[{"award-number":["TXB-24-04"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,22]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Human induced pluripotent stem cell\u2013derived cardiomyocytes (hiPSC-CMs) enable cardiovascular disease modeling and regenerative research but exhibit substantial heterogeneity and incomplete metabolic maturation. Palmitate (PA) and oleate (OA) can promote aerobic maturation, although responses vary. Despite sex differences in adult cardiac metabolism, sex effects in hiPSC-CMs remain poorly defined.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We assessed sex-dependent metabolic and functional responses in hiPSC-CMs from three male and three female lines treated with PA or OA. Contractile function, aerobic metabolism, and transcriptomic profiles were evaluated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Sex differences were modest at baseline but pronounced after FA treatment. Male- and female-derived hiPSC-CMs responded differently to PA and OA across contractile, metabolic, and transcriptional measures, indicating that FA identity and sex jointly shape hiPSC-CM phenotypes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Sex contributes to hiPSC-CM heterogeneity and modulates responses to FA-based maturation. Incorporating sex-specific metabolic and functional regulation may improve the consistency and physiological relevance of hiPSC-CM maturation strategies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/stmcls\/sxag047","type":"journal-article","created":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T11:37:45Z","timestamp":1787053065000},"source":"Crossref","is-referenced-by-count":0,"title":["Donor sex modulates hiPSC-derived cardiomyocyte metabolic and functional response to fatty acids"],"prefix":"10.1093","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9946-0653","authenticated-orcid":false,"given":"Anna K","family":"McClain","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering, The University of Texas at Austin , Austin, TX 78712,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cody","family":"Callahan","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, The University of Texas at Austin , Austin, TX 78712,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saivee S","family":"Shelke","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, The University of Texas at Austin , Austin, TX 78712,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roni","family":"Shir","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, The University of Texas at Austin , Austin, TX 78712,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie E","family":"Givens","sequence":"additional","affiliation":[{"name":"Biomedical Engineering, University of Minnesota , Minneapolis, MN 55455,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7613-7490","authenticated-orcid":false,"given":"Brenda M","family":"Ogle","sequence":"additional","affiliation":[{"name":"Biomedical Engineering, University of Minnesota , Minneapolis, MN 55455,","place":["United States"]},{"name":"Department of Pediatrics, University of Minnesota , Minneapolis, MN 55454,","place":["United States"]},{"name":"Stem Cell Institute, University of Minnesota , Minneapolis, MN 55455,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janet","family":"Zoldan","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, The University of Texas at Austin , Austin, TX 78712,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,8,19]]},"reference":[{"key":"2026091116365500900_sxag047-B1","year":"2024"},{"key":"2026091116365500900_sxag047-B2","doi-asserted-by":"publisher","first-page":"669","DOI":"10.1016\/0022-2828(92)93381-s","article-title":"Ultrastructural quantitation of mitochondria and myofilaments in cardiac muscle from 10 different animal species including man","volume":"24","author":"Barth","year":"1992","journal-title":"J Mol Cell Cardiol."},{"key":"2026091116365500900_sxag047-B3","doi-asserted-by":"publisher","first-page":"699","DOI":"10.1016\/j.cjca.2021.02.005","article-title":"The importance of gender to understand sex differences in cardiovascular disease","volume":"37","author":"Connelly","year":"2021","journal-title":"Can J Cardiol."},{"key":"2026091116365500900_sxag047-B4","doi-asserted-by":"publisher","first-page":"42","DOI":"10.1016\/j.yjmcc.2021.04.010","article-title":"Matters of the heart: cellular sex differences","volume":"160","author":"Walker","year":"2021","journal-title":"J Mol Cell Cardiol"},{"key":"2026091116365500900_sxag047-B5","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1186\/s40001-022-00880-z","article-title":"Sex differences in heart: from basics to clinics","volume":"27","author":"Prajapati","year":"2022","journal-title":"Eur J Med Res"},{"key":"2026091116365500900_sxag047-B6","doi-asserted-by":"publisher","first-page":"303","DOI":"10.3389\/fphys.2022.831179","article-title":"Sex matters: a comprehensive comparison of female and male hearts","volume":"13","author":"St. Pierre","year":"2022","journal-title":"Front. Physiol"},{"key":"2026091116365500900_sxag047-B7","doi-asserted-by":"publisher","first-page":"573","DOI":"10.1016\/j.nuclcard.2007.03.001","article-title":"Sex differences in myocardial oxygen and glucose metabolism","volume":"14","author":"Peterson","year":"2007","journal-title":"J Nucl Cardiol."},{"key":"2026091116365500900_sxag047-B8","doi-asserted-by":"publisher","first-page":"603","DOI":"10.1161\/CIRCRESAHA.113.302095","article-title":"Cardiac metabolism and its interactions with contraction, growth, and survival of cardiomyocytes","volume":"113","author":"Kolwicz","year":"2013","journal-title":"Circ Res"},{"key":"2026091116365500900_sxag047-B9","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25021211","article-title":"Metabolic flexibility of the heart: the role of fatty acid metabolism in health, heart failure, and cardiometabolic diseases","volume":"25","author":"Actis Dato","year":"2024","journal-title":"Int J Mol Sci"},{"key":"2026091116365500900_sxag047-B10","doi-asserted-by":"publisher","first-page":"511","DOI":"10.1161\/CIRCULATIONAHA.124.071973","article-title":"Genes of the fatty acid oxidation pathway are upregulated in female as compared to male cardiomyocytes","volume":"151","author":"Talukdar","year":"2025","journal-title":"Circulation."},{"key":"2026091116365500900_sxag047-B11","doi-asserted-by":"publisher","first-page":"eadn3510","DOI":"10.1126\/sciadv.adn3510","article-title":"Sex in cardiovascular disease: why this biological variable should be considered in in vitro models","volume":"10","author":"McClain","year":"2024","journal-title":"Sci Adv."},{"key":"2026091116365500900_sxag047-B12","doi-asserted-by":"publisher","first-page":"162","DOI":"10.1038\/nprot.2012.150","article-title":"Directed cardiomyocyte differentiation from human pluripotent stem cells by modulating wnt\/\u03b2-catenin signaling under fully defined conditions","volume":"8","author":"Lian","year":"2013","journal-title":"Nat Protoc"},{"key":"2026091116365500900_sxag047-B13","doi-asserted-by":"publisher","first-page":"2238","DOI":"10.1038\/s41467-019-09831-5","article-title":"Functional cardiac fibroblasts derived from human pluripotent stem cells via second heart field progenitors","volume":"10","author":"Zhang","year":"2019","journal-title":"Nat Commun"},{"key":"2026091116365500900_sxag047-B14","doi-asserted-by":"publisher","first-page":"670","DOI":"10.1161\/CIRCRESAHA.120.318049","article-title":"Generation of vascular smooth muscle cells from induced pluripotent stem cells: methods, applications, and considerations","volume":"128","author":"Shen","year":"2021","journal-title":"Circ Res"},{"key":"2026091116365500900_sxag047-B15","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1186\/s13287-022-02924-x","article-title":"High-efficient serum-free differentiation of endothelial cells from human iPS cells","volume":"13","author":"Hamad","year":"2022","journal-title":"Stem Cell Res Ther"},{"key":"2026091116365500900_sxag047-B16","doi-asserted-by":"publisher","first-page":"1093","DOI":"10.1152\/physrev.00006.2004","article-title":"Myocardial substrate metabolism in the normal and failing heart","volume":"85","author":"Stanley","year":"2005","journal-title":"Physiol Rev"},{"key":"2026091116365500900_sxag047-B17","doi-asserted-by":"publisher","DOI":"10.3390\/cells8091095","article-title":"Fatty acid-treated induced pluripotent stem cell-derived human cardiomyocytes exhibit adult cardiomyocyte-like energy metabolism phenotypes","volume":"8","author":"Horikoshi","year":"2019","journal-title":"Cells."},{"key":"2026091116365500900_sxag047-B18","doi-asserted-by":"publisher","first-page":"613","DOI":"10.14348\/molcells.2018.0143","article-title":"Maturation of cardiomyocytes derived from human pluripotent stem cells: current strategies and limitations","volume":"41","author":"Jiang","year":"2018","journal-title":"Mol Cells."},{"key":"2026091116365500900_sxag047-B19","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1038\/s41569-019-0331-x","article-title":"Cardiomyocyte maturation: advances in knowledge and implications for regenerative medicine","volume":"17","author":"Karbassi","year":"2020","journal-title":"Nat Rev Cardiol"},{"key":"2026091116365500900_sxag047-B20","doi-asserted-by":"crossref","DOI":"10.1093\/stmcls\/sxaf038","article-title":"Healthy human induced pluripotent stem cell-derived cardiomyocytes exhibit sex dimorphism even without the addition of hormones","volume":"43","author":"Givens","year":"2025","journal-title":"Stem Cells."},{"key":"2026091116365500900_sxag047-B21","doi-asserted-by":"publisher","first-page":"1494","DOI":"10.1016\/j.bpj.2024.03.015","article-title":"Lipid droplets as substrates for protein phase separation","volume":"123","author":"Kamatar","year":"2024","journal-title":"Biophys J"},{"key":"2026091116365500900_sxag047-B22","doi-asserted-by":"publisher","first-page":"519","DOI":"10.1016\/j.stemcr.2021.01.018","article-title":"Maturation of pluripotent stem cell-derived cardiomyocytes enables modeling of human hypertrophic cardiomyopathy","volume":"16","author":"Knight","year":"2021","journal-title":"Stem Cell Reports."},{"key":"2026091116365500900_sxag047-B23","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1016\/s0008-6363(99)00263-1","article-title":"C. Cardiac fatty acid uptake and transport in health and disease","volume":"45","author":"Van Der Vusse","year":"2000","journal-title":"Cardiovasc Res."},{"key":"2026091116365500900_sxag047-B24","doi-asserted-by":"publisher","first-page":"238","DOI":"10.1109\/OJEMB.2024.3377461","article-title":"BeatProfiler: multimodal in vitro analysis of cardiac function enables machine learning classification of diseases and drugs","volume":"5","author":"Kim","year":"2024","journal-title":"IEEE Open J Eng Med Biol"},{"key":"2026091116365500900_sxag047-B25","doi-asserted-by":"publisher","first-page":"657","DOI":"10.1016\/j.stemcr.2019.08.013","article-title":"Fatty acids enhance the maturation of cardiomyocytes derived from human pluripotent stem cells","volume":"13","author":"Yang","year":"2019","journal-title":"Stem Cell Reports."},{"key":"2026091116365500900_sxag047-B26","doi-asserted-by":"publisher","first-page":"2210","DOI":"10.1194\/jlr.D073197","article-title":"Nile red quantifier: a novel and quantitative tool to study lipid accumulation in patient-derived circulating monocytes using confocal microscopy","volume":"58","author":"Schnitzler","year":"2017","journal-title":"J Lipid Res"},{"key":"2026091116365500900_sxag047-B27","doi-asserted-by":"publisher","first-page":"109038","DOI":"10.1016\/j.jneumeth.2020.109038","article-title":"A method for quantification of vesicular compartments within cells using 3D reconstructed confocal z-stacks: Comparison of ImageJ and Imaris to count early endosomes within basal forebrain cholinergic neurons","volume":"350","author":"Gautier","year":"2021","journal-title":"J Neurosci Methods."},{"key":"2026091116365500900_sxag047-B28","doi-asserted-by":"publisher","first-page":"276","DOI":"10.1038\/s41587-020-0439-x","article-title":"The nf-core framework for community-curated bioinformatics pipelines","volume":"38","author":"Ewels","year":"2020","journal-title":"Nat Biotechnol"},{"key":"2026091116365500900_sxag047-B29","unstructured":"Patel H , et al \u00a0nf-core\/rnaseq: nf-core\/rnaseq v3.19.0 - Tungsten Turtle. Preprint at 10.5281\/zenodo.15631172 (2025)."},{"key":"2026091116365500900_sxag047-B30","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1093\/bioinformatics\/bts635","article-title":"STAR: Ultrafast universal RNA-seq aligner","volume":"29","author":"Dobin","year":"2013","journal-title":"Bioinformatics."},{"key":"2026091116365500900_sxag047-B31","doi-asserted-by":"publisher","first-page":"417","DOI":"10.1038\/nmeth.4197","article-title":"Salmon provides fast and bias-aware quantification of transcript expression","volume":"14","author":"Patro","year":"2017","journal-title":"Nat Methods."},{"key":"2026091116365500900_sxag047-B32","doi-asserted-by":"publisher","DOI":"10.1093\/nargab\/lqaa078","article-title":"ComBat-seq: Batch effect adjustment for RNA-seq count data","volume":"2","author":"Zhang","year":"2020","journal-title":"NAR Genom Bioinform"},{"key":"2026091116365500900_sxag047-B33","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaf018","article-title":"edgeR v4: powerful differential analysis of sequencing data with expanded functionality and improved support for small counts and larger datasets","volume":"53","author":"Chen","year":"2025","journal-title":"Nucleic Acids Res"},{"key":"2026091116365500900_sxag047-B34","doi-asserted-by":"publisher","author":"Korotkevich","year":"2016","DOI":"10.1101\/060012"},{"key":"2026091116365500900_sxag047-B35","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/genetics\/iyad031","article-title":"The gene ontology knowledgebase in 2023","volume":"224","author":"The Gene Ontology Consortium","year":"2023","journal-title":"Genetics."},{"key":"2026091116365500900_sxag047-B36","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1038\/75556","article-title":"Gene ontology: tool for the unification of biology","volume":"25","author":"Ashburner","year":"2000","journal-title":"Nat Genet"},{"key":"2026091116365500900_sxag047-B37","author":"Subramanian","year":"2005"},{"key":"2026091116365500900_sxag047-B38","doi-asserted-by":"publisher","first-page":"417","DOI":"10.1016\/j.cels.2015.12.004","article-title":"The molecular signatures database hallmark gene set collection","volume":"1","author":"Liberzon","year":"2015","journal-title":"Cell Syst"},{"key":"2026091116365500900_sxag047-B39","doi-asserted-by":"publisher","first-page":"417","DOI":"10.1007\/s11307-019-01348-z","article-title":"Optical redox imaging of fixed unstained muscle slides reveals useful biological information","volume":"21","author":"Xu","year":"2019","journal-title":"Mol Imaging Biol"},{"key":"2026091116365500900_sxag047-B40","doi-asserted-by":"publisher","first-page":"244","DOI":"10.1038\/nature24265","article-title":"Landscape of X chromosome inactivation across human tissues","volume":"550","author":"Tukiainen","year":"2017","journal-title":"Nature."},{"key":"2026091116365500900_sxag047-B41","doi-asserted-by":"publisher","first-page":"E188","DOI":"10.1161\/CIRCRESAHA.122.321732","article-title":"BIN1, myotubularin, and dynamin-2 coordinate T-Tubule growth in cardiomyocytes","volume":"132","author":"Perdreau-Dahl","year":"2023","journal-title":"Circ Res"},{"key":"2026091116365500900_sxag047-B42","doi-asserted-by":"publisher","first-page":"800529","DOI":"10.3389\/fcell.2021.800529","article-title":"Recent advances in modeling mitochondrial cardiomyopathy using human induced pluripotent stem cells","volume":"9","author":"Pavez-Giani","year":"2021","journal-title":"Front Cell Dev Biol."},{"key":"2026091116365500900_sxag047-B43","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1093\/cvr\/cvac077","article-title":"Ryanodine receptor 2 (RYR2) dysfunction activates the unfolded protein response and perturbs cardiomyocyte maturation","volume":"119","author":"Guo","year":"2023","journal-title":"Cardiovasc Res"},{"key":"2026091116365500900_sxag047-B44","doi-asserted-by":"publisher","first-page":"e16063","DOI":"10.7717\/peerj.16063","article-title":"Evolution of protein domain repertoires of CALHM6","volume":"12","author":"Javed","year":"2024","journal-title":"PeerJ"},{"key":"2026091116365500900_sxag047-B45","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1161\/JAHA.121.021814","article-title":"Migraine-associated mutation in the Na, K-ATPase leads to disturbances in cardiac metabolism and reduced cardiac function","volume":"11","author":"Staehr","year":"2022","journal-title":"J Am Heart Assoc"},{"key":"2026091116365500900_sxag047-B46","doi-asserted-by":"publisher","first-page":"1395","DOI":"10.1172\/JCI8551","article-title":"CaM kinase signaling induces cardiac hypertrophy and activates the MEF2 transcription factor in vivo","volume":"105","author":"Passier","year":"2000","journal-title":"J Clin Invest."},{"key":"2026091116365500900_sxag047-B47","doi-asserted-by":"publisher","first-page":"144","DOI":"10.1186\/s13148-024-01755-2","article-title":"Distinct methylome profile of cfDNA in AMI patients reveals significant alteration in cAMP signaling pathway genes regulating cardiac muscle contraction","volume":"16","author":"Dash","year":"2024","journal-title":"Clin Epigenetics."},{"key":"2026091116365500900_sxag047-B48","doi-asserted-by":"publisher","first-page":"144","DOI":"10.1038\/s41436-018-0038-0","article-title":"MYLK pathogenic variants aortic disease presentation, pregnancy risk, and characterization of pathogenic missense variants","volume":"21","author":"Wallace","year":"2019","journal-title":"Genet Med."},{"key":"2026091116365500900_sxag047-B49","doi-asserted-by":"publisher","DOI":"10.3390\/ijms242316929","article-title":"Overcoming the variability of iPSCs in the manufacturing of Cell-Based therapies","volume":"24","author":"Nath","year":"2023","journal-title":"Int J Mol Sci"},{"key":"2026091116365500900_sxag047-B50","doi-asserted-by":"crossref","first-page":"924","DOI":"10.1016\/j.stemcr.2019.09.011","article-title":"Association of human iPSC gene signatures and X chromosome dosage with two distinct cardiac differentiation trajectories","volume":"13","author":"D\u2019Antonio-Chronowska","year":"2019","journal-title":"Stem Cell Reports."},{"key":"2026091116365500900_sxag047-B51","doi-asserted-by":"publisher","first-page":"101458","DOI":"10.1016\/j.scr.2019.101458","article-title":"Metabolic flux analyses to assess the differentiation of adult cardiac progenitors after fatty acid supplementation","volume":"38","author":"Malandraki-Miller","year":"2019","journal-title":"Stem Cell Res"},{"key":"2026091116365500900_sxag047-B52","doi-asserted-by":"publisher","first-page":"101560","DOI":"10.1016\/j.xpro.2022.101560","article-title":"A review of protocols for human iPSC culture, cardiac differentiation, subtype-specification, maturation, and direct reprogramming","volume":"3","author":"Lyra-Leite","year":"2022","journal-title":"STAR Protoc."},{"key":"2026091116365500900_sxag047-B53","doi-asserted-by":"publisher","first-page":"50","DOI":"10.3389\/fcell.2017.00050","article-title":"Naturally engineered maturation of cardiomyocytes","author":"Scuderi","year":"2017"},{"key":"2026091116365500900_sxag047-B54","doi-asserted-by":"publisher","first-page":"663","DOI":"10.1161\/CIRCGENETICS.117.001770","article-title":"Transcriptome and functional profile of cardiac myocytes is influenced by biological sex","volume":"10","author":"Trexler","year":"2017","journal-title":"Circ Cardiovasc Genet"},{"key":"2026091116365500900_sxag047-B55","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1152\/ajpheart.00073.2016","article-title":"Acute exposure to progesterone attenuates cardiac contraction by modifying myofilament calcium sensitivity in the female mouse heart","volume":"312","author":"Feridooni","year":"2017","journal-title":"Am J Physiol Heart Circ Physiol"},{"key":"2026091116365500900_sxag047-B56","doi-asserted-by":"publisher","first-page":"239","DOI":"10.1016\/j.jneumeth.2008.03.013","article-title":"NS21: Re-defined and modified supplement B27 for neuronal cultures","volume":"171","author":"Chen","year":"2008","journal-title":"J Neurosci Methods."},{"key":"2026091116365500900_sxag047-B57","doi-asserted-by":"publisher","first-page":"1614","DOI":"10.1161\/CIRCULATIONAHA.120.051921","article-title":"Sex-Specific control of human heart maturation by the progesterone receptor","volume":"143","author":"Sim","year":"2021","journal-title":"Circulation."},{"key":"2026091116365500900_sxag047-B58","doi-asserted-by":"publisher","first-page":"286","DOI":"10.1016\/j.bioactmat.2022.05.024","article-title":"Maturation of induced pluripotent stem cell-derived cardiomyocytes and its therapeutic effect on myocardial infarction in mouse","volume":"20","author":"Wu","year":"2023","journal-title":"Bioact. Mater"},{"key":"2026091116365500900_sxag047-B59","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1007\/s12551-024-01267-6","article-title":"Advancements in techniques for human iPSC-derived cardiomyocytes maturation: mechanical and electrical stimulation approaches","volume":"17","author":"Lu","year":"2025","journal-title":"Biophys Rev."},{"key":"2026091116365500900_sxag047-B60","doi-asserted-by":"publisher","first-page":"016101","DOI":"10.1063\/5.0269538","article-title":"Development of low-cost electrical stimulation device to promote hiPSC-CM differentiation and functionality","volume":"10","author":"Kalkunte","year":"2026","journal-title":"APL Bioeng"},{"key":"2026091116365500900_sxag047-B61","doi-asserted-by":"publisher","first-page":"875","DOI":"10.1089\/ars.2017.7451","article-title":"Evaluating cell metabolism through autofluorescence imaging of NAD(P)H and FAD","volume":"30","author":"Kolenc","year":"2019","journal-title":"Antioxid Redox Signal"},{"key":"2026091116365500900_sxag047-B62","doi-asserted-by":"publisher","first-page":"177","DOI":"10.1016\/j.tcb.2025.07.005","article-title":"NADH reductive stress drives metabolic reprogramming","volume":"36","author":"Yang","year":"2026","journal-title":"Trends Cell Biol"},{"key":"2026091116365500900_sxag047-B63","doi-asserted-by":"publisher","first-page":"594","DOI":"10.1038\/s41589-023-01460-w","article-title":"A genetically encoded tool to increase cellular NADH\/NAD+\u2009ratio in living cells","volume":"20","author":"Pan","year":"2024","journal-title":"Nat Chem Biol"},{"key":"2026091116365500900_sxag047-B64","doi-asserted-by":"publisher","first-page":"489","DOI":"10.1210\/er.2017-00211","article-title":"Metabolic flexibility as an adaptation to energy resources and requirements in health and disease","volume":"39","author":"Smith","year":"2018","journal-title":"Endocr Rev."},{"key":"2026091116365500900_sxag047-B65","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2020.584800","article-title":"Mitochondrial dynamics in adult cardiomyocytes and heart diseases","volume":"8","author":"Li","year":"2020","journal-title":"Front. Cell Dev. Biol"},{"key":"2026091116365500900_sxag047-B66","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1186\/s13287-021-02252-6","article-title":"Mitochondria and metabolic transitions in cardiomyocytes: lessons from development for stem cell-derived cardiomyocytes","volume":"12","author":"Garbern","year":"2021","journal-title":"Stem Cell Res Ther"},{"key":"2026091116365500900_sxag047-B67","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1161\/CIRCRESAHA.117.311307","article-title":"Mitochondrial reactive oxygen species in lipotoxic hearts induce post-translational modifications of AKAP121, DRP1, and OPA1 that promote mitochondrial fission","volume":"122","author":"Tsushima","year":"2018","journal-title":"Circ Res"},{"key":"2026091116365500900_sxag047-B68","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1016\/s0952-3278(97)90491-9","article-title":"Long-term effects of fatty acids on cell viability and gene expression of neonatal cardiac myocytes","volume":"57","author":"Van Bilsen","year":"1997","journal-title":"Prostaglandins Leukot Essent Fatty Acids"},{"key":"2026091116365500900_sxag047-B69","doi-asserted-by":"publisher","first-page":"104948","DOI":"10.1016\/j.tiv.2020.104948","article-title":"Palmitate-induced toxicity is associated with impaired mitochondrial respiration and accelerated oxidative stress in cultured cardiomyocytes: the critical role of coenzyme Q9\/10","volume":"68","author":"Dludla","year":"2020","journal-title":"Toxicology In Vitro"},{"key":"2026091116365500900_sxag047-B70","doi-asserted-by":"publisher","first-page":"1648","DOI":"10.1016\/j.bbalip.2014.09.012","article-title":"Sequestration of fatty acids in triglycerides prevents endoplasmic reticulum stress in an in vitro model of cardiomyocyte lipotoxicity","volume":"1841","author":"Bosma","year":"2014","journal-title":"Biochim Biophys Acta"},{"key":"2026091116365500900_sxag047-B71","doi-asserted-by":"publisher","first-page":"3077","DOI":"10.1073\/pnas.0630588100","article-title":"Triglyceride accumulation protects against fatty acid-induced lipotoxicity","volume":"100","author":"Listenberger","year":"2003","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2026091116365500900_sxag047-B72","doi-asserted-by":"publisher","first-page":"775382","DOI":"10.3389\/fnut.2021.775382","article-title":"Differential effects of oleic and palmitic acids on lipid droplet-mitochondria interaction in the hepatic cell line HepG2","volume":"8","author":"Eynaudi","year":"2021","journal-title":"Front Nutr"},{"key":"2026091116365500900_sxag047-B73","doi-asserted-by":"publisher","DOI":"10.1242\/dev.183095","article-title":"X chromosome inactivation in human development","volume":"147","author":"Patrat","year":"2020","journal-title":"Development"},{"key":"2026091116365500900_sxag047-B74","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1016\/j.stem.2012.03.008","article-title":"Molecular signatures of human induced pluripotent stem cells highlight sex differences and cancer genes","volume":"11","author":"Anguera","year":"2012","journal-title":"Cell Stem Cell."},{"key":"2026091116365500900_sxag047-B75","doi-asserted-by":"publisher","first-page":"166","DOI":"10.1038\/s41556-025-01823-6","article-title":"Escape from X inactivation is directly modulated by xist noncoding RNA","volume":"28","author":"Hauth","year":"2026","journal-title":"Nat Cell Biol"},{"key":"2026091116365500900_sxag047-B76","doi-asserted-by":"publisher","first-page":"77","DOI":"10.7150\/jgen.8123","article-title":"Chromosome imbalance as a driver of sex disparity in disease","volume":"2","author":"Abramowitz","year":"2014","journal-title":"J Genomics."},{"key":"2026091116365500900_sxag047-B77","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1016\/j.ijcard.2014.07.004","article-title":"A monounsaturated fatty acid (oleic acid) modulates electrical activity in atrial myocytes with calcium and sodium dysregulation","volume":"176","author":"Lin","year":"2014","journal-title":"Int J Cardiol"},{"key":"2026091116365500900_sxag047-B78","doi-asserted-by":"publisher","first-page":"755","DOI":"10.1016\/j.yjmcc.2004.06.011","article-title":"Mechanism of oleic acid-induced gap junctional disassembly in rat cardiomyocytes","volume":"37","author":"Huang","year":"2004","journal-title":"J Mol Cell Cardiol"},{"key":"2026091116365500900_sxag047-B79","doi-asserted-by":"publisher","first-page":"106598","DOI":"10.1016\/j.vascn.2019.106598","article-title":"HiPSC-CMs from different sex and ethnic origin donors exhibit qualitatively different responses to several classes of pharmacological challenges","volume":"99","author":"Zeng","year":"2019","journal-title":"J Pharmacol Toxicol Methods."},{"key":"2026091116365500900_sxag047-B80","doi-asserted-by":"publisher","first-page":"132","DOI":"10.1016\/j.mito.2011.05.007","article-title":"Increased availability of endogenous and dietary oleic acid contributes to the upregulation of cardiac fatty acid oxidation","volume":"12","author":"Dobrzyn","year":"2012","journal-title":"Mitochondrion."},{"key":"2026091116365500900_sxag047-B81","doi-asserted-by":"publisher","first-page":"1406","DOI":"10.1016\/j.bbabio.2006.05.007","article-title":"Mitochondrial complex I: Structural and functional aspects","volume":"1757","author":"Lenaz","year":"2006","journal-title":"Biochim Biophys Acta"},{"key":"2026091116365500900_sxag047-B82","doi-asserted-by":"publisher","first-page":"569","DOI":"10.1016\/j.jare.2025.01.012","article-title":"Reductive stress: the key pathway in metabolic disorders induced by overnutrition","volume":"77","author":"Zhang","year":"2025","journal-title":"J Adv Res."}],"container-title":["Stem Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/stmcls\/advance-article-pdf\/doi\/10.1093\/stmcls\/sxag047\/70708901\/sxag047.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/stmcls\/article-pdf\/44\/9\/sxag047\/70708901\/sxag047.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/stmcls\/article-pdf\/44\/9\/sxag047\/70708901\/sxag047.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T20:37:08Z","timestamp":1789159028000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/stmcls\/article\/doi\/10.1093\/stmcls\/sxag047\/8766022"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,19]]},"references-count":82,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,8,22]]}},"URL":"https:\/\/doi.org\/10.1093\/stmcls\/sxag047","relation":{},"ISSN":["1066-5099","1549-4918"],"issn-type":[{"value":"1066-5099","type":"print"},{"value":"1549-4918","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026,9]]},"published":{"date-parts":[[2026,8,19]]},"article-number":"sxag047"}}