{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T23:32:53Z","timestamp":1793057573575,"version":"4.5.0"},"reference-count":45,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:00:00Z","timestamp":1790294400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"National Natural Science Foundation of China through the Young Scientists Fund","award":["32200655"],"award-info":[{"award-number":["32200655"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"crossref"}]},{"name":"National Natural Science Foundation of China through the Young Scientists Fund","award":["32270847"],"award-info":[{"award-number":["32270847"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Two-cell-like cells (2CLCs), a rare totipotent-like population within mouse embryonic stem cell (mESC) cultures, serve as a valuable model for studying early embryonic events like zygotic genome activation. However, the molecular mechanisms restraining their spontaneous emergence remain elusive.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Here, we identify Msl3, an essential component of the male-specific lethal complex that is required for H4K16ac deposition, as a key suppressor of 2C-like reprogramming. Msl3-mediated H4K16ac promotes the expression of LINE1 retrotransposons, which act as upstream repressors of the 2C-lineage master regulator Dux.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Consequently, Msl3 depletion reduces LINE1 expression, derepresses Dux, and robustly activates 2C-specific genes. Furthermore, LINE1 inhibition leads to heterochromatin dispersal and fragmented nucleoli.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our findings unveil a novel MSL3-H4K16ac-LINE1-Dux axis that actively safeguards mESC pluripotency by suppressing a latent 2C-like program.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/stmcls\/sxag056","type":"journal-article","created":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T11:11:08Z","timestamp":1790334668000},"source":"Crossref","is-referenced-by-count":0,"title":["<i>Msl3<\/i>\n                    -mediated H4K16ac suppresses 2C-like reprogramming by sustaining\n                    <i>LINE1<\/i>\n                    expression in mouse embryonic stem cells"],"prefix":"10.1093","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3657-3815","authenticated-orcid":false,"given":"Yuqing","family":"Zhu","sequence":"first","affiliation":[{"name":"Center for Stem Cell and Translational Medicine, School of Life Science and Medical Engineering, Anhui University , Hefei, Anhui 230601,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuo","family":"Cao","sequence":"additional","affiliation":[{"name":"Center for Stem Cell and Translational Medicine, School of Life Science and Medical Engineering, Anhui University , Hefei, Anhui 230601,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingying","family":"Wang","sequence":"additional","affiliation":[{"name":"Center for Stem Cell and Translational Medicine, School of Life Science and Medical Engineering, Anhui University , Hefei, Anhui 230601,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjing","family":"Sun","sequence":"additional","affiliation":[{"name":"Center for Stem Cell and Translational Medicine, School of Life Science and Medical Engineering, Anhui University , Hefei, Anhui 230601,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Song","sequence":"additional","affiliation":[{"name":"Center for Stem Cell and Translational Medicine, School of Life Science and Medical Engineering, Anhui University , Hefei, Anhui 230601,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shoudong","family":"Ye","sequence":"additional","affiliation":[{"name":"Center for Stem Cell and Translational Medicine, School of Life Science and Medical Engineering, Anhui University , Hefei, Anhui 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