{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T09:47:45Z","timestamp":1785232065864,"version":"3.55.0"},"reference-count":47,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2024,7,14]],"date-time":"2024-07-14T00:00:00Z","timestamp":1720915200000},"content-version":"vor","delay-in-days":195,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100005156","name":"Alexander von Humboldt-Stiftung","doi-asserted-by":"publisher","award":["1163897-NGA-GF-E"],"award-info":[{"award-number":["1163897-NGA-GF-E"]}],"id":[{"id":"10.13039\/100005156","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,17]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The global significance of processed seafood necessitates vigilant monitoring for health risks, particularly in the ready-to-eat (RTE) sector. This study assessed the microbiological safety and antimicrobial resistance (AMR) profiles of 520 RTE seafood samples collected from Bayelsa, Nigeria. Escherichia coli identification was conducted using culture-based and polymerase chain reaction (PCR) methods. The isolates were further characterized using standard bacteriological techniques. PCR screening was employed to detect virulence and resistance genes. Of the processed RTE seafood samples assessed, 12.1% tested positive for E. coli. Extended-spectrum beta-lactamase (ESBL)-producing E. coli accounted for 2.3% of the samples. Various diarrheagenic determinants were identified, with aggregative adherence regulator-activated island (aaiC) and attaching and effacing (eae) being the most prevalent. Higher AMR levels were observed in ESBL-producing strains. Additionally, extracellular virulence factors, biofilm formation, and hydrophobicity assays revealed diverse pathogenic potentials among the isolates. The detection of beta-lactamase AMR genes, such as blaTEM (15.9%), blaCTX\u2212M\u221215 (3.2%), and blaCTX\u2212M\u22121 (1.6%), underscores the genetic mechanisms responsible for resistance in E. coli strains recovered from RTE seafood. These findings underscore the need for thorough monitoring and strict control procedures to ensure the safety of RTE seafood and mitigate the risks associated with AMR in food consumption.<\/jats:p>","DOI":"10.1093\/sumbio\/qvae017","type":"journal-article","created":{"date-parts":[[2024,7,14]],"date-time":"2024-07-14T02:37:52Z","timestamp":1720924672000},"source":"Crossref","is-referenced-by-count":5,"title":["Microbiological safety and antimicrobial resistance profiles of ready-to-eat seafood in Bayelsa, Nigeria"],"prefix":"10.1093","volume":"1","author":[{"given":"Faith I","family":"Omeje","sequence":"first","affiliation":[{"name":"Applied Microbial Processes & Environmental Health Research Group, Faculty of Life Sciences, University of Benin , PMB 1154, Benin City, 300283 ,","place":["Nigeria"]},{"name":"Department of Microbiology, Federal University Otuoke , PMB 126, Yenagoa, 561502 Bayelsa ,","place":["Nigeria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9189-9258","authenticated-orcid":false,"given":"Abeni","family":"Beshiru","sequence":"additional","affiliation":[{"name":"Applied Microbial Processes & Environmental Health Research Group, Faculty of Life Sciences, University of Benin , PMB 1154, Benin City, 300283 ,","place":["Nigeria"]},{"name":"Department of Microbiology, College of Natural and Applied Sciences, Western Delta University , PMB 10, Oghara, 300104 ,","place":["Nigeria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isoken H","family":"Igbinosa","sequence":"additional","affiliation":[{"name":"Applied Microbial Processes & Environmental Health Research Group, Faculty of Life Sciences, University of Benin , PMB 1154, Benin City, 300283 ,","place":["Nigeria"]},{"name":"Department of Environmental Management and Toxicology, Faculty of Life Sciences, University of Benin , PMB 1154, Benin City, 300283 ,","place":["Nigeria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Etinosa O","family":"Igbinosa","sequence":"additional","affiliation":[{"name":"Applied Microbial Processes & Environmental Health Research Group, Faculty of Life Sciences, University of Benin , PMB 1154, Benin City, 300283 ,","place":["Nigeria"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,7,13]]},"reference":[{"key":"2024121909162300400_bib1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.13057\/bonorowo\/w100101","article-title":"Microbial quality of fish along with the Tilapia, African catfish and Sardinella artisanal value chains in Kpong and James Town, Ghana","volume":"10","author":"Aboagye","year":"2020","journal-title":"Int J Bonorowo Wetlands"},{"key":"2024121909162300400_bib2","doi-asserted-by":"crossref","first-page":"95","DOI":"10.4314\/ajcem.v20i2.3","article-title":"First report of enteropathogenic and entero-invasive Escherichia coli with multiple antibiotic resistance indices from African catfish (Clarias glariepinus) in Nigeria","volume":"20","author":"Akande","year":"2019","journal-title":"Afr J Clin Exp Microbiol"},{"key":"2024121909162300400_bib3","doi-asserted-by":"crossref","first-page":"1013","DOI":"10.1128\/aem.57.4.1013-1017.1991","article-title":"Detection of Escherichia coli and Shigella spp. in water by using the polymerase chain reaction and gene probes for uid","volume":"57","author":"Bej","year":"1991","journal-title":"Appl Environ Microb"},{"key":"2024121909162300400_bib5","doi-asserted-by":"crossref","first-page":"e0266059","DOI":"10.1371\/journal.pone.0266059","article-title":"Processed ready-to-eat (RTE) foods sold in Yenagoa, Nigeria, were colonized by diarrheagenic Escherichia coli, which constitute a probable hazard to human health","volume":"17","author":"Beshiru","year":"2022","journal-title":"PLoS One"},{"key":"2024121909162300400_bib4","doi-asserted-by":"crossref","first-page":"114913","DOI":"10.1016\/j.lwt.2023.114913","article-title":"Biofilm and antimicrobial resistance profile of extended-spectrum \u03b2-lactamase (ESBL) and AmpC \u03b2-lactamase producing Enterobacteriaceae in vegetables and salads","volume":"182","author":"Beshiru","year":"2023","journal-title":"LWT"},{"key":"2024121909162300400_bib6","first-page":"332","volume-title":"Performance Standards for Antimicrobial Susceptibility Testing; a CLSI Supplement for Global Application. 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