{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:07:42Z","timestamp":1753877262888,"version":"3.41.2"},"reference-count":48,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2024,9,25]],"date-time":"2024-09-25T00:00:00Z","timestamp":1727222400000},"content-version":"vor","delay-in-days":268,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,17]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>This study aimed to investigate the antibiotic resistance pattern, extended-spectrum \u03b2-lactamase (ESBL) production, and biofilm formation potential of Plesiomonas shigelloides in selected surface waters in southwestern Nigeria. Isolates were identified using the spread plate technique on inositol brilliant green bile agar and confirmed molecularly by polymerase chain reaction. Antibiotic susceptibility was determined using the Kirby\u2013Bauer disc diffusion method, ESBL production by the double disc synergistic test, and biofilm formation by the test tube method. A total of 111 P. shigelloides isolates were identified and confirmed. Significant resistance rates were observed against cefuroxime (71%), cefotaxime (92%), and ceftazidime (92%), while high susceptibility was noted for ciprofloxacin (94%), gentamicin (96%), and chloramphenicol (100%). A higher prevalence of multiple drug resistant phenotypes was observed during the dry season, reaching 79% and 60% along sampled rivers. Biofilm-producing phenotypes exhibited robust homogeneous biofilm formation. In this study, P. shigelloides exhibited high resistance to critical antibiotics such as third-generation cephalosporins. However, isolates displayed susceptibility to ciprofloxacin, gentamicin, and chloramphenicol. The presence of ESBL phenotypes and strong biofilm formation emphasizes the potential threat of P. shigelloides as an emerging environmental pathogen, highlighting the importance of monitoring and understanding antibiotic resistance patterns for effective public health management strategies.<\/jats:p>","DOI":"10.1093\/sumbio\/qvae027","type":"journal-article","created":{"date-parts":[[2024,9,25]],"date-time":"2024-09-25T02:34:31Z","timestamp":1727231671000},"source":"Crossref","is-referenced-by-count":0,"title":["Emerging antibiotic resistance in <i>Plesiomonasshigelloides<\/i>: insights from surface waters in Southwest Nigeria"],"prefix":"10.1093","volume":"1","author":[{"given":"Oluwarinu A","family":"Adepoju","sequence":"first","affiliation":[{"name":"Department of Microbiology, Obafemi Awolowo University , Ile-Ife 220103 ,","place":["Nigeria"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jumoke M","family":"Adefisoye","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Obafemi Awolowo University , Ile-Ife 220103 ,","place":["Nigeria"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ibukun M","family":"Adesiyan","sequence":"additional","affiliation":[{"name":"Department of Environmental and Occupational Health Science, University of Medical Science , Ondo, PMB 536 ,","place":["Nigeria"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2024,9,24]]},"reference":[{"key":"2024121909181468400_bib1","doi-asserted-by":"publisher","first-page":"632","DOI":"10.1016\/j.scitotenv.2019.02.062","article-title":"Incidence and antimicrobial susceptibility fingerprints of Plesiomonas shigelliodes isolates in water samples collected from some freshwater resources in Southwest Nigeria","volume":"665","author":"Adesiyan","year":"2019","journal-title":"Sci Total Environ"},{"key":"2024121909181468400_bib48_248_071524","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1128\/mr.59.1.143-169.1995","article-title":"Phylogenetic identification and in situ detection of individual microbial cells without cultivation","volume":"59","author":"Amann","year":"1995","journal-title":"Microbiol Rev"},{"key":"2024121909181468400_bib3","doi-asserted-by":"publisher","first-page":"877","DOI":"10.1093\/jac\/45.6.877","article-title":"\u03b2-lactamase expression in Plesiomonas shigelloides","volume":"45","author":"Avison","year":"2000","journal-title":"J Antimicrob Chemother"},{"key":"2024121909181468400_bib62_949_150724","doi-asserted-by":"crossref","first-page":"662","DOI":"10.1016\/j.jhazmat.2008.11.069","article-title":"Evaluation of arsenic and other physico-chemical parameters of surface and ground water of Jamshoro, Pakistan","volume":"166","author":"Baig","year":"2009","journal-title":"J Hazard Mater"},{"key":"2024121909181468400_bib6","doi-asserted-by":"publisher","first-page":"260","DOI":"10.1016\/j.copbio.2008.05.006","article-title":"Antibiotics and antibiotic resistance in water environments","volume":"19","author":"Baquero","year":"2008","journal-title":"Curr Opin Biotechnol"},{"key":"2024121909181468400_bib46_145_070724","doi-asserted-by":"crossref","first-page":"S347","DOI":"10.1038\/sj.bjp.0707607","article-title":"Plasmid encoded antibiotic resistance: acquisition and transfer of antibiotic resistance genes in bacteria","volume":"153","author":"Bennett","year":"2008","journal-title":"Br J Pharmacol"},{"key":"2024121909181468400_bib53_338_073124","first-page":"154","article-title":"Water quality indices used for surface water vulnerability assessment","volume":"2","author":"Bharti","year":"2011","journal-title":"Int J Environ Sci"},{"key":"2024121909181468400_bib7","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.ecoenv.2013.01.016","article-title":"Detection and fate of antibiotic resistant bacteria in wastewater treatment plants: a review","volume":"91","author":"Bouki","year":"2013","journal-title":"Ecotoxicol Environ Saf"},{"key":"2024121909181468400_bib52_626_072924","doi-asserted-by":"crossref","first-page":"1631","DOI":"10.1007\/s12665-009-0297-9","article-title":"Water quality assessment of the Jinshui River (China) using multivariate statistical techniques","volume":"60","author":"Bu","year":"2010","journal-title":"Environ Earth Sci"},{"key":"2024121909181468400_bib54_657_073424","doi-asserted-by":"crossref","first-page":"3657","DOI":"10.3390\/ijerph7103657","article-title":"Water microbiology. 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