{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T22:35:03Z","timestamp":1768084503298,"version":"3.49.0"},"reference-count":56,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2025,5,2]],"date-time":"2025-05-02T00:00:00Z","timestamp":1746144000000},"content-version":"vor","delay-in-days":121,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006142","name":"Nordic Energy Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006142","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,5,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Biofuel fermentation of plant materials by yeast typically requires supplementation with nutrients, such as vitamins and nitrogenous compounds, to enable yeast metabolic activity and growth. In this study, we used a simple milliliter-scale fermentation vial system to demonstrate that hydrolyzed whole-cell biomass from a photosynthetic cyanobacterium (Synechococcus sp. PCC 7002) can be used as a nutrient supplement for yeast fermentation of wheat straw and spruce. Adding lysozyme-treated, high-nitrogen cyanobacterial biomass (0.5% dry matter,\u00a0w\/v) to a Saccharomyces cerevisiae fermentation of wheat straw hydrolysate (\u223c30% dry matter, w\/w) increased the initial ethanol production rate from 6.7\u00a0\u00b1\u00a00.2 to 18.7\u00a0\u00b1\u00a01.0\u00a0g l\u22121 day\u22121, representing a nearly three-fold enhancement. Supplementation of an equivalent amount of acid-hydrolyzed cyanobacterial biomass increased ethanol production rates by only ~50%, indicating that essential nutrients were degraded during acid treatment. In addition, high-nitrogen cyanobacterial biomass improved initial ethanol production rates from wheat straw by 30%\u201380% compared to low-nitrogen biomass, suggesting that nitrogenous nutrients in the cyanobacteria were responsible for the enhanced yeast activity. Our data show that biomass from cyanobacteria can be used as a sustainable alternative to conventional nutrient supplements for biofuel fermentation of lignocellulosic biomass, including wheat straw and spruce hydrolysates.<\/jats:p>","DOI":"10.1093\/sumbio\/qvaf007","type":"journal-article","created":{"date-parts":[[2025,5,2]],"date-time":"2025-05-02T04:00:10Z","timestamp":1746158410000},"source":"Crossref","is-referenced-by-count":2,"title":["Whole-cell hydrolysate of cyanobacteria enhances biofuel fermentation of plant biomass"],"prefix":"10.1093","volume":"2","author":[{"given":"Valentina L","family":"Donati","sequence":"first","affiliation":[{"name":"University of Copenhagen Department of Biology, , 3000 Helsing\u00f8r ,","place":["Denmark"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benedikt M","family":"Blossom","sequence":"additional","affiliation":[{"name":"University of Copenhagen Department of Biology, , 3000 Helsing\u00f8r 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