{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,21]],"date-time":"2026-06-21T09:30:19Z","timestamp":1782034219952,"version":"3.54.5"},"reference-count":53,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2022,8,17]],"date-time":"2022-08-17T00:00:00Z","timestamp":1660694400000},"content-version":"vor","delay-in-days":228,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["MOST-108-2628-E-005-002-MY3"],"award-info":[{"award-number":["MOST-108-2628-E-005-002-MY3"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,8,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The strong transcriptional activity of the virulent gene pagA in Bacillus anthracis has been proven to be anthrax toxin activator (AtxA)-regulated. However, the obscure pagA transcription mechanism hinders practical applications of this strong promoter. In this study, a 509-bp DNA fragment [termed 509sequence, (\u2212508)-(+1) relative to the P2 transcription start site] was cloned upstream of rbs-GFPuv as pTOL02B to elucidate the AtxA-regulated transcription. The 509sequence was dissected into the \u221210 sequence, \u221235 sequence, ATrich tract, SLI\/SLII and upstream site. In conjunction with the heterologous co-expression of AtxA (under the control of the T7 promoter), the \u221210 sequence (TATACT) was sufficient for the AtxA-regulated transcription. Integration of pTOL02F\u2009+\u2009pTOLAtxA as pTOL03F showed that the AtxA-regulated transcription exhibited a strong specific fluorescence intensity\/common analytical chemistry term (OD600) of 40\u2009597\u2009\u00b1\u2009446 and an induction\/repression ratio of 122. An improved induction\/repression ratio of 276 was achieved by cultivating Escherichia coli\/pTOL03F in M9 minimal medium. The newly developed promoter system termed PAtxA consists of AtxA, the \u221210 sequence and Escherichia RNA polymerase. These three elements synergistically and cooperatively formed a previously undiscovered transcription system, which exhibited a tight-control, high-level, modulable and stationary-phase-specific transcription. The PAtxA was used for phaCAB expression for the stationary-phase polyhydroxybutyrate production, and the results showed that a PHB yield, content and titer of 0.20\u2009\u00b1\u20090.27\u2009g\/g-glucose, 68\u2009\u00b1\u200911% and 1.5\u2009\u00b1\u20090.4\u2009g\/l can be obtained. The positive inducible PAtxA, in contrast to negative inducible, should be a useful tool to diversify the gene information flow in synthetic biology.<\/jats:p>\n               <jats:p>Graphical Abstract<\/jats:p>\n               <jats:p\/>","DOI":"10.1093\/synbio\/ysac014","type":"journal-article","created":{"date-parts":[[2022,8,17]],"date-time":"2022-08-17T04:37:27Z","timestamp":1660711047000},"source":"Crossref","is-referenced-by-count":6,"title":["The transcription activator AtxA from <i>Bacillus anthracis<\/i> was employed for developing a tight-control, high-level, modulable and stationary-phase-specific transcription activity in <i>Escherichia coli<\/i>"],"prefix":"10.1093","volume":"7","author":[{"given":"Ying-Xing","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Chemical Engineering, National Chung Hsing University , Taichung, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Zhen","family":"Zhuo","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, National Chung Hsing University , Taichung, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8468-5366","authenticated-orcid":false,"given":"Si-Yu","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, National Chung Hsing University , Taichung, Taiwan"},{"name":"Innovation and Development Center of Sustainable Agriculture, National Chung Hsing University , Taichung, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,8,17]]},"reference":[{"key":"2022083005185483000_R1","doi-asserted-by":"publisher","first-page":"116","DOI":"10.1016\/j.gde.2005.02.007","article-title":"Transcriptional regulation by the numbers: models","volume":"15","author":"Bintu","year":"2005","journal-title":"Curr. 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