{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:34:19Z","timestamp":1787009659622,"version":"build-2736575974"},"reference-count":32,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2023,7,31]],"date-time":"2023-07-31T00:00:00Z","timestamp":1690761600000},"content-version":"vor","delay-in-days":211,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["DMR 2004875"],"award-info":[{"award-number":["DMR 2004875"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["1R01DK110770-01"],"award-info":[{"award-number":["1R01DK110770-01"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["DMR 2004875"],"award-info":[{"award-number":["DMR 2004875"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["1R01DK110770-01"],"award-info":[{"award-number":["1R01DK110770-01"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,8,18]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Maximizing protein secretion is an important target in the design of engineered living systems. In this paper, we characterize a trade-off between cell growth and per-cell protein secretion in the curli biofilm secretion system of Escherichia coli Nissle 1917. Initial characterization using 24-h continuous growth and protein production monitoring confirms decreased growth rates at high induction, leading to a local maximum in total protein production at intermediate induction. Propidium iodide (PI) staining at the endpoint indicates that cellular death is a dominant cause of growth reduction. Assaying variants with combinatorial constructs of inner and outer membrane secretion tags, we find that diminished growth at high production is specific to secretory variants associated with periplasmic stress mediated by outer membrane secretion and periplasmic accumulation of protein containing the outer membrane transport tag. RNA sequencing experiments indicate upregulation of known periplasmic stress response genes in the highly secreting variant, further implicating periplasmic stress in the growth\u2013secretion trade-off. Overall, these results motivate additional strategies for optimizing total protein production and longevity of secretory engineered living systems<\/jats:p>\n                  <jats:p>Graphical Abstract<\/jats:p>","DOI":"10.1093\/synbio\/ysad013","type":"journal-article","created":{"date-parts":[[2023,7,31]],"date-time":"2023-07-31T08:38:15Z","timestamp":1690792695000},"source":"Crossref","is-referenced-by-count":9,"title":["Periplasmic stress contributes to a trade-off between protein secretion and\u00a0cell growth in\u00a0\n                    <i>Escherichia coli<\/i>\n                    Nissle 1917"],"prefix":"10.1093","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6819-963X","authenticated-orcid":false,"given":"Sivaram Subaya","family":"Emani","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anton","family":"Kan","sequence":"additional","affiliation":[{"name":"Department of Materials, ETH Z\u00fcrich , Z\u00fcrich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy","family":"Storms","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Chemical Biology, Northeastern University , Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanna","family":"Bonanno","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, Northeastern University , Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jade","family":"Law","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Chemical Biology, Northeastern University , Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanhita","family":"Ray","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Karolinska Institutet , Stockholm, Sweden"},{"name":"Center for the Advancement of Integrated Medical and Engineering Sciences at Karolinska Institutet and KTH Royal Institute of Technology , Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neel S","family":"Joshi","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Chemical Biology, Northeastern University , Boston, MA, USA"},{"name":"Harvard Medical School , Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,7,31]]},"reference":[{"key":"2023081914162395000_R1","doi-asserted-by":"crossref","first-page":"1175","DOI":"10.1038\/s41578-021-00350-8","article-title":"Engineered living biomaterials","volume":"6","author":"Rodrigo-Navarro","year":"2021","journal-title":"Nat. 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Bioeng."},{"key":"2023081914162395000_R6","doi-asserted-by":"crossref","DOI":"10.1186\/s12866-018-1199-3","article-title":"Modelling the metabolism of\u00a0protein secretion through the Tat route in\u00a0Streptomyces lividans","volume":"18","author":"Valverde","year":"2018","journal-title":"BMC Microbiol."},{"key":"2023081914162395000_R7","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1038\/nmeth.4635","article-title":"Burden-driven feedback control of\u00a0gene expression","volume":"15","author":"Ceroni","year":"2018","journal-title":"Nat. Methods"},{"key":"2023081914162395000_R8","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1038\/nbt.3879","article-title":"Engineered bacteria can function in\u00a0the mammalian gut long-term as live diagnostics of\u00a0inflammation","volume":"35","author":"Riglar","year":"2017","journal-title":"Nat. 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