{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:34:59Z","timestamp":1787009699340,"version":"build-2736575974"},"reference-count":46,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2022,6,20]],"date-time":"2022-06-20T00:00:00Z","timestamp":1655683200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The objective of this experiment was to evaluate the effects of a multi-strain Bacillus subtilis-based direct-fed microbial (DFM) on nursery pig health as indicated by intestinal mucosal and blood plasma immunological markers and intestinal morphology. Eighty pigs, of equal number of barrows and gilts (initial BW: 7.0\u2005\u00b1\u20050.60 kg), weaned at 21\u2005\u00b1\u20051 d of age were randomly allotted to sixteen pens, with five pigs per pen. Two dietary treatments were implemented, a basal control (CON) and a basal control plus DFM (CDFM). Both diets were corn, soybean meal, and distillers dried grains based and were formulated to meet or exceed all nutritional requirements (NRC, 2012) and manufactured on site. Diets were fed for 42 d. On d 21 and 42 of the experiment, one pig per pen was randomly selected and euthanized, with equal number of males and females represented. Blood samples were collected prior to euthanasia for assessment of plasma concentrations of immunoglobulin A (IgA) and intestinal fatty acid binding protein. Segments of the gastrointestinal tract including duodenum, jejunum, ileum, ascending and distal colon were removed for analysis of intestinal morphology, and levels of interleukin 6, interleukin 10 (IL-10), and tumor necrosis factor alpha. Jejunal villus height was greater in the CDFM pigs as compared with CON pigs (P\u2005=\u20050.02) and ascending colon crypt depth tended to be greater on d 21 (P\u2005=\u20050.10). Compared to CON, CDFM significantly increased overall plasma IgA (P\u2005=\u20050.03) (0.58 vs. 0.73 0.05 mg\/mL, respectively), while it tended to increase plasma IgA (P\u2005=\u20050.06) on d 21 (0.34 vs. 0.54\u2005\u00b1\u20050.07 mg\/mL, respectively) and tended to increase overall IL-10 (P\u2005=\u20050.10) in the jejunum (113 vs. 195\u2005\u00b1\u200535 pg\/mL, respectively). Addition of a multi-strain Bacillus subtilis-based DFM may have an early benefit to nursery pig health status, observed through specific changes in morphology and both systemic and localized immunological markers.<\/jats:p>","DOI":"10.1093\/tas\/txac083","type":"journal-article","created":{"date-parts":[[2022,6,20]],"date-time":"2022-06-20T08:41:58Z","timestamp":1655714518000},"source":"Crossref","is-referenced-by-count":12,"title":["Effects of a multi-strain\n                    <i>Bacillus subtilis<\/i>\n                    -based direct-fed microbial on immunity markers and intestinal morphology in diets fed to weanling pigs"],"prefix":"10.1093","volume":"6","author":[{"given":"Jaron R","family":"Lewton","sequence":"first","affiliation":[{"name":"Department of Animal Science, Michigan State University , East Lansing, MI 48824 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrienne D","family":"Woodward","sequence":"additional","affiliation":[{"name":"United Animal Health , Sheridan, IN 46069 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronny L","family":"Moser","sequence":"additional","affiliation":[{"name":"United Animal Health , Sheridan, IN 46069 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6755-9800","authenticated-orcid":false,"given":"Kyan M","family":"Thelen","sequence":"additional","affiliation":[{"name":"Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University , East Lansing, MI 48824 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam J","family":"Moeser","sequence":"additional","affiliation":[{"name":"Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University , East Lansing, MI 48824 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie L","family":"Trottier","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Cornell University , Ithaca, NY 14853 , Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert J","family":"Tempelman","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Michigan State University , East Lansing, MI 48824 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dale W","family":"Rozeboom","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Michigan State University , East Lansing, MI 48824 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,6,20]]},"reference":[{"key":"2022071317482245800_CIT0001","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.anifeedsci.2013.01.002","article-title":"Effect of pelleting temperature and probiotic supplementation on growth performance and immune function of broilers fed maize\/soy-based diets","volume":"180","author":"Amerah","year":"2013","journal-title":"Anim. 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