{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T16:16:43Z","timestamp":1791562603290,"version":"4.3.4"},"reference-count":28,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2025,1,17]],"date-time":"2025-01-17T00:00:00Z","timestamp":1737072000000},"content-version":"vor","delay-in-days":16,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"DNA Genetics"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,1,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Enterotoxigenic Escherichia coli (ETEC) is a leading cause of postweaning diarrhea (PWD) and mortality of weaned pigs. The objective of this study was to evaluate genetic resistance of the polymorphism at nucleotide 307 (M307) in the FUT1 gene, to F18 E. coli infection considering different genotypes. A total of 179 pigs were used for this study. Pigs were genotyped for susceptibility to F18+ E. coli prior to the trial. Treatments included: genotype M307GA\u2014heterozygous for E. coli susceptibility (A), genotype M307GG\u2014homozygous E. coli susceptibility (B), or genotype M307AA\u2014homozygous for E. coli resistance (C). Pigs were weighed, assigned to pens based on genotype, and allowed to acclimate for 3 d prior to the challenge. On days 4, 5, and 6, pigs were inoculated intraorally at the oropharynx with an F18+ E. coli isolate at a geometric mean concentration of 9.8\u2005\u00d7\u2005109. Growth rate (average daily gain [ADG]), feed intake (average daily feed intake), and gain-to-feed ratio (G:F) were calculated by pen. All pigs were humanely euthanized at the end of the trial. Two fixed sections of ileum and distal jejunum were collected from a subpopulation and tested by in situ hybridization (ISH) to evaluate F18+ E. coli adherence. Fresh ileum samples were used for enumeration of F18, total E. coli, and total bacteria by real-time polymerase chain reaction. Mortality rates during the trial were 26.7% for genotype A, 18.3% for genotype B, and 0.0% for genotype C (P\u2005&amp;lt;\u20050.01). Starting weights prior to inoculation were not different (P\u2005=\u20050.29) among genotypes. Overall, pigs from genotype C grew 223\u00a0g\/d faster (P\u2005=\u20050.04) than genotype A. Pigs from genotype C tended to grow 185\u00a0g\/d faster (P\u2005=\u20050.09) than genotype B. G:F for genotype C (0.74) was 23% greater (P\u2005&amp;lt;\u20050.01) than G:F for genotype A (0.60) and tended to be 12% greater (P\u2005=\u20050.07) than genotype B (0.66). There were no differences in ADG or G:F between genotypes A and B. F18-specifc Cq units were decreased by 7.74 and 6.47 in genotypes A and B compared with genotype C (P\u2005\u2264\u20050.03). Signal by ISH was increased by 14.0-fold in genotype A compared with genotype C (P\u2005=\u20050.02). Adherence was not different among genotypes (P\u2005=\u20050.40). Genotype A had greater mortality and poorer growth performance than genotype B or C. Genotype C had no mortalities during the trial, grew faster, was more feed efficient, and had less F18 E. coli in the ileal mucosa compared with genotype A. Resistant genotypes provide an opportunity to reduce PWD and mortality due to an F18+ E. coli infection.<\/jats:p>","DOI":"10.1093\/tas\/txaf004","type":"journal-article","created":{"date-parts":[[2025,1,17]],"date-time":"2025-01-17T15:33:16Z","timestamp":1737127996000},"source":"Crossref","is-referenced-by-count":7,"title":["Maintained growth performance and reduced mortality of genetically resistant nursery pigs after an experimental virulent F18 enterotoxigenic <i>Escherichia coli<\/i> challenge"],"prefix":"10.1093","volume":"9","author":[{"given":"Michael W","family":"Welch","sequence":"first","affiliation":[{"name":"Carthage Veterinary Service Ltd , Carthage, IL 62321,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda J","family":"Cross","sequence":"additional","affiliation":[{"name":"DNA Genetics , Columbus, NE 68601,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iara D P","family":"Solar Diaz","sequence":"additional","affiliation":[{"name":"DNA Genetics , Columbus, NE 68601,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danielle C","family":"Johnson","sequence":"additional","affiliation":[{"name":"Carthage Veterinary Service Ltd , Carthage, IL 62321,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Parr","sequence":"additional","affiliation":[{"name":"Carthage Veterinary Service Ltd , Carthage, IL 62321,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom A","family":"Rathje","sequence":"additional","affiliation":[{"name":"DNA Genetics , Columbus, NE 68601,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Randy C","family":"Borg","sequence":"additional","affiliation":[{"name":"DNA Genetics , Columbus, NE 68601,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dustin D","family":"Boler","sequence":"additional","affiliation":[{"name":"Carthage Veterinary Service Ltd , Carthage, IL 62321,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,1,17]]},"reference":[{"key":"2025020608061570800_CIT0001","first-page":"73","article-title":"Presentation of postweaning Escherichia coli diarrhea in southern Ontario, prevalence of hemolytic E. coli serogroups involved, and their antimicrobial resistance patterns","volume":"66","author":"Amezcua","year":"2002","journal-title":"Can. J. Vet. Res"},{"key":"2025020608061570800_CIT0002","doi-asserted-by":"publisher","first-page":"684","DOI":"10.1080\/22221751.2019.1613176","article-title":"PCV3-associated disease in the United States swine herd","volume":"8","author":"Arruda","year":"2019","journal-title":"Emerg. Microbes Infect"},{"key":"2025020608061570800_CIT0003","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1093\/jas\/skaa113","article-title":"Effects of an F18 enterotoxigenic Escherichia coli challenge on growth performance, immunological status, and gastrointestinal structure of weaned pigs and the potential protective effect of direct-fed microbial blends","volume":"98","author":"Becker","year":"2020","journal-title":"J. Anim. Sci"},{"key":"2025020608061570800_CIT0004","doi-asserted-by":"publisher","first-page":"332","DOI":"10.1016\/j.vetmic.2007.02.007","article-title":"The age-dependent expression of the F18+ E. coli receptor on porcine gut epithelial cells is positively correlated with the presence of histo-blood group antigens","volume":"122","author":"Coddens","year":"2007","journal-title":"Vet. Microbiol"},{"key":"2025020608061570800_CIT0005","doi-asserted-by":"publisher","first-page":"469","DOI":"10.1007\/BF02214010","article-title":"The influence of the administration of sow\u2019s milk on the postweaning excretion of hemolytic E. coli in the pig","volume":"10","author":"Deprez","year":"1986","journal-title":"Vet. Res. Commun"},{"key":"2025020608061570800_CIT0006","doi-asserted-by":"publisher","first-page":"428","DOI":"10.1111\/age.12428","article-title":"Expression of key glycosphingolipid biosynthesis-globo series pathway genes in Escherichia coli F18-resistant and Escherichia coli F18-sensitive piglets","volume":"47","author":"Dong","year":"2016","journal-title":"Anim. Genet"},{"key":"2025020608061570800_CIT0007","doi-asserted-by":"publisher","first-page":"573","DOI":"10.3389\/fvets.2020.00573","article-title":"Synbiotic effects of enzyme and probiotics on intestinal health and growth of newly weaned pigs challenged with enterotoxigenic F18(+) Escherichia coli","volume":"7","author":"Duarte","year":"2020","journal-title":"Front. Vet. Sci"},{"key":"2025020608061570800_CIT0008","author":"Euken","year":"2021"},{"key":"2025020608061570800_CIT0009","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1002\/9781119350927.ch52","article-title":"Colibacillosis","volume-title":"Diseases of swine","author":"Fairbrother","year":"2019"},{"key":"2025020608061570800_CIT0010","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1079\/ahr2005105","article-title":"Escherichia coli in postweaning diarrhea in pigs: an update on bacterial types, pathogenesis, and prevention strategies","volume":"6","author":"Fairbrother","year":"2005","journal-title":"Anim. Health Res. Rev"},{"key":"2025020608061570800_CIT0011","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1016\/s0378-1135(02)00348-6","article-title":"Association between the porcine Escherichia coli F18 receptor genotype and phenotype and susceptibility to colonisation and postweaning diarrhoea caused by E. coli O138:F18","volume":"93","author":"Frydendahl","year":"2003","journal-title":"Vet. Microbiol"},{"key":"2025020608061570800_CIT0012","doi-asserted-by":"publisher","first-page":"e01913-20","DOI":"10.1128\/AEM.01913-20","article-title":"F4- and F18-positive enterotoxigenic Escherichia coli isolates from diarrhea of postweaning pigs: genomic characterization","volume":"86","author":"Garc\u00eda","year":"2020","journal-title":"Appl. Environ. Microbiol"},{"key":"2025020608061570800_CIT0013","doi-asserted-by":"publisher","first-page":"skae018","DOI":"10.1093\/jas\/skae018","article-title":"Zinc status and indicators of intestinal health in enterotoxigenic Escherichia coli F18 challenged newly weaned pigs fed diets with different levels of zinc","volume":"102","author":"Hansen","year":"2024","journal-title":"J. Anim. Sci"},{"key":"2025020608061570800_CIT0014","doi-asserted-by":"publisher","first-page":"198","DOI":"10.1016\/j.rvsc.2012.08.004","article-title":"Protection against neonatal Escherichia coli diarrhea by vaccination of sows with a novel multivalent vaccine candidate expressing E. coli adhesins associated with neonatal pig colibacillosis","volume":"94","author":"Hur","year":"2013","journal-title":"Res. Vet. Sci"},{"key":"2025020608061570800_CIT0015","doi-asserted-by":"publisher","first-page":"skad035","DOI":"10.1093\/jas\/skad035","article-title":"Efficacy of zinc glycinate reducing zinc oxide on intestinal health and growth of nursery pigs challenged with F18+ Escherichia coli","volume":"101","author":"Jang","year":"2023","journal-title":"J. Anim. Sci"},{"key":"2025020608061570800_CIT0016","doi-asserted-by":"publisher","first-page":"922","DOI":"10.2527\/jas.2008-0919","article-title":"Administration of Pediococcus acidilactici or Saccharomyces cerevisiae boulardii modulates development of porcine mucosal immunity and reduces intestinal bacterial translocation after Escherichia coli challenge","volume":"87","author":"Lessard","year":"2009","journal-title":"J. Anim. Sci"},{"key":"2025020608061570800_CIT0017","doi-asserted-by":"publisher","first-page":"736","DOI":"10.1007\/s003359900556","article-title":"Two alpha(1,2) fucosyltransferase genes on porcine chromosome 6q11 are closely linked to the blood group inhibitor (S) and Escherichia coli F18 receptor (ECF18R) loci","volume":"8","author":"Meijerink","year":"1997","journal-title":"Mamm. Genome"},{"key":"2025020608061570800_CIT0018","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1007\/s002510000263","article-title":"A DNA polymorphism influencing alpha(1,2)fucosyltransferase activity of the pig FUT1 enzyme determines susceptibility of small intestinal epithelium to Escherichia coli F18 adhesion","volume":"52","author":"Meijerink","year":"2000","journal-title":"Immunogenetics"},{"key":"2025020608061570800_CIT0019","doi-asserted-by":"publisher","first-page":"1616","DOI":"10.3390\/antibiotics12111616","article-title":"Antimicrobial resistance in commensal Escherichia coli of the porcine gastrointestinal tract","volume":"12","author":"O\u2019Neill","year":"2023","journal-title":"Antibiotics (Basel)"},{"key":"2025020608061570800_CIT0020","doi-asserted-by":"crossref","DOI":"10.54846\/am2024\/14","article-title":"Characterization of hemolytic E. coli and antimicrobial susceptibility from ISU-VDL submitted cases from 2010 to 2022","author":"Paiva","year":"2024"},{"key":"2025020608061570800_CIT0021","doi-asserted-by":"publisher","first-page":"1593","DOI":"10.1128\/CVI.05120-11","article-title":"A tripartite fusion, FaeG-FedF-LT(192)A2:B, of enterotoxigenic Escherichia coli (ETEC) elicits antibodies that neutralize cholera toxin, inhibit adherence of K88 (F4) and F18 fimbriae, and protect pigs against K88ac\/heat-labile toxin infection","volume":"18","author":"Ruan","year":"2011","journal-title":"Clin. Vaccine Immunol"},{"key":"2025020608061570800_CIT0022","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1038\/257135a0","article-title":"A genetic basis for resistance to enteric disease caused by E. coli","volume":"257","author":"Rutter","year":"1975","journal-title":"Nature"},{"key":"2025020608061570800_CIT0023","doi-asserted-by":"publisher","first-page":"574","DOI":"10.1136\/vr.95.25-26.574","article-title":"A possible basis for the breeding of pigs relatively resistant to neonatal diarrhoea","volume":"95","author":"Sellwood","year":"1974","journal-title":"Vet. Rec"},{"key":"2025020608061570800_CIT0024","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1093\/jas\/skaa314","article-title":"Dietary osteopontin-enriched algal protein as nutritional support in weaned pigs infected with F18-fimbriated enterotoxigenic Escherichia coli","volume":"98","author":"Smith","year":"2020","journal-title":"J. Anim. Sci"},{"key":"2025020608061570800_CIT0025","doi-asserted-by":"publisher","first-page":"1033","DOI":"10.3390\/microorganisms11041033","article-title":"Evaluating antimicrobial resistance trends in commensal Escherichia coli isolated from cecal samples of swine at slaughter in the United States, 2013-2019","volume":"11","author":"Sodagari","year":"2023","journal-title":"Microorganisms"},{"key":"2025020608061570800_CIT0026","doi-asserted-by":"publisher","first-page":"326","DOI":"10.1016\/j.aninu.2020.08.012","article-title":"Dietary inclusion of multispecies probiotics to reduce the severity of post-weaning diarrhea caused by Escherichia coli F18(+) in pigs","volume":"7","author":"Sun","year":"2021","journal-title":"Anim. Nutr"},{"key":"2025020608061570800_CIT0027","doi-asserted-by":"publisher","first-page":"520","DOI":"10.1128\/IAI.67.2.520-526.1999","article-title":"Receptor-dependent immune responses in pigs after oral immunization with F4 fimbriae","volume":"67","author":"Van den Broeck","year":"1999","journal-title":"Infect. Immun"},{"key":"2025020608061570800_CIT0028","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1093\/jas\/skae105","article-title":"Dietary monoglyceride supplementation to support intestinal integrity and host defenses in health-challenged weanling pigs","volume":"102","author":"White","year":"2024","journal-title":"J. Anim. Sci"}],"container-title":["Translational Animal Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/tas\/advance-article-pdf\/doi\/10.1093\/tas\/txaf004\/61488291\/txaf004.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/tas\/article-pdf\/doi\/10.1093\/tas\/txaf004\/61488291\/txaf004.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/tas\/article-pdf\/doi\/10.1093\/tas\/txaf004\/61488291\/txaf004.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,6]],"date-time":"2025-02-06T08:06:38Z","timestamp":1738829198000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/tas\/article\/doi\/10.1093\/tas\/txaf004\/7959545"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"references-count":28,"URL":"https:\/\/doi.org\/10.1093\/tas\/txaf004","relation":{},"ISSN":["2573-2102"],"issn-type":[{"value":"2573-2102","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2025]]},"published":{"date-parts":[[2025]]},"article-number":"txaf004"}}