{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,8]],"date-time":"2026-08-08T02:44:07Z","timestamp":1786157047874,"version":"build-2736575974"},"reference-count":23,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T00:00:00Z","timestamp":1784937600000},"content-version":"vor","delay-in-days":205,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,1,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Parasite control continues to be an important management consideration for small ruminant producers worldwide because of the increased cost associated with anthelmintic treatment and the growing risk of parasite resistance to commercially available anthelmintics. As an alternative, pumpkin seeds (PS) have demonstrated anthelmintic properties in vitro that may be applied on-farm to control parasitic infection. Therefore, the objective of these experiments was to evaluate lamb growth and monitor parasite burden in lambs offered PS supplemented growing diets by measuring lamb body weight (BW), dry matter intake (DMI), average daily gain (ADG), fecal egg count (FEC), FAMACHA eye score (FAM), and packed cell volume (PCV). We hypothesized that the supplementation of PS would decrease parasite burden in growing lambs. Analysis of data included fixed effects of pen and treatment, random effect of lamb, with repeated effect of week. In Experiment 1, all lambs were fed a common pelleted diet for 28\u2009days. The control group (CON) was supplemented with 0.21\u2009kg as fed\/hd\/day of whole shell corn while the PS group was supplemented with 0.45\u2009kg as fed\/hd\/day. These treatments were isocaloric and limit fed. At the conclusion of the supplementation period (d 28), lamb BW was lesser for those lambs offered PS when compared with CON (P\u2009&amp;lt;\u20090.01). In addition, there was evidence of compensatory gain as lamb ADG tended to be greater post-treatment for PS lambs when compared with CON lambs (P\u2009=\u20090.14). Lamb FAM and log10(FEC) did not differ between treatments (P\u2009&amp;gt;\u20090.05). However, lamb PCV was greater (P\u2009&amp;lt;\u20090.01) for PS supplemented lambs when compared with CON lambs during the duration of the supplementation period (Week 1\u20134). In Experiment 2, lambs were fed a common meal diet with differing concentrations of ground PS (0-g [A], 100-g [B], and 200-g [C], respectively) for 14\u2009days. Lamb BW at the conclusion of the supplementation period (d 14) and lamb ADG had linear effects whereas the concentration of PS in the diet increased, lamb BW decreased (P\u2009&amp;lt;\u20090.01). Additionally, there was no evidence of post-treatment compensatory growth (P\u2009&amp;lt;\u20090.33) and no differences in lamb PCV, log10FEC, and FAM (P\u2009&amp;gt;\u20090.12). Under the conditions of these experiments, animal growth decreased, and parasite burden remained unchanged for lamb offered PS. Additional research may be considered to better understand a preferred method of PS feed delivery that does not negatively impact lamb DMI and growth.<\/jats:p>","DOI":"10.1093\/tas\/txag112","type":"journal-article","created":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T11:41:43Z","timestamp":1784893303000},"source":"Crossref","is-referenced-by-count":0,"title":["The use of pumpkin seeds as an alternative anthelmintic to reduce parasite burden in naturally infected lambs"],"prefix":"10.1093","volume":"10","author":[{"given":"M","family":"Tacuri Vera","sequence":"first","affiliation":[{"name":"Department of Animal Sciences, The Ohio State University , Wooster, OH 44691,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Schramm","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, The Ohio State University , Columbus, OH 43210,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1288-3037","authenticated-orcid":false,"given":"A E","family":"Relling","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, The Ohio State University , Wooster, OH 44691,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B A","family":"Wenner","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, The Ohio State University , Columbus, OH 43210,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5103-0678","authenticated-orcid":false,"given":"B J","family":"Campbell","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, The Ohio State University , Wooster, OH 44691,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,7,25]]},"reference":[{"key":"2026080722165845700_txag112-B1","doi-asserted-by":"publisher","first-page":"100527","DOI":"10.1016\/j.ijpddr.2024.100527","article-title":"Revisiting anthelmintic resistance in sheep flocks from S\u00e3o Paulo State, Brazil","volume":"24","author":"Bassetto","year":"2024","journal-title":"Int. J. Parasitol. Drugs Drug Resist"},{"key":"2026080722165845700_txag112-B2","doi-asserted-by":"publisher","first-page":"100833","DOI":"10.1016\/j.vprsr.2023.100833","article-title":"Effect of commercial tannins on parasitic infection and immunity of lambs naturally infected with Haemonchus contortus","volume":"38","author":"Biz","year":"2023","journal-title":"Vet. Parasitol. Reg. Stud. Rep"},{"key":"2026080722165845700_txag112-B3","doi-asserted-by":"publisher","first-page":"457","DOI":"10.1186\/s13071-020-04329-2","article-title":"The threat of reduced efficacy of anthelmintics against gastrointestinal nematodes in sheep from an area considered anthelmintic resistance-free","volume":"13","author":"Bosco","year":"2020","journal-title":"Parasit. Vectors"},{"key":"2026080722165845700_txag112-B4","doi-asserted-by":"publisher","first-page":"txab113","DOI":"10.1093\/tas\/txab113","article-title":"The effects of protein supplementation and pasture maintenance on the growth, parasite burden, and economic return of pasture-raised lambs","volume":"5","author":"Campbell","year":"2021","journal-title":"Transl. Anim. Sci"},{"key":"2026080722165845700_txag112-B5","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1016\/j.smallrumres.2017.09.006","article-title":"The effects of alternative weaning strategies on lamb health and performance","volume":"156","author":"Campbell","year":"2017","journal-title":"Small Rumin. Res"},{"key":"2026080722165845700_txag112-B6","first-page":"1","article-title":"Effects of supplemental dried distillers grains or soybean hulls on growth and internal parasite status of grazing lambs","volume":"27","author":"Felix","year":"2012","journal-title":"Sheep Goat Res. J"},{"key":"2026080722165845700_txag112-B7","doi-asserted-by":"publisher","first-page":"364","DOI":"10.2527\/jas1970.312364x","article-title":"Taste responses in ruminants. III. Reactions of Pygmy goats, normal goats, sheep, and cattle to sucrose and sodium chloride","volume":"31","author":"Goatcher","year":"1970","journal-title":"J. Anim. Sci"},{"key":"2026080722165845700_txag112-B8","doi-asserted-by":"publisher","first-page":"18850","DOI":"10.1038\/s41598-022-23566-2","article-title":"Condensed tannins act as anthelmintics by increasing the rigidity of the nematode cuticle","volume":"12","author":"Greiffer","year":"2022","journal-title":"Sci. Rep"},{"key":"2026080722165845700_txag112-B9","doi-asserted-by":"publisher","first-page":"1456","DOI":"10.3390\/ijms17091456","article-title":"Evaluation of anthelmintic activity and composition of pumpkin (Cucurbita pepo L.) seed extracts\u2014in vitro and in vivo studies","volume":"17","author":"Grzybek","year":"2016","journal-title":"Int. J. Mol. Sci"},{"key":"2026080722165845700_txag112-B10","doi-asserted-by":"publisher","first-page":"1960","DOI":"10.3390\/ani13121960","article-title":"Anthelmintic treatment of sheep and the role of parasite refugia in a local context","volume":"13","author":"H\u00f6glund","year":"2023","journal-title":"Animals"},{"key":"2026080722165845700_txag112-B11","doi-asserted-by":"publisher","first-page":"2994","DOI":"10.1021\/jm201451h","article-title":"Terpenes from Copaifera demonstrated in vitro antiparasitic and synergic activity","volume":"55","author":"Izumi","year":"2012","journal-title":"J. Med. Chem"},{"key":"2026080722165845700_txag112-B12","doi-asserted-by":"publisher","first-page":"78","DOI":"10.1051\/parasite\/2021076","article-title":"The effect of pumpkin seed cake and ground cloves (Syzygium aromaticum) supplementation on gastrointestinal nematode egg shedding in sheep","volume":"28","author":"Je\u017eek","year":"2021","journal-title":"Parasite"},{"key":"2026080722165845700_txag112-B13","doi-asserted-by":"publisher","first-page":"14499","DOI":"10.1038\/s41598-021-93911-4","article-title":"Anthelmintic resistance and homeostatic plasticity (Brugia malayi)","volume":"11","author":"Kashyap","year":"2021","journal-title":"Sci. Rep"},{"key":"2026080722165845700_txag112-B14","author":"Kendall","year":"2024"},{"key":"2026080722165845700_txag112-B15","doi-asserted-by":"publisher","first-page":"164","DOI":"10.1016\/j.ijpddr.2014.07.007","article-title":"Recent advances in candidate-gene and whole-genome approaches to the discovery of anthelmintic resistance markers and the description of drug\/receptor interactions","volume":"4","author":"Kotze","year":"2014","journal-title":"Int. J. Parasitol. Drugs Drug Resist"},{"key":"2026080722165845700_txag112-B16","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.smallrumres.2015.12.036","article-title":"Investigation of possible pumpkin seeds and ginger effects on gastrointestinal nematode infection indicators in meat goat kids and lambs","volume":"136","author":"Matthews","year":"2016","journal-title":"Small Rumin. Res"},{"key":"2026080722165845700_txag112-B17","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1016\/s1099-4831(08)00202-2","article-title":"Alkaloids with antiprotozoal activity","volume":"66","author":"Osorio","year":"2008","journal-title":"Alkaloids Chem. Biol"},{"key":"2026080722165845700_txag112-B18","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1016\/j.vetpar.2018.09.007","article-title":"Alarming levels of anthelmintic resistance against gastrointestinal nematodes in sheep in The Netherlands","volume":"262","author":"Ploeger","year":"2018","journal-title":"Vet. Parasitol"},{"key":"2026080722165845700_txag112-B19","doi-asserted-by":"publisher","first-page":"106078","DOI":"10.1016\/j.prevetmed.2023.106078","article-title":"Challenges and opportunities for reducing anthelmintic use in ruminant livestock systems: insights from a sheep farmer survey in France","volume":"221","author":"Sautier","year":"2023","journal-title":"Prev. Vet. Med"},{"key":"2026080722165845700_txag112-B20","author":"Shulaw","year":"2011"},{"key":"2026080722165845700_txag112-B21","doi-asserted-by":"publisher","first-page":"2477","DOI":"10.1080\/00021369.1980.10864337","article-title":"Bitter taste of oxidized fatty acids","volume":"44","author":"Usuki","year":"1980","journal-title":"Agric. Biol. Chem"},{"key":"2026080722165845700_txag112-B22","author":"VanHoy","year":"2025"},{"key":"2026080722165845700_txag112-B23","doi-asserted-by":"publisher","first-page":"1501","DOI":"10.3390\/ani12121501","article-title":"Effectiveness of anthelmintic treatments in small ruminants in Germany","volume":"12","author":"Voigt","year":"2022","journal-title":"Animals"}],"container-title":["Translational Animal Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/tas\/advance-article-pdf\/doi\/10.1093\/tas\/txag112\/70022823\/txag112.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/tas\/article-pdf\/doi\/10.1093\/tas\/txag112\/70022823\/txag112.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/tas\/article-pdf\/doi\/10.1093\/tas\/txag112\/70022823\/txag112.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,8]],"date-time":"2026-08-08T02:17:02Z","timestamp":1786155422000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/tas\/article\/doi\/10.1093\/tas\/txag112\/8741852"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":23,"URL":"https:\/\/doi.org\/10.1093\/tas\/txag112","relation":{},"ISSN":["2573-2102"],"issn-type":[{"value":"2573-2102","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026]]},"published":{"date-parts":[[2026]]},"article-number":"txag112"}}