{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T02:10:45Z","timestamp":1778551845046,"version":"3.51.4"},"reference-count":47,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2019,12,6]],"date-time":"2019-12-06T00:00:00Z","timestamp":1575590400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"MS Biotec, Inc."},{"name":"Zoetis Animal Health"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Forty-five beef cull cows [body weight (BW) = 503 \u00b1 58 kg; body condition score (BCS) = 2.1 \u00b1 0.6] were randomized into two treatments to compare the effects of oral drenching of no probiotic vs. 100 mL of Megasphaera elsdenii NCIMB 41125 (M. elsdenii culture; Lactipro Advance; 2 \u00d7 108 cfu\/mL; MS Biotec, Inc., Wamego, KS) on the realimentation of cull cows. The study featured a rapid 0-d step-up of concentrate-na\u00efve cull cows to a 90% concentrate diet (1.43 Mcal\/kg of NEg). The cows were finished for 35 d and were fitted with a wireless rumination tag (Allflex Flex Tag; SCR Engineers, Ltd, Netanya, Israel), which tracked head movement to record eating and chewing activity. Rumen morphometrics was recorded on the harvest floor, with each carcass assigned a rumenitis score, and a fragment of the cranial sac removed for further papillae analysis. An additional 23, thin, non-fed cull cows were harvested at the same abattoir to compare the effects of concentrate realimentation on ruminal morphometrics. Megasphaera elsdenii culture-drenched cattle registered a 13.3% increase in rumination time (39.27 min\/d, P = 0.03) during the first week of the trial compared to controls. A numerical rumination advantage for M. elsdenii culture-administered cattle was observed during week 2 of trial (P = 0.17), with no differences between treatments from weeks 3 to 5 (P \u2265 0.40). Subjective rumenitis evaluations approached a tendency (P = 0.12), with non-M. elsdenii culture-drenched concentrate-fed cattle logging twice the score of their day 0 cohorts (2.52 vs. 1.17) suggesting considerable lactic insults occurred to the ruminal epithelium in the short 35-d trial. Despite the short feeding duration, concentrate realimentation prompted a significant improvement in mean papillae area (P &amp;lt; 0.01). Among concentrate-fed treatments, M. elsdenii culture-drenched cattle posted superior absorptive surface area (P = 0.01) and a greater ratio of papillae area of absorptive surface area (P = 0.05), suggesting that M. elsdenii culture is favorably altering the ecology of the rumen and promoting papillae growth perhaps by mitigating lactate-driven pH drops. In conclusion, M. elsdenii culture application in a 0-d step-up protocol to finishing diets can help mitigate the effects of ruminal acidosis.<\/jats:p>","DOI":"10.1093\/tas\/txz185","type":"journal-article","created":{"date-parts":[[2019,12,6]],"date-time":"2019-12-06T02:43:55Z","timestamp":1575600235000},"page":"194-205","source":"Crossref","is-referenced-by-count":6,"title":["The influence of Megasphaera elsdenii on rumen morphometrics of cull cows immediately stepped up to a high-energy finishing diet"],"prefix":"10.1093","volume":"4","author":[{"given":"Jonathan C","family":"DeClerck","sequence":"first","affiliation":[{"name":"Department of Animal and Food Sciences, Texas Tech University, Lubbock, TX"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathan R","family":"Reeves","sequence":"first","affiliation":[{"name":"Department of Animal and Food Sciences, Texas Tech University, Lubbock, TX"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark F","family":"Miller","sequence":"first","affiliation":[{"name":"Department of Animal and Food Sciences, Texas Tech University, Lubbock, TX"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bradley J","family":"Johnson","sequence":"first","affiliation":[{"name":"Department of Animal and Food Sciences, Texas Tech University, Lubbock, TX"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gary A","family":"Ducharme","sequence":"first","affiliation":[{"name":"MS Biotec, Inc., Wamego, KS"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryan J","family":"Rathmann","sequence":"first","affiliation":[{"name":"Department of Animal and Food Sciences, Texas Tech University, Lubbock, TX"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2019,12,6]]},"reference":[{"key":"2020051902404358500_CIT0001","doi-asserted-by":"publisher","first-page":"1447","DOI":"10.3168\/jds.S0022-0302(97)76074-0","article-title":"Relationship between fermentation acid production in the rumen and the requirement for physically effective fiber","volume":"80","author":"Allen","year":"1997","journal-title":"J. Dairy Sci"},{"key":"2020051902404358500_CIT0002","volume-title":"Official methods of analysis","author":"AOAC","year":"1995","edition":"16"},{"key":"2020051902404358500_CIT0003","volume-title":"Megasphaera elsdenii strain and its uses","author":"ARC\/Kemira Phosphates","year":"2006"},{"key":"2020051902404358500_CIT0004","doi-asserted-by":"publisher","first-page":"1092","DOI":"10.2527\/jas.2012-5774","article-title":"Ruminant nutrition symposium: role of fermentation acid absorption in the regulation of ruminal pH","volume":"89","author":"Aschenbach","year":"2011","journal-title":"J. Anim. Sci"},{"key":"2020051902404358500_CIT0005","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1016\/j.anifeedsci.2007.05.002","article-title":"Modelling the implications of feeding strategy on rumen fermentation and functioning of the rumen wall","volume":"143","author":"Bannink","year":"2008","journal-title":"Anim. Feed Sci. Technol"},{"key":"2020051902404358500_CIT0006","doi-asserted-by":"publisher","first-page":"567","DOI":"10.1152\/physrev.1990.70.2.567","article-title":"Energy contributions of volatile fatty acids from the gastrointestinal tract in various species","volume":"70","author":"Bergman","year":"1990","journal-title":"Physiol. Rev"},{"key":"2020051902404358500_CIT0007","doi-asserted-by":"publisher","first-page":"1053","DOI":"10.1071\/AR9751053","article-title":"Whole wheat grain feeding of lambs. Effects of roughage and wheat grain mixtures","volume":"26","author":"Bigham","year":"1975","journal-title":"Aust. J. Agric. Res"},{"key":"2020051902404358500_CIT0008","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1079\/PNS2002215","article-title":"Molecular analysis of the effect of short-chain fatty acids on intestinal cell proliferation","volume":"62","author":"Blotti\u00e8re","year":"2003","journal-title":"Proc. Nutr. Soc"},{"key":"2020051902404358500_CIT0009","doi-asserted-by":"publisher","first-page":"4037","DOI":"10.2527\/jas.2010-3219","article-title":"Association of liver abnormalities with carcass grading performance and value","volume":"88","author":"Brown","year":"2010","journal-title":"J. Anim. Sci"},{"key":"2020051902404358500_CIT0010","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1128\/AEM.42.4.649-655.1981","article-title":"Role of Megasphaera elsdenii in the fermentation of dl-[2-C]lactate in the rumen of dairy cattle","volume":"42","author":"Counotte","year":"1981","journal-title":"Appl. Environ. Microbiol"},{"key":"2020051902404358500_CIT0012","doi-asserted-by":"crossref","first-page":"688","DOI":"10.11606\/issn.1678-4456.bjvras.2006.26579","article-title":"Ruminoreticulum and omasum participation in the bovine total absorptive proventricle surface","volume":"43","author":"Daniel","year":"2006","journal-title":"Braz. J. Vet. Res. Anim. Sci"},{"key":"2020051902404358500_CIT0013","first-page":"159","volume-title":"Influence of dietary roughage level and Megasphaera elsdenii on feedlot performance and carcass composition of thin cull beef cows fed for a lean market","author":"DeClerck","year":"2019"},{"key":"2020051902404358500_CIT0014","doi-asserted-by":"publisher","first-page":"385","DOI":"10.1079\/bjn19930041","article-title":"Absorption of volatile fatty acids from the rumen of lactating dairy cows as influenced by volatile fatty acid concentration, pH and rumen liquid volume","volume":"69","author":"Dijkstra","year":"1993","journal-title":"Br. J. Nutr"},{"key":"2020051902404358500_CIT0015","doi-asserted-by":"crossref","first-page":"116","DOI":"10.21423\/bovine-vol1985no20p116-120","article-title":"Adaptive changes of the ruminal mucosa and their functional and clinical significance","volume":"20","author":"Dirksen","year":"1985","journal-title":"Bovine Pract"},{"issue":"2","key":"2020051902404358500_CIT0016","doi-asserted-by":"crossref","first-page":"195","DOI":"10.4314\/sajas.v42i2.13","article-title":"Megasphaera elsdenii on the performance of steers adapting to a high-concentrate diet, using three or five transition diets","volume":"42","author":"Drouillard","year":"2012","journal-title":"S. Afr. J. Anim. Sci"},{"key":"2020051902404358500_CIT0017","doi-asserted-by":"publisher","first-page":"28","DOI":"10.1016\/j.meatsci.2008.06.013","article-title":"Colour of bovine subcutaneous adipose tissue: a review of contributory factors, associations with carcass and meat quality and its potential utility in authentication of dietary history","volume":"81","author":"Dunne","year":"2008","journal-title":"Meat Sci"},{"key":"2020051902404358500_CIT0018","doi-asserted-by":"publisher","first-page":"277","DOI":"10.2527\/asasann.2017.567","article-title":"567 ruminal characteristics and feedlot performance of feedlot steers during accelerated step-up to high-concentrate diets using Lactipro Advance","volume":"95","author":"Ellerman","year":"2017","journal-title":"J. Anim. Sci"},{"key":"2020051902404358500_CIT0019","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.anifeedsci.2010.02.002","article-title":"Effect of ruminal administration of the lactate-utilizing strain Megasphaera elsdenii (Me) NCIMB 41125 on abrupt or gradual transition from forage to concentrate diets","volume":"157","author":"Henning","year":"2010","journal-title":"Anim. Feed Sci. Technol"},{"key":"2020051902404358500_CIT0020","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.anifeedsci.2009.12.011","article-title":"The potential of Megasphaera elsdenii isolates to control ruminal acidosis","volume":"157","author":"Henning","year":"2010","journal-title":"Anim. Feed Sci. Technol"},{"key":"2020051902404358500_CIT0021","doi-asserted-by":"publisher","first-page":"60","DOI":"10.1093\/jas\/sky404.134","article-title":"Ruminal papillae morphology and volatile fatty acid profile of beef feedlot steers fed L. salivarius L28 in replacement for tylosin","volume":"96","author":"Hoffmann","year":"2018","journal-title":"J. Anim. Sci"},{"key":"2020051902404358500_CIT0022","first-page":"216","article-title":"Selective enrichment, isolation and characterization of fast growing acid-tolerant and ionophores-resistant lactate utilizers from rumen contents of animals on high-energy diets","volume-title":"Ruminant physiology \u2013 digestion, metabolism and effects of nutrition on reproduction and welfare","author":"Horn","year":"2009"},{"key":"2020051902404358500_CIT0023","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1292\/jvms1939.49.585","article-title":"Ultrasonogram of hepatic abscess in cattle inoculated with fusobacterium necrophorum","volume":"49","author":"Itabisashi","year":"1987","journal-title":"Nihon Juigaku Zasshi"},{"key":"2020051902404358500_CIT0024","unstructured":"Kettunen, A., P. H.Henning, C. H.Horn, S.Alaja, and J.Apajalahti. 2008. Capability of Megasphaera elsdenii strain NCIMB 41125 and other probiotics to prevent accumulation of lactic acid in a rumen model. INRA \u2013 RRI Symp., Abstract."},{"key":"2020051902404358500_CIT0025","doi-asserted-by":"publisher","first-page":"1336","DOI":"10.3168\/jds.S0022-0302(04)73283-X","article-title":"Development and analysis of a rumen tissue sampling procedure","volume":"87","author":"Lesmeister","year":"2004","journal-title":"J. Dairy Sci"},{"key":"2020051902404358500_CIT0051","doi-asserted-by":"crossref","unstructured":"Leeuw, J-K., F. K.Siebrits, P. H.Henning, and H. H.Meissner. 2009. Effect of Megasphaera elsdenii NCIMB 41125 drenching on health and performance of steers fed high and low roughage diets in the feedlot. S. Afr. J. Anim. Sci. 39:337\u2013348. doi:10.4314\/sajas.v39i4.51129","DOI":"10.4314\/sajas.v39i4.51129"},{"key":"2020051902404358500_CIT0026","doi-asserted-by":"publisher","first-page":"6812","DOI":"10.3168\/jds.2014-8947","article-title":"Characterization of peripartum rumination and activity of cows diagnosed with metabolic and uterine diseases","volume":"98","author":"Liboreiro","year":"2015","journal-title":"J. Dairy Sci"},{"key":"2020051902404358500_CIT0027","volume-title":"Investigation of the effects of a viable strain of Megasphaera elsdenii NCIMB 41125 on ruminal pH and ruminal concentrations of organic acids following a carbohydrate challenge [M.S. thesis] of M.R. McDaniel","author":"McDaniel","year":"2009"},{"key":"2020051902404358500_CIT0028","doi-asserted-by":"crossref","first-page":"79","DOI":"10.4314\/sajas.v40i2.57275","article-title":"Ruminal acidosis: a review with detailed reference to the controlling agent Megasphaera elsdenii NCIMB 41125","volume":"40","author":"Meissner","year":"2010","journal-title":"S. Afr. J. Anim. Sci"},{"key":"2020051902404358500_CIT0029","doi-asserted-by":"publisher","first-page":"1775","DOI":"10.2527\/jas.2011-4999","article-title":"Rumen morphometrics and the effect of digesta pH and volume on volatile fatty acid absorption","volume":"91","author":"Melo","year":"2013","journal-title":"J. Anim. Sci"},{"key":"2020051902404358500_CIT0030","volume-title":"Utilizing Lactipro (Megasphaera elsdenii NCIMB 41125) to accelerated adaptation of cattle to high-concentrate diets and improve the health of high-risk-calves [PhD Diss]","author":"Miller","year":"2013"},{"key":"2020051902404358500_CIT0031","doi-asserted-by":"publisher","first-page":"1830","DOI":"10.1071\/AN14193","article-title":"Effects of different doses of sodium monensin on rumen papillae and tissue histology of feedlot Nellore cattle","volume":"54","author":"Murillo","year":"2014","journal-title":"Anim. Prod. Sci"},{"key":"2020051902404358500_CIT0033","doi-asserted-by":"publisher","first-page":"E17","DOI":"10.3168\/jds.2006-478","article-title":"Ruminal acidosis in beef cattle: the current microbiological and nutritional outlook","volume":"90","author":"Nagaraja","year":"2007","journal-title":"J. Dairy Sci"},{"key":"2020051902404358500_CIT0034","volume-title":"Nutrient requirements of beef cattle","author":"NRC","year":"1996","edition":"7"},{"key":"2020051902404358500_CIT0035","volume-title":"Nutrient requirements of beef cattle","author":"NRC","year":"2016","edition":"8"},{"key":"2020051902404358500_CIT0037","doi-asserted-by":"publisher","first-page":"275","DOI":"10.2527\/1998.761275x","article-title":"Acidosis in cattle: a review","volume":"76","author":"Owens","year":"1998","journal-title":"J. Anim. Sci"},{"key":"2020051902404358500_CIT0038","first-page":"141","volume-title":"Effect of ionophore on rumen characteristics, gas production, and occurrence of bloat in cattle grazing winter wheat pasture","author":"Paisley","year":"1998"},{"key":"2020051902404358500_CIT0040","first-page":"E133","article-title":"Controlling variation in feed intake through bunk management","volume":"81","author":"Pritchard","year":"2003","journal-title":"J. Anim. Sci"},{"key":"2020051902404358500_CIT0041","unstructured":"Rasby, R., A.Stalker, and R.Funston. 2014. Body condition scoring beef cows: a tool for managing the nutrition program for beef herds. Extensionpublications.unl.edu. [accessed June 6, 2019].http:\/\/extensionpublications.unl.edu\/assets\/pdf\/ec281.pdf."},{"key":"2020051902404358500_CIT0042","first-page":"526","article-title":"Effect of the feeding pattern on rumen wall morphology of cows and sheep","volume":"43","author":"Resende J\u00fanior","year":"2006","journal-title":"Braz. J. Vet Res. Anim. Sci"},{"key":"2020051902404358500_CIT0043","doi-asserted-by":"publisher","first-page":"1119","DOI":"10.1126\/science.1058830","article-title":"Factors that alter rumen microbial ecology","volume":"292","author":"Russell","year":"2001","journal-title":"Science"},{"key":"2020051902404358500_CIT0044","doi-asserted-by":"publisher","first-page":"2648","DOI":"10.2527\/jas2016-0282","article-title":"Nutritional recommendations of feedlot consulting nutritionists: the 2015 New Mexico State and Texas Tech University survey","volume":"94","author":"Samuelson","year":"2016","journal-title":"J. Anim. Sci"},{"key":"2020051902404358500_CIT0045","doi-asserted-by":"publisher","first-page":"275","DOI":"10.1079\/pns19790049","article-title":"Carbohydrate fermentation in the rumen\u2013variations on a theme","volume":"38","author":"Sutton","year":"1979","journal-title":"Proc. Nutr. Soc"},{"issue":"1","key":"2020051902404358500_CIT0046","doi-asserted-by":"publisher","DOI":"10.4148\/2378-5977.1036","article-title":"Lactipro (Megasphaera elsdenii) increases ruminal pH and alters volatile fatty acids and lactate during transition to an 80% concentrate diet","volume":"1","author":"Thieszen","year":"2015","journal-title":"Kansas Agric. Exp. Stat. Res. Rep"},{"key":"2020051902404358500_CIT0047","first-page":"189","article-title":"Rumenitis in cattle","volume":"8","author":"Thomson","year":"1967","journal-title":"Can. Vet. J"},{"key":"2020051902404358500_CIT0048","volume-title":"United States standards for grades of carcass beef","author":"USDA","year":"1997"},{"key":"2020051902404358500_CIT0049","author":"Woerner","year":"2010"},{"key":"2020051902404358500_CIT0050","doi-asserted-by":"publisher","first-page":"57","DOI":"10.2527\/1996.74157x","article-title":"Hormonal status, metabolic changes, and resting metabolic rate in beef heifers undergoing compensatory growth","volume":"74","author":"Yambayamba","year":"1996","journal-title":"J. Anim. Sci"}],"container-title":["Translational Animal Science"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/tas\/advance-article-pdf\/doi\/10.1093\/tas\/txz185\/31234050\/txz185.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/tas\/article-pdf\/4\/1\/194\/33235167\/txz185.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/tas\/article-pdf\/4\/1\/194\/33235167\/txz185.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T18:10:35Z","timestamp":1695492635000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/tas\/article\/4\/1\/194\/5660952"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,12,6]]},"references-count":47,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,1,1]]}},"URL":"https:\/\/doi.org\/10.1093\/tas\/txz185","relation":{},"ISSN":["2573-2102"],"issn-type":[{"value":"2573-2102","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2020,1]]},"published":{"date-parts":[[2019,12,6]]}}}