{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T00:38:49Z","timestamp":1775695129566,"version":"3.50.1"},"reference-count":42,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2021,5,1]],"date-time":"2021-05-01T00:00:00Z","timestamp":1619827200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100006162","name":"Foundation for Science and Technology Development of the State of Pernambuco","doi-asserted-by":"publisher","award":["APQ-0562-2.01\/17"],"award-info":[{"award-number":["APQ-0562-2.01\/17"]}],"id":[{"id":"10.13039\/501100006162","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["424800\/2018-7"],"award-info":[{"award-number":["424800\/2018-7"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,6,14]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Bauhinia cheilantha (Fabaceae), known popularly as pata-de-vaca and moror\u00f3 has been largely recommended treating several diseases in folk medicine. However, information on safe doses and use is still scarce. The goal was to evaluate in-vitro antioxidant and antihemolytic and also acute and sub-acute toxicity effects of hydroalcoholic extract from B. cheilantha leaves (HaEBcl). The identification of the compounds in the HaEBcl was performed by ultra-performance liquid chromatography coupled with a diode array detector and quadrupole time-of-flight mass spectrometry. Antioxidant and hemolytic activity of HaEBcl was evaluated in vitro. To study acute toxicity, female mice received HaEBcl in a single dose of 300 and 2.000 mg\/kg. Later, sub-acute toxicity was introduced in both female and male mice by oral gavage at 300, 1000, or 2000 mg\/kg for 28 consecutive days. Hematological and biochemical profiles were created from the blood as well as from histological analysis of the liver. HaEBcl is rich in flavonoids (quercitrin and afzelin), has no hemolytic effects and moderate antioxidant effects in vitro. Acute toxicity evaluation showed that lethal dose (LD50) of HaEBcl was over 2000\u00a0mg\/kg. Sub-acute toxicity testing elicited no clinical signs of toxicity, morbidity, or mortality. The hematological and biochemical parameters discounted any chance of hepatic or kidney toxicity. Furthermore, histopathological data did not reveal any disturbance in liver morphology in treated mice. Results indicate that HaEBcl has no hemolytic and moderate antioxidant effects in vitro. In addition, HaEBcl dosage levels up to 2000\u00a0mg\/kg are nontoxic and can be considered safe for mammals.<\/jats:p>","DOI":"10.1093\/toxres\/tfab044","type":"journal-article","created":{"date-parts":[[2021,4,30]],"date-time":"2021-04-30T19:13:26Z","timestamp":1619810006000},"page":"613-626","source":"Crossref","is-referenced-by-count":5,"title":["Safety assessment of<i>Bauhinia cheilantha<\/i>Bong. Steud leaves extract: acute, sub-acute toxicity, antioxidant, and antihemolytic evaluations"],"prefix":"10.1093","volume":"10","author":[{"given":"Alanne Lucena","family":"de Brito","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Neuroendocrinologia e Metabolismo, Departamento de Fisiologia e Farmacologia, Centro de Bioci\u00eancias, Universidade Federal de Pernambuco, Avenida Prof. Moraes Rego, 1235, 50670-901, Recife, Pernambuco, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carla Mirele Tab\u00f3sa","family":"Quixabeira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Neuroendocrinologia e Metabolismo, Departamento de Fisiologia e Farmacologia, Centro de Bioci\u00eancias, Universidade Federal de Pernambuco, Avenida Prof. Moraes Rego, 1235, 50670-901, Recife, Pernambuco, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lidiane M\u00e2cedo Alves","family":"de Lima","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica, Universidade Federal Rural de Pernambuco, Rua Dom Manoel de Medeiros, S\/N, 52171-900, Recife, Pernambuco, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvana Tavares","family":"Paz","sequence":"additional","affiliation":[{"name":"Departamento de Patologia, Centro de Bioci\u00eancias, Universidade Federal de Pernambuco, Avenida Prof. Moraes Rego, 1235, 50670-901, Recife, Pernambuco, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayala Nara Pereira","family":"Gomes","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica, Universidade Federal Rural de Pernambuco, Rua Dom Manoel de Medeiros, S\/N, 52171-900, Recife, Pernambuco, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thiago Ant\u00f4nio","family":"de Souza Ara\u00fajo","sequence":"additional","affiliation":[{"name":"Departamento de sa\u00fade, Centro Universit\u00e1rio Maur\u00edcio de Nassau, Rua Jonathas de Vasconcelos, 92, 51021-140, Recife, Pernambuco, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulysses Paulino","family":"de Albuquerque","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Ecologia e Evolu\u00e7\u00e3o de Sistemas Socioecol\u00f3gicos, Departamento de Bot\u00eanica, Centro de Bioci\u00eancias, Universidade Federal de Pernambuco, Avenida Prof. Moraes Rego, 1235, 50670-901, Recife, Pernambuco, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dayane Aparecida","family":"Gomes","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Neuroendocrinologia e Metabolismo, Departamento de Fisiologia e Farmacologia, Centro de Bioci\u00eancias, Universidade Federal de Pernambuco, Avenida Prof. Moraes Rego, 1235, 50670-901, Recife, Pernambuco, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tania Maria Sarmento","family":"Silva","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica, Universidade Federal Rural de Pernambuco, Rua Dom Manoel de Medeiros, S\/N, 52171-900, Recife, Pernambuco, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eduardo Carvalho","family":"Lira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Neuroendocrinologia e Metabolismo, Departamento de Fisiologia e Farmacologia, Centro de Bioci\u00eancias, Universidade Federal de Pernambuco, Avenida Prof. Moraes Rego, 1235, 50670-901, Recife, Pernambuco, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2021,5,29]]},"reference":[{"key":"2021070810593512700_ref1","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1590\/0100-67622015000300003","article-title":"Population structure and spatial distribution of Bauhinia cheilantha (Bong.) 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