{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T01:33:08Z","timestamp":1790127188365,"version":"4.0.1"},"reference-count":37,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2022,8,30]],"date-time":"2022-08-30T00:00:00Z","timestamp":1661817600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100010210","name":"MOA Health Science Foundation","doi-asserted-by":"publisher","award":["2021E021"],"award-info":[{"award-number":["2021E021"]}],"id":[{"id":"10.13039\/501100010210","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,10,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Scutellaria barbata D. Don (SB) is a widely used herbal medicine in China, with various pharmacological effects such as anti-oxidation, anti-inflammation, and anti-cancer. This work is aimed to investigate the tumor-suppressive effect of SB in cervical cancer (CC) and to identify its underlying mechanisms.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and materials<\/jats:title>\n                    <jats:p>CC cell lines (HeLa and HT-3) were treated with different concentrations of SB chloroform extract (ECSB) (0, 0.2, 0.5\u00a0mg\/ml). MiR-195-5p and LOXL2 mRNA expression in CC cell lines and tissue samples was detected by quantitative real-time polymerase chain reaction. Cell counting kit-8 experiment was utilized to examine cell viability; TUNEL assay and Transwell experiment was executed to examine cell apoptosis, migration, and invasion. Western blotting experiments were implemented to detect LOXL2 protein expression. Bioinformatics and dual-luciferase reporter gene experiment were executed to examine the binding relationship between miR-195-5p and LOXL2.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>ECSB repressed the viability, migration, and invasion of HeLa and HT-3 cells, and promoted cell apoptosis in a dose-dependent manner. MiR-195-5p was remarkably under-expressed in CC tissues and cells, and ECSB up-regulated miR-195-5p expression. MiR-195-5p inhibitors partially counteracted the suppressive effects of ECSB on the malignant phenotypes of HeLa and HT-3 cells. LOXL2 was a downstream target of miR-195-5p, and ECSB up-modulated LOXL2 expression by specifically repressing miR-195-5p.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>SB restrains CC cell proliferation and metastasis and promotes cell apoptosis via miR-195-5p\/LOXL2, which may be a potential therapeutic agent for CC treatment.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/toxres\/tfac058","type":"journal-article","created":{"date-parts":[[2022,8,24]],"date-time":"2022-08-24T08:52:46Z","timestamp":1661331166000},"page":"804-811","source":"Crossref","is-referenced-by-count":12,"title":["<i>Scutellaria barbata D. Don<\/i>\n                    inhibits cervical cancer cell proliferation, migration, and invasion via miR-195-5p\/LOXL2 axis"],"prefix":"10.1093","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1751-7738","authenticated-orcid":false,"given":"Shouyu","family":"Xue","sequence":"first","affiliation":[{"name":"Xi\u2019an Medical University Clinical Medical School, , Xi\u2019an 710021, Shaanxi Province , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aimin","family":"Geng","sequence":"additional","affiliation":[{"name":"Chang\u2019 an Hospital Department of Urological Surgery, , Xi\u2019an 710016, Shaanxi Province , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Lian","sequence":"additional","affiliation":[{"name":"Xi\u2019an Medical University Clinical Medical School, , Xi\u2019an 710021, Shaanxi Province , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Liu","sequence":"additional","affiliation":[{"name":"Shaanxi Provincial Hospital of Chinese Medicine Department of Infectious Diseases, , Xi\u2019an 710003, Shaanxi Province , China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,8,30]]},"reference":[{"issue":"1","key":"2022110110351644400_ref1","doi-asserted-by":"crossref","first-page":"7","DOI":"10.3322\/caac.21551","article-title":"Cancer statistics, 2019","volume":"69","author":"Siegel","year":"2019","journal-title":"CA Cancer J Clin"},{"issue":"2","key":"2022110110351644400_ref2","first-page":"320","article-title":"Cervical cancer: screening, diagnosis and staging","volume":"21","author":"Tsikouras","year":"2016","journal-title":"J BUON"},{"key":"2022110110351644400_ref3","doi-asserted-by":"crossref","first-page":"iv262","DOI":"10.1093\/annonc\/mdy160","article-title":"Corrections to \u201ccervical cancer: ESMO clinical practice guidelines for diagnosis, treatment and follow-up\u201d","volume":"29","author":"Marth","year":"2018","journal-title":"Ann Oncol"},{"issue":"35","key":"2022110110351644400_ref4","doi-asserted-by":"crossref","first-page":"5802","DOI":"10.1200\/JCO.2008.16.4368","article-title":"Chemoradiotherapy for Cervical Cancer Meta-Analysis Collaboration. 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