{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T18:36:08Z","timestamp":1779906968044,"version":"3.53.1"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2023,8,7]],"date-time":"2023-08-07T00:00:00Z","timestamp":1691366400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100018617","name":"LiaoNing Revitalization Talents Program","doi-asserted-by":"crossref","award":["XLYC1808031"],"award-info":[{"award-number":["XLYC1808031"]}],"id":[{"id":"10.13039\/501100018617","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Liaoning Provincial Department of Education Research Foundation of China","award":["LJKZ0844"],"award-info":[{"award-number":["LJKZ0844"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,10,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Epidemiological inquiry reveals that neuroinflammation and mitochondrial dysfunction caused by PM2.5 exposure are associated with Alzheimer\u2019s disease. Nevertheless, the molecular mechanisms of mitochondrial dynamics and neuroinflammation induced by PM2.5 exposure remain elusive. In this study, our objective was to explore the impact of PM2.5 on mitochondrial dynamics and neuroinflammation, while also examining the reparative potential of scorpion venom heat-resistant synthetic peptide (SVHRSP).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Western blot and quantitative reverse transcription polymerase chain reaction (RT-qPCR) were employed to ascertain the protein and gene levels of IL-1\u03b2, IL-6, and TNF-\u03b1 in BV2 cells. The concentration of IL-6 in the supernatant of the BV2 cell culture was measured by enzyme-linked immunosorbent assay. For the assessment of mitochondrial homeostasis, western blot, RT-qPCR, and cellular immunohistochemistry methods were utilized to investigate the protein and gene levels of DRP1 and MFN-2 in HT22 cells. In the context of signal pathway analyses, western blot, RT-qPCR, and immunofluorescence techniques were employed to detect the protein and gene expressions of PGC-1\u03b1 and SIRT3 in HT22 cells, respectively. Following the transfection with siPGC-1\u03b1RNA, downstream proteins of PGC-1\u03b1\/SIRT3 pathway in HT22 cells were investigated by Western blot and RT-qPCR.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>The experimental findings demonstrated that exposure to PM2.5 exacerbated neuroinflammation, resulting in elevated levels of IL-1\u03b2, IL-6, and TNF-\u03b1. Furthermore, it perturbed mitochondrial dynamics, as evidenced by increased DRP1 expression and decreased MFN-2 expression. Additionally, dysfunction was observed in the PGC-1\u03b1\/SIRT3 signal pathway. However, intervention with SVHRSP ameliorated the cellular damage induced by PM2.5 exposure.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>SVHRSP alleviated neuroinflammation and mitochondrial dynamics imbalance induced by PM2.5 exposure by downregulating the PGC-1\u03b1\/SIRT3 signaling pathway.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/toxres\/tfad064","type":"journal-article","created":{"date-parts":[[2023,8,8]],"date-time":"2023-08-08T06:20:06Z","timestamp":1691475606000},"page":"756-764","source":"Crossref","is-referenced-by-count":4,"title":["Scorpion venom heat-resistant peptide alleviates mitochondrial dynamics imbalance induced by PM2.5 exposure by downregulating the PGC-1\u03b1\/SIRT3 signaling pathway"],"prefix":"10.1093","volume":"12","author":[{"given":"Lanyi","family":"Huang","sequence":"first","affiliation":[{"name":"Dalian Medical University College of Medical Laboratory, , Dalian 116044 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingbin","family":"Xu","sequence":"additional","affiliation":[{"name":"Dalian Medical University College of Medical Laboratory, , Dalian 116044 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaiqian","family":"Duan","sequence":"additional","affiliation":[{"name":"Dalian Medical University College of Medical Laboratory, , Dalian 116044 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tuya","family":"Bao","sequence":"additional","affiliation":[{"name":"Dalian Medical University College of Medical Laboratory, , Dalian 116044 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Cheng","sequence":"additional","affiliation":[{"name":"Dalian Medical University College of Medical Laboratory, , Dalian 116044 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haimin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Dalian Medical University College of Medical Laboratory, , Dalian 116044 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Dalian Medical University College of Medical Laboratory, , Dalian 116044 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingwei","family":"Lin","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital of Dalian Medical University Department of Laboratory Medicine, , Dalian 116044 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9368-7339","authenticated-orcid":false,"given":"Fasheng","family":"Li","sequence":"additional","affiliation":[{"name":"Dalian Medical University College of Medical Laboratory, , Dalian 116044 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,8,7]]},"reference":[{"issue":"11","key":"2023103102465359800_ref1","doi-asserted-by":"crossref","first-page":"3967","DOI":"10.1007\/s10653-021-01136-1","article-title":"Long-term nonlinear relationship between PM(2.5) and ten leading causes of death","volume":"44","author":"Lin","year":"2022","journal-title":"Environ Geochem Health"},{"issue":"46","key":"2023103102465359800_ref2","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2109310118","article-title":"Air pollution interacts with genetic risk to influence cortical networks implicated in depression","volume":"118","author":"Li","year":"2021","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2023103102465359800_ref3","doi-asserted-by":"crossref","first-page":"106150","DOI":"10.1016\/j.envint.2020.106150","article-title":"Inflammation response, oxidative stress and DNA damage caused by urban air pollution exposure increase in the lack of DNA repair XPC protein","volume":"145","author":"Oliveira Alves","year":"2020","journal-title":"Environ Int"},{"issue":"1","key":"2023103102465359800_ref4","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1186\/s12974-016-0480-3","article-title":"Urban traffic-derived nanoparticulate matter reduces neurite outgrowth via TNF\u03b1 in vitro","volume":"13","author":"Cheng","year":"2016","journal-title":"J Neuroinflammation"},{"issue":"s1","key":"2023103102465359800_ref5","doi-asserted-by":"crossref","first-page":"S145","DOI":"10.3233\/JAD-180631","article-title":"Air pollution and dementia: a systematic review","volume":"70","author":"Peters","year":"2019","journal-title":"J Alzheimer's Dis: JAD"},{"key":"2023103102465359800_ref6","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.neubiorev.2020.12.022","article-title":"Preventing dementia? 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