{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:18:48Z","timestamp":1753877928495,"version":"3.41.2"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Zanthoxylum armatum DC. (ZADC) is a novel food raw material resource, offering both edible and medicinal properties. Recent research has unveiled the toxic nature of ZADC, particularly its close association with the nervous system. In a prior study, we observed that administering methanol extract of Zanthoxylum armatum DC. (MZADC) to rats via gavage at a dose of 1.038 g\/kg resulted in various neurotoxicity symptoms, including excessive salivation, reduced mobility, unsteady gait, muscle twitching, and altered respiratory rates.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Materials and methods<\/jats:title>\n                  <jats:p>We conducted cell-based research to assess the safety of ZADC and elucidate its potential toxic mechanism. In addition, we used experimental methods such as Cell Counting Kit-8, Western blot, and Flow cytometry to detect cytotoxicity in SH-SY5Y cells after intervention with MZADC.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Following exposure of SY-SY5Y cells with MZADC, a substantial decline in cell viability was observed, accompanied by a concentration-dependent increase in intracellular reactive oxygen species (ROS) levels. Additionally, MZADC induced cellular oxidative stress, leading to elevated malonic dialdehyde (MDA) and superoxide dismutase (SOD) concentrations while decreasing glutathione (GSH) levels. Furthermore, MZADC induced apoptosis at varying doses (20, 40, and 60 \u03bcg\/mL), and this effect was associated with increased Caspase-3, Bax expressions, and reduced Bcl2 and Bcl2\/Bax expressions. In addition, the investigation revealed that MZADC induced autophagy inhibition in SH-SY5Y cells by activating the mTOR signaling pathway, resulting in a decrease in LC3II\/LCI and Beclin-1, while increasing p-mTOR\/mTOR, p62.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>Consequently, this study suggests that MZADC triggers the mTOR pathway through oxidative stress in SH-SY5Y cells, ultimately leading to apoptosis. Understanding the toxicity mechanisms associated with ZADC can offer a valuable theoretical and experimental basis for its development and utilization.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/toxres\/tfae013","type":"journal-article","created":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T11:16:49Z","timestamp":1707304609000},"source":"Crossref","is-referenced-by-count":0,"title":["Study on SH-SY5Y autophagy inhibition and apoptosis induced by methanol extract of <i>Zanthoxylum armatum<\/i> DC. based on mTOR signal pathway"],"prefix":"10.1093","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8949-4099","authenticated-orcid":false,"given":"Jiafu","family":"Guo","sequence":"first","affiliation":[{"name":"School of Public Health, Chengdu University of Traditional Chinese Medicine , No. 1166, Liutai Avenue, Chengdu, Sichuan 611137 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiayu","family":"Wen","sequence":"additional","affiliation":[{"name":"School of Public Health, Chengdu University of Traditional Chinese Medicine , No. 1166, Liutai Avenue, Chengdu, Sichuan 611137 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiwen","family":"Xiang","sequence":"additional","affiliation":[{"name":"School of Public Health, Chengdu University of Traditional Chinese Medicine , No. 1166, Liutai Avenue, Chengdu, Sichuan 611137 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Huang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Southwestern Chinese Medicine Resources, Chengdu University of Traditional Chinese Medicine , No. 1166, Liutai Avenue, Chengdu, Sichuan 611137 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tingting","family":"Hu","sequence":"additional","affiliation":[{"name":"School of Public Health, Chengdu University of Traditional Chinese Medicine , No. 1166, Liutai Avenue, Chengdu, Sichuan 611137 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chaolong","family":"Rao","sequence":"additional","affiliation":[{"name":"School of Public Health, Chengdu University of Traditional Chinese Medicine , No. 1166, Liutai Avenue, Chengdu, Sichuan 611137 , China"},{"name":"Key Laboratory of Southwestern Chinese Medicine Resources, Chengdu University of Traditional Chinese Medicine , No. 1166, Liutai Avenue, Chengdu, Sichuan 611137 , China"},{"name":"R&D Center for Efficiency , Safety and Application in Chinese Materia Medica with Medical and Edible Values, , No. 1166, Liutai Avenue, Chengdu, Sichuan 611137 , China"},{"name":"Chengdu University of Traditional Chinese Medicine , Safety and Application in Chinese Materia Medica with Medical and Edible Values, , No. 1166, Liutai Avenue, Chengdu, Sichuan 611137 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2024,2,6]]},"reference":[{"issue":"4","key":"2024020711153096000_ref1","first-page":"987","article-title":"Textual research on origin of Zanthoxylum armatum and consideration of its definition","volume":"49","author":"Zhu","year":"2018","journal-title":"Chin. 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