{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T03:28:17Z","timestamp":1790479697200,"version":"4.1.0"},"reference-count":42,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2020,1,16]],"date-time":"2020-01-16T00:00:00Z","timestamp":1579132800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100000066","name":"National Institute of Environmental Health Sciences","doi-asserted-by":"publisher","award":["ES017431"],"award-info":[{"award-number":["ES017431"]}],"id":[{"id":"10.13039\/100000066","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000066","name":"National Institute of Environmental Health Sciences","doi-asserted-by":"publisher","award":["ES027015"],"award-info":[{"award-number":["ES027015"]}],"id":[{"id":"10.13039\/100000066","id-type":"DOI","asserted-by":"publisher"}]},{"name":"University of Utah Undergraduate Opportunities"},{"name":"National Institute of General Medical Sciences Diversity Supplement","award":["GM121648"],"award-info":[{"award-number":["GM121648"]}]},{"name":"University of Utah ARUP"},{"DOI":"10.13039\/100000097","name":"National Center for Research Resources","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000097","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000097","name":"NCRR","doi-asserted-by":"publisher","award":["1S10RR024761-01"],"award-info":[{"award-number":["1S10RR024761-01"]}],"id":[{"id":"10.13039\/100000097","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Mucus hypersecretion is a pathological feature of acute inflammatory and chronic obstructive pulmonary diseases. Exposure to air pollutants can be a cause of pathological mucus overproduction, but mechanisms by which different forms of air pollutants elicit this response are not fully understood. In this study, particulate matter (PM) generated from burning pine wood and other types of biomass was used to determine mechanisms by which these forms of PM stimulate mucin gene expression and secretion by primary human bronchial epithelial cells (HBECs). Biomass PM &amp;lt; 2.5\u2009\u03bcm generated from pine wood and several other fuels stimulated the expression and secretion of the gel-forming glycoprotein MUC5AC by HBECs. Muc5ac gene induction was also observed in mouse airways following subacute oropharyngeal delivery of pine wood smoke PM. In HBECs, MUC5AC was also induced by the transient receptor potential ankyrin-1 (TRPA1) agonists\u2019 coniferaldehyde, a component of pine smoke PM, and allyl isothiocyanate, and was attenuated by a TRPA1 antagonist. Additionally, inhibition of epidermal growth factor receptor (EGFR\/ErbB1) and the EGFR signaling partners p38 MAPK and GSK3\u03b2 also prevented MUC5AC overexpression. Collectively, our results suggest that activation of TRPA1 and EGFR, paired with alterations to p38 MAPK and GSK3\u03b2 activity, plays a major role in MUC5AC overproduction by bronchial epithelial cells exposed to biomass smoke PM. These results reveal specific processes for how biomass smoke PM may impact the human respiratory system and highlight potential avenues for therapeutic manipulation of lung diseases that are affected by air pollutants.<\/jats:p>","DOI":"10.1093\/toxsci\/kfaa006","type":"journal-article","created":{"date-parts":[[2020,1,10]],"date-time":"2020-01-10T20:10:32Z","timestamp":1578687032000},"page":"278-290","source":"Crossref","is-referenced-by-count":41,"title":["Wood Smoke Particles Stimulate MUC5AC Overproduction by Human Bronchial Epithelial Cells Through TRPA1 and EGFR Signaling"],"prefix":"10.1093","volume":"174","author":[{"given":"Tosifa A","family":"Memon","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Toxicology, Center for Human Toxicology, University of Utah, Salt Lake City, Utah"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nam D","family":"Nguyen","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Toxicology, Center for Human Toxicology, University of Utah, Salt Lake City, Utah"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine L","family":"Burrell","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Toxicology, Center for Human Toxicology, University of Utah, Salt Lake City, Utah"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abigail F","family":"Scott","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Toxicology, Center for Human Toxicology, University of Utah, Salt Lake City, Utah"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marysol","family":"Almestica-Roberts","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Toxicology, Center for Human Toxicology, University of Utah, Salt Lake City, Utah"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuel","family":"Rapp","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Toxicology, Center for Human Toxicology, University of Utah, Salt Lake City, Utah"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cassandra E","family":"Deering-Rice","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Toxicology, Center for Human Toxicology, University of Utah, Salt Lake City, Utah"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher A","family":"Reilly","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Toxicology, Center for Human Toxicology, University of Utah, Salt Lake City, Utah"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,1,16]]},"reference":[{"key":"2020032609574864000_kfaa006-B1","doi-asserted-by":"crossref","first-page":"2485","DOI":"10.1523\/JNEUROSCI.5369-07.2008","article-title":"Transient receptor potential A1 is a sensory receptor for multiple products of oxidative stress","volume":"28","author":"Andersson","year":"2008","journal-title":"J. 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